Corresponding Author: Abdulaziz Rabiu Abdulkadir, Department of Agriculture and Biotechnology, Faculty of Bioresources and Food Industry, Universiti Sultan Zainul Abidin, (UNISZA) Tembila campus Besut, Terengganu, Malaysia In Vitro Anti-Oxidant Potential, Total Phenolic Content and Total Flavonoi... (original) (raw)
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Background: Plant source antioxidant plays an important role in regulating free radicals from the body. So it prevents damage to cell which can be caused by free radicals. Objective: This study was carried out to determine in vitro antioxidant potential, total phenolic content and total flavonoid content in flower and seed of Moringa oleifera. Results: Antioxidant activity of the extract was analysed by DPPH radical scavenging assay. Methanolic flower extract shows high potential free radical scavenging activity with IC50 value of 425 μg/ml. compare to methanolic seed extract that showed no IC50 value. The highest phenolic and flavonoid contents were observed from methanolic flower extract which was 48.04±2.44mg/g GAE and 14.27±0.62mg/g QAE respectively. Conclusion: The result proves that Moringa oleifera is an excellent source of antioxidants that can be used to reduce the effect of oxidative damages.
IN VITRO ANTIOXIDANT ACTIVITY OF MORINGA OLEIFEM LEAVES
Five different extracts (Methanol, Ethanol, Petroleum ether, n-hexane and Chloroform) of Moringa oleifera leaves were scrutinized to see the antioxidant and free radical scavenging activity. In this study free radical scavenging potential of five €xtracts ofthe leaves of M. oleifera was assessed by observing its capabiliq/ for scavenging 2,2-diphenyl-lpicrylhydrazyl (DPPH) radical, hydrogen peroxide radical, nitric oxide (NO) radical, as well as its ability in reducing power capacity assessment, Cupric Reducing Antioxidant Capacity, using appropriate assay systems compared to natural and synthetic antioxidants. Total antioxidant capacity and phenolic, flavonoid contents were determined spectrophotometrically. The highest lC5a value was showed by chloroform extract with a value of 47.481 pglml followed by ethanol and methanol having value of62.09 and 68.321 respectively as opposed to that of the scavenging effects of ascorbic acid and BHT of 5.698 and 8.816 respectively in DPPH free radical scavenging activity. Chloroform extract showed highest activity having lCjo value of 48.843 and 94.506 pg/ml in hydrogen peroxide and nitric oxide scavenging assay respectively. The fractions showed good reducing power and cupric reducing capacity with increasing concentration again taking chloroform extract in the top position. The Chloroform extract yielded (147.261+3.061) mg/gm gallic acid equivalent phenolic content and (137.63513.258) ng/gm Quercetin equivalent flavonoid content that was highest among five extracts. Chlorofom extract of Moringq oleifera was found lo possess the highest tolal antioxidant capacity (58.175+3.122) followed by ethanol (53.925x5.25) and methanol (50.675+3.699) mg/gm Ascorbic Acid Equivalent respectively. A linear corelation appeared betw€en the total antioxidant capacity and the total phenolic contents ofthe extracts with good conelation coefficient (R2 = 0.891). n-hexane and petroleum ether extract showed least activity in all the measures. The results obtained beacon that Moringa oleifera is a potential source of antioxidants and thus could prevent many radical related diseas€s.
In vitro Antioxidant Activities of Different parts of the Plant Moringa oleifera Lam
The aim of the present study was to evaluate and compare the in vitro antioxidant activity of different parts of the plant Moringa oleifera lam. Antioxidant activity was measured based on the DPPH radical scavenging assay, Nitric oxide scavenging assay, determination of total phenol, total flavonoids, total antioxidant content and reducing power assay. Ethyl acetate fraction of both bark and leaves showed potent free radical scavenging activity with IC 50 value of 14.47 and 29.91 (µg/ml) respectively. The IC 50 value of standard (Ascorbic acid) was 33.77 (µg/ml). It was observed that ethyl acetate fraction of fruit contain highest amount of phenolics (613.023±4.11108) expressed as mg Gallic acid equivalents (GAE)/gm of plant extract. In case of total flavonoid content, pet ether fraction of bark contains 2453.1±16.4443 (expressed as mg of quercetin equivalents/gm of plant extract). All fractions contains significant amount of ascorbic acid equivalents ranging from 445.581±0.8222-1162.44±82.222 as mg of ascorbic acid equivalents per gram of plant extract. In case of nitric oxide scavenging assay, ethyl acetate and chloroform fractions of bark and fruit of Moringa oleifera showed potential antioxidant effect having IC 50 values ranging from 2.9-87.83 µg/ml. For reducing power assay, all extracts showed increase in the absorbance with increase in concentration. The present study supplements and at the same time compared the presence of antioxidant compounds in different parts of the plant M. oleifera. The next step is to identify the individual compound and their role in different chronic diseases.
Antioxidant activity of Moringa oleifera seed extracts
Oriental Pharmacy and Experimental Medicine, 2018
The present research evaluates the phytochemical and antioxidant activity of Moringa oleifera Lam. seed kernel grown in Bangladesh. M. oleifera seed kernel was extracted with methanol, acetone and water individually. The phytochemical content was evaluated through the determination of total phenolic, total flavonoid and total tannin contents. In vitro antioxidant capacity was determined following four complementary methods: DPPH (2,2-diphenyl-1-picrylhydrazyl, ABTS{2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)}, and NO (nitric oxide) free radical scavenging and reducing power tests. The total phenolic contents 60.99 ± 0.153, 30.78 ± 0.101 and 90.97 ± 0.134 mg gallic acid eq./g dry extract, total flavonoids contents 10.13 ± 0.171, 13.32 ± 0.101 and 221.76 ± 0.221 mg quercetin eq./g and total tannins contents 97.10 ± 0.153, 73.91 ± 0.107 and 21.74 ± 0.086 mg gallic acid eq./g dry extract were found to be present in methanol, acetone and water extracts respectively. Among the three extracts, the water extract exhibited significant activities for scavenging DPPH, ABTS and NO free radicals with EC 50 values 36.89 ± 0.154, 13.20 ± 0.049 and 217.95 ± 0.327 μg/mL respectively against the standard antioxidant compound ascorbic acid and butylated hydroxy anisole. The results of this research revealed that among the three extracts of M. oleifera seed kernel the water extract exhibited significant free radical scavenging activity. Reducing power, total phenolic and total flavonoid contents were also observed to be significant for the water extract. So in contrast of all the results of this study it can be concluded that among the three extracts (methanol, acetone and water) the water extract possessed potent antioxidant activity which support the use of M. oleifera seed kernel as natural antioxidant.
Antioxidant Activity of Moringa oleifera Tissue Extracts
Phytotherapy Research, 2012
Moringa oleifera is an important source of antioxidants, tools in nutritional biochemistry that could be beneficial for human health; the leaves and flowers are used by the population with great nutritional importance. This work investigates the antioxidant activity of M. oleifera ethanolic (E1) and saline (E2) extracts from flowers (a), inflorescence rachis (b), seeds (c), leaf tissue (d), leaf rachis (e) and fundamental tissues of stem (f). The radical scavenging capacity (RSC) of extracts was determined using dot-blots on thin layer chromatography stained with a 0.4 mM 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) solution; spectrophotometric assays were recorded (515 nm). Antioxidant components were detected in all E1 and E2 from a, b and d. The best RSC was obtained with E1d; the antioxidants present in E2 reacted very slowly with DPPH. The chromatogram revealed by diphenylborinate-2-ethylamine methanolic solution showed that the ethanolic extract from the flowers, inflorescence rachis, fundamental tissue of stem and leaf tissue contained at least three flavonoids; the saline extract from the flowers and leaf tissue revealed at least two flavonoids. In conclusion, M. oleifera ethanolic and saline extracts contain antioxidants that support the use of the plant tissues as food sources.
The purpose of this study was to judge the antioxidant activity and brine shrimp lethality bioassay followed by phytochemical screening of five different extracts of Moringa oleifera leaves. Preliminary Phytochemical screening of the crude extracts of Moringa oleifera leaf revealed the presence of different kind of chemical groups such as Flavonoids, tannin, Saponin, alkaloids, glycosides, carbohydrate and Triterpenoids. The leaf exhibited significant DPPH free radical scavenging activity with highest IC 50 value showed by chloroform extract with a value of 47.481 µg/ml followed by ethanol and methanol having value of 62.09 and 68.321 respectively as opposed to that of the scavenging effects of ascorbic acid and BHT of 5.698 and 8.816 respectively. Dried leaf of Moringa oleifera were subjected to brine shrimp lethality bioassay and the LC 50 values of methanol, ethanol, petroleum ether, n-hexane and chloroform were found to be 0.747µg/ml, 0.712 µg/ml, 1.632 µg/ml, 2.163 µg/ml and 0.633 µg/ml respectively. The data obtained in the present study suggests that the extracts of Moringa oleifera leaves have potent antioxidant activity against free radicals and significant cytotoxic activity that can be used in disease prevention.
BioMed Research International
Medicinal plants are used to control and remediate oxidative stress related diseases caused by free radicals. Thus, these plants find their use as remedy. Moringa oleifera is an extremely valued plant for its medicinal properties. Herein, two indigenously produced accessions of Moringa oleifera seeds [originated from Multan (M-Mln) and India (PKM1)] were investigated for their antioxidant properties by 2.2-Diphenyl-1picrylhydrazyl (DPPH) assay, total phenolics content and total flavonoids content. The presence of various phenolics as well as flavonoids was further confirmed by high performance liquid chromatography. Moreover, fourier transform infrared spectroscopy detected the presence of various functional groups. In conclusion, these findings revealed that the methanol extract of M-Mln variety seeds showed high antioxidant potential, having IC50 value of 84 μg/ml. While, hexane extract of PKM1 showed least activity. The methanol extract of M-Mln was found to show highest total ph...
Comparative analysis of phenolic contents and total antioxidant capacity of Moringa oleifera Lam
Pharmacognosy Journal, 2014
Introduction: Accumulation of reactive species higher than permissible limits in biological systems may lead to various degenerative disorders due to oxidative damage. Materials And Methods: Oxidation is a serious concern faced by the food industry causing deterioration of shelved-food quality. Antioxidant compounds like polyphenolics scavenge such free radicals and thus protect against oxidative stress. Consumption of polyphenol-rich plants as dietary component confers protection against such cellular damage. Results: Present study explores antioxidant capacity, total phenolic content (TPC) and total flavonoid content (TFC)of different extracts prepared from various parts of Moringa oleifera Lam. Higher TPC, TFC and antioxidant activity was shown by methanolic extracts followed by aqueous, petroleum benzene and chloroform extracts.The present study suggests that all the extracts might act as radical scavengers to certain extent possibly due to presence of polyphenolic compounds. Conclusion: M. oleifera exhibits strong antioxidant activity and could serve as prospective source of natural antioxidants to food and health industries.
Antioxidant Activity of Moringa oleifera Extracts
Indonesian Journal of Chemistry, 2016
Moringa oleifera have been evaluated for its antioxidant activity. M. oleifera leaves were extracted with methanol, ethyl acetate, dichloromethane and n-hexane. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay and an improved 2,2’-azino-bis-[3-ethylbenzothiazoline sulphonate] (ABTS) radical cation decolorization assay in vitro. Trolox was used as standard with IC50 5.89 μg/mL in DPPH assay and 3.06 μg/mL in ABTS assay. The methanol extract showed the highest free radical scavenging activity with IC50 value of 49.30 μg/mL in DPPH assay and 11.73 μg/mL in ABTS assay. This study provided that M. oleifera leaves possess antioxidant.
TOTAL POLYPHENOLIC CONTENT AND ANTIOXIDANT PROPERTIES OF MORINGA OLEIFERA LEAF EXTRACTS
The study was carried out to evaluate the relative antioxidant properties and polyphenol contents of partially purified fractions of Moringa oleifera leaves extracts. The total phenolic, total flavonoid, anthocyanin, proanthocyanidine and tannin contents of the crude methanolic extract, aqueous fraction and ethyl acetate fraction were determined using established methods, while the antioxidant properties of the test fractions were evaluated using five in vitro radical scavenging assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, nitric oxide inhibitory assay, lipid peroxidation assay, reductive potential assay, and the ferric reducing ability of plasma (FRAP) assay. The highest radical scavenging effect and polyphenol contents were observed in the ethyl acetate fractions than the other fractions: the order of activity for all the assays was ethyl acetate reaction > crude extract > aqueous extract. The results obtained in the present study indicates that M. oleifera could be a potential source of natural antioxidant and could be applied as a functional food as regard its relatively low tannin content.