The value of the D1/D2 and internal transcribed spacers (ITS) domains for the identification of yeast species belonging to the genus Yamadazyma (original) (raw)
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International journal of systematic and evolutionary microbiology, 2015
Two yeast strains representing two novel yeast species were isolated from frass of an unidentified insect (ST-78) and the external surfaces of rice leaves (YE170) collected in Thailand. The two strains are genetically, morphologically and phenotypically distinct from recognized species and were found to represent two novel Yamadazyma species although formation of ascospores was not observed. In terms of pairwise sequence similarity of the D1/D2 region of the large subunit (LSU) rRNA gene, the closest species of the strain ST-78 was Candida lessepsii but with 3.8% nucleotide substitutions, while the closest species of the strain YE170 was the strain ST-78 but with 4.3% nucleotide substitutions. Analysis of the sequences of the internal transcribed spacer 1 and 2 (ITS1-2) regions revealed that strain ST-78 differed from C. lessepsii by 8.8% nucleotide substitutions and from strain YE170 by 9.4% nucleotide substitutions. The result of pairwise sequence similarity of the D1/D2 and ITS1-...
Yamadazyma riverae sp. nov., a yeast species isolated from plant materials
International Journal of Systematic and Evolutionary Microbiology, 2015
Nine strains of a novel yeast species were isolated from rotting wood, tree bark, ant nests or living as endophytes in leaves of Vellozia gigantea. Analysis of the sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene showed that this species was related to Candida insectorum in the Yamadazyma clade. The novel species differed from closely related species by 10 and 11 substitutions in the ITS region and the D1/D2 domains of the large subunit of the rRNA gene, respectively. The species is heterothallic and forms asci with one to two hat-shaped ascospores. The name Yamadazyma riverae sp. nov. is proposed for the novel species. The type strain is UFMG-CM-Y444T ( = CBS 14121T) and the allotype strain is TT12 ( = CBS 14098 = UFMG-CM-Y577). The Mycobank number is MB 813221.