Lindnera wuzhiensis sp. nov., a novel ascomycetous yeast species (original) (raw)
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Kazachstania taianensis sp. nov., a novel ascomycetous yeast species from orchard soil
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2010
Three teleomorphic ascomycetous yeast isolates (TA11TR-1T, TA11TR-4 and TA11TR-6) from orchard soil from Tai'an, Shandong province, China, were shown to represent a novel species within the genus Kazachstania based on phenotypic characterization and sequence analyses of the 18S rRNA gene, internal transcribed spacer (ITS) regions and 26S rDNA gene D1/D2 domain. The name Kazachstania taianensis sp. nov. (type strain TA11TR-1T =AS 2.4160T =CBS 11405T) is proposed. K. taianensis sp. nov. clustered in a branch together with Kazachstania sinensis, Kazachstania naganishii and the Kazachstania telluris complex with moderate bootstrap support in the neighbour-joining tree reconstructed from combined 18S and D1/D2 sequences. The novel species possessed unusual ITS 1 (338 bp) and ITS 2 (488 bp) sequences. The total length of the ITS–5.8S rDNA gene region of the species was 983 bp, being much longer than those of other ascomycetous yeast species described so far.
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2008
In a taxonomic study on the ascomycetous yeasts isolated from plant materials collected in tropical forests in Yunnan and Hainan Provinces, southern China, four strains isolated from tree sap (YJ2ET) and flowers (YF9ET, YWZH3CT and YYF2AT) were revealed to represent four undescribed yeast species. Molecular phylogenetic analysis based on the large subunit (26S) rRNA gene D1/D2 domain sequences showed that strain YJ2ET was located in a clade together with Candida haemulonii and C. pseudohaemulonii. Strain YF9ET was most closely related to C. azyma and strain YWZH3CT to C. sorbophila and C. spandovensis. Strain YYF2AT was clustered in a clade containing small-spored Metschnikowia species and related anamorphic Candida species. The new strains differed from their closely related described species by more than 10% mismatches in the D1/D2 domain. No sexual states were observed for the four strains on various sporulation media. The new species are therefore assigned to the genus Candida and described as Candida alocasiicola sp. nov. (type strain, YF9ET = AS 2.3484T = CBS 10702T), Candida hainanensis sp. nov. (type strain, YYF2AT = AS 2.3478T = CBS 10696T), Candida heveicola sp. nov. (type strain, YJ2ET = AS 2.3483T = CBS 10701T) and Candida musiphila sp. nov. (type strain, YWZH3CT = AS 2.3479T = CBS 10697T).
Locally isolated yeasts from Malaysia: identification, phylogenetic study and characterization
Acta Biochimica Polonica
Yeasts are a convenient platform for many applications. They have been widely used as the expression hosts. There is a need to have a new yeast expression system to contribute the molecular cloning demands. Eight yeast isolates were screened from various environment sources and identified through ribosomal DNA (rDNA) Internal Transcribed Spacer (ITS). Full sequence of the rDNA ITS region for each isolate was BLASTed and phylogenetic study was constructed by using MEGA4. Among the isolates, isolate WB from 'ragi' (used to ferment carbohydrates) could be identified as a new species in order Saccharomycetales according to rDNA ITS region, morphology and biochemical tests. Isolate SO (from spoiled orange), RT (rotten tomato) and RG (different type of 'ragi') were identified as Pichia sp. Isolates R1 and R2, S4 and S5 (from the surrounding of a guava tree) were identified as Issatchenkia sp. and Hanseniaspora sp., respectively. Geneticin, 50 µg/mL, was determined to be th...