Genome Sequence of Bacillus halotolerans Strain MS50-18A with Antifungal Activity against Phytopathogens, Isolated from Saline Soil in San Luís Potosí, Mexico (original) (raw)
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Microbial pathogenesis, 2018
Bacillus halotolerans is a rhizobacterium with the potential to promote plant growth and tolerance to drought and salinity stress. Here, we present the complete genome sequence of B. halotolerans ZB201702, which consists of 4,150,000 bp in a linear chromosome, including 3074 protein-coding sequences, 30 rRNAs, and 85 tRNAs. Genome analysis revealed many putative gene clusters involved in defense mechanisms. Activity analysis of the strain under salt and simulated drought stress suggests tolerance to abiotic stresses. The complete genome information of B. halotolerans ZB201702 could provide valuable insights into rhizobacteria-mediated plant salt and drought tolerance and rhizobacteria-based solutions for abiotic stress agriculture.
Genomic Mining and Physiological Characterization of new Halophilic Plant Growth Promoting Bacilli
2021
Claudia Petrillo1, † , Stefany Castaldi1, † , Mariamichela Lanzilli2, Matteo Selci1, Angelina Cordone1, Donato Giovannelli1,3,4,5,6,7, Rachele Isticato1,* 1Department of Biology, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Naples, Italy 2Institute of Biomolecular Chemistry (ICB), CNR, Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy 3Department of Life Sciences, DISVA, Polytechnic University of Marche, Ancona, Italy 4National Research Council – Institute of Marine Biological Resources and Biotechnologies CNRIRBIM, Ancona, Italy 5Department of Marine and Coastal Science, Rutgers University, New Brunswick, NJ, USA 6Marine Chemistry & Geochemistry Department Woods Hole Oceanographic Institution, MA, USA 7Earth-Life Science Institute, Tokyo Institute of Technology, Tokyo, Japan
2020
Halophilic microorganisms thrive in conditions of high salt concentration and osmotic stress. Due to these reasons, halophilic microorganisms with ability to fix nitrogen or mobilize phosphate represent a potential as bio-fertilizer to increase crop growth and yield in saline soils. In this study, 35 different halophilic bacteria were isolated from saline soils of Coahuila State, Mexico. These bacterial isolates were characterized, and evaluated for their halophilic potential by growth kinetics, hydrolysis halos formation and in vitro sodium capture. Based on the results, seven potential strains for saline conditions were selected and were identified by 16S rDNA sequencing analysis Phylogenetic relationship of the selected strains as well as with other halophilic microorganisms was determined. Six of the identified strains were Halobacillus trueperi, Bacillus licheniformis, Bacillus pumilus, Staphylococcus succinus, Bacillus atrophaeus and Bacillus subtilis. Other two strains were i...
CHARACTERIZATION AND SELECTION OF HALOPHILIC MICROORGANISMS ISOLATED FROM MEXICAN SOILS
Halophilic microorganisms thrive in conditions of high salt concentration and osmotic stress. Due to these reasons, halophilic microorganisms with ability to fix nitrogen or mobilize phosphate represent a potential as bio-fertilizer to increase crop growth and yield in saline soils. In this study, 35 different halophilic bacteria were isolated from saline soils of Coahuila State, Mexico. These bacterial isolates were characterized, and evaluated for their halophilic potential by growth kinetics, hydrolysis halos formation and in vitro sodium capture. Based on the results, seven potential strains for saline conditions were selected and were identified by 16S rDNA sequencing analysis Phylogenetic relationship of the selected strains as well as with other halophilic microorganisms was determined. Six of the identified strains were Halobacillus trueperi, Bacillus licheniformis, Bacillus pumilus, Staphylococcus succinus, Bacillus atrophaeus and Bacillus subtilis. Other two strains were identified Halobacillus sp. and Oceanobacillus sp., moderately halophilic organisms.
bioRxiv, 2021
Massive application of chemical fertilizers and pesticides has been the main strategy used to cope with the rising crop demands in the last decades. The indiscriminate use of chemicals while providing a temporary solution to food demand has led to a decrease in crop productivity and an increase in the environmental impact of modern agriculture. A sustainable alternative to the use of chemicals for crop production is the use of microorganisms naturally capable of enhancing plant growth and protecting crops from pests, known as Plant-Growth-Promoting Bacteria (PGPB). Aim of the present study was to isolate and characterize PGPB from salt-pans sand samples able to ameliorate plant fitness. To survive high salinity, salt-tolerant microbes produce a broad range of compounds with heterogeneous biological activities that are potentially beneficial for plant growth. We have isolated and screened in vitro a total of 20 halophilic spore-forming bacteria for phyto-beneficial traits and compare...
Journal of biotechnology, 2015
Bacillus velezensis G341 can suppress plant pathogens by producing antagonistic active compounds including bacillomycin D, fengycin, and (oxy) difficidin. The complete genome sequence of this bacterium was characterized by one circular chromosome of 4,009,746bp with 3,953 open reading frames. The genome contained 36 pseudogenes, 30 rRNA operons, and 95 tRNAs. This complete genome sequence provides an additional resource for the development of antimicrobial compounds.
Microorganisms
The endophytic strain Cal.l.30, isolated from the medicinal plant Calendula officinalis, was selected among seven Bacillus strains with plant growth promoting activity and strong biological potential against the postharvest fungal pathogen Botrytis cinerea. Treatment by inoculating Cal.l.30 bacterial cell culture or cell free supernatant on harvested grapes and cherry tomato fruits, significantly reduced gray mold disease severity index and disease incidence. Based on 16S rRNA sequence analysis and whole genome phylogeny, Cal.l.30 was identified as Bacillus halotolerans. Genome mining revealed that B. halotolerans Cal.l.30 is endowed with a diverse arsenal of secondary metabolite biosynthetic gene clusters (SM-BGCs) responsible for metabolite production with antimicrobial properties. A sub-set of the identified SM-BGCs (mojavensin A, ‘bacillunoic acid’) appears to be the result of recent horizontal gene transfer events. Its genome was also mined for CAZymes associated with antifunga...