Microbiome-Gut-Brain-Axis communication influences metabolic switch in the mosquito Anopheles culicifacies (original) (raw)

Abstract

Background: Periodic ingestion of a protein-rich blood meal by adult female mosquitoes causes a drastic metabolic change in their innate physiological status, which is referred to as ‘metabolic switch. Although the down-regulation of olfactory factors is key to restrain host-attraction, how the gut ‘metabolic switch’ modulates brain functions, and resilience physiological homeostasis remains unexplored. Methods: To uncover a possible correlation of gut metabolic switching and brain function, we carried out a comparative RNAseq analysis of naïve and blood-fed mosquito’s brain. Spatio-temporal expression of neuro-signaling and neuro-modulatory genes was monitored through Real-Time PCR. To establish a proof-of-concept, we followed LC/MS-based absolute quantification of different neurotransmitters (NT) and compared their levels in the brain as well as in the gut of the mosquitoes. To correlate how microbiome influences gut-brain-axis communication, we performed a comparative gut metagen...

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