Antioxidant Potential and its Correlation with the Contents of Phenolic Compounds and Flavonoids of Methanolic Extracts from Different Medicinal Plants (original) (raw)
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2010
Os benefícios de saúde atribuídos ao chá verde e suas catequinas têm levado a um grande aumento de produtos comerciais à base de chá verde no mercado. Os objetivos deste trabalho foram analisar e comparar estes produtos em relação ao conteúdo de fenólicos totais e capacidade antioxidante in vitro, incluindo produtos na forma de sachês, folhas desidratadas e bebidas prontas. O conteúdo de fenólicos totais em 200 mL (1 xícara) dos diferentes chás variou de 90 a 341 mg equivalentes de catequina, e o maior e o menor valor foram apresentados pelas bebidas prontas. As infusões preparadas a partir dos sachês tiveram conteúdos variando de 96 a 201 mg.200 mL-1 , e não foram encontradas diferenças significativas entre os lotes. Nos ensaios de sequestro do radical DPPH e ORAC (Oxygen Radical Absorbing Capacity), houve ampla variação dos resultados entre as diferentes preparações de chás, de 23 a 131 mmoles equivalentes de Trolox (ET).200 mL-1 (DPPH), e de 1,2 a 5,1 mmoles ET.200 mL-1 (ORAC), e novamente não houve diferenças entre as infusões e/ou bebidas comerciais prontas. Entretanto, a capacidade antioxidante das bebidas prontas para consumo é parcialmente devido à presença de outros compostos não fenólicos como o ácido ascórbico.
Avaliação do potencial antioxidante de hortaliças orgânicas durante o armazenamento a frio
Revista de Administração da UFSM
The relation between an antioxidant-rich diet with protection against some diseases such as Alzheimer's and cardiovascular diseases has increased research on the evaluation of these components in food. Another factor related to some diseases is the consumption of food produced from conventional agriculture with the excessive use of agrochemicals and the search for food free of pesticides increases the demand for products grown in organic systems. Aiming to relate these two themes, the present study aimed to produce a kit of organic vegetables and evaluate antioxidant activity during storage at reduced temperatures. The results indicate a sudden drop in antioxidant activity and phenolic compound concentration in the first month of storage.
Gostaria de agradecer primeiramente a Deus por todas as oportunidades que me julgou capaz de receber. À minha família, que sempre me deu incentivo e apoio para que conseguisse concluir mais essa etapa da minha vida. Agradeço em especial a minha mãe, a qual obrigada é muito pouco para expressar toda minha gratidão e admiração e também ao meu irmão pela ajuda financeira durante todo esse período. À minha orientadora Profa. Maria Inés Genovese, pela oportunidade, dedicação, incentivo, paciência e exemplo de pesquisadora. Ao Prof. Thomas P. Ong pela possibilidade de utilização da sala de cultura celular, o que permitiu que este trabalho fosse desenvolvido e à sua aluna Gabriela R. Costa pela ajuda, treinamento e amizade.
Millennium: Journal of International Studies, 2019
RESUMO Introdução: Amores-perfeitos (Viola×wittrockiana) são uma fonte rica de antioxidantes naturais com efeitos benéficos para a saúde humana. Objetivos: O objetivo do presente estudo foi investigar a influência dos solventes (água, metanol e água:acetona (6:4, v/v)) na extracção de compostos bioactivos e actividade antioxidante de extractos de amores-perfeitos. Métodos: Os compostos bioactivos analisados foram os seguintes: flavonóides, taninos hidrolisáveis e antocianinas monoméricas, bem como, os fenóis totais através da avaliação da capacidade redutora total. A actividade antioxidante foi avaliada pelos métodos de sequestro do radical livre 2,2-difenil-1-picrilhidrazilo (DPPH) e poder redutor. Uma análise de componentes principais (PCA) foi realizada para diferenciar os extractos dos amores-perfeitos. Resultados: Os solventes que produziram extractos com os maiores teores de taninos hidrolisáveis e capacidade redutora total foram os de metanol e água: acetona (6:4, v/v). Para extrair os maiores teores de antocianinas monoméricas deve-se usar metanol (5.93 mg Cy 3-glu/ g de massa seca), enquanto que para extrair flavonóides, a água:acetona (6:4, v/v) é preferível (115 mg QE/g de massa seca). A água mostrou ser o solvente menos eficaz, obtendo-se extractos com a menor actividade antioxidante. Para além disso, os extractos de metanol e água:acetona foram claramente separados dos aquosos através da análise por PCA. Conclusões: Os resultados mostraram que a extracção de compostos bioactivos e a actividade antioxidante de flores de amoresperfeitos são influenciadas pelo solvente usado.
Antioxidant activities of phenolics, flavonoids and vitamin C
2013
Field experiment was conducted to study the effect of organic and bio-organic fertilizers on dry weight; yield, total phenolics (TPC), total flavonoids (TFC), vitamin C and on their antioxidant activities of two sweet fennel cultivars Dolce and Zefa fino. Results strongly showed that there were significant differences between sweet fennel cultivars. Generally the highest values of all parameters were obtained when fennel plants were supplemented with 50% NPK + 50% organic fertilizer and bio fertilizer when compared with control treatment. The highest values of TPC, TFC and Vit. C were recorded by Zefa fino cultivar when received 50% NPK + 50% organic treatment. The antioxidant activities of both cultivars were evaluated and Dolce cultivar showed the highest DPPH Å scavenging activity expressed as IC 50 compared with Zefa fino cultivar. In addition, Dolce cultivars exhibited the highest value for Fe 2+-chelating activities for organic and bio-organic fertilizers followed by Zefa fino when compared to control treatment. Dolce cultivar generally showed superiority than Zefa fino in all measured parameters.
FLAVONOIDS AND PHENOLIC ACIDS AS ANTIOXIDANTS IN PLANTS AND HUMAN HEALTH Review Article
2012
Flavonoids and phenolic acids are the most persistent groups of plant phenolics therefore they play significant role in plants and human health, thus it is important to better understanding of flavonoid awareness with biological behavior that could indicate their potentials as therapeutic agents. Plants and herbs consumed by humans may contain different phenolic acid and flavonoid components therefore the effect of dietary phenolics is currently of great interest due to their antioxidative activities although Phenolic acids and flavonoids function as reducing agents and free radical scavengers. So it will be significant to examine the possible role of flavonoids and phenolic acids in disease prevention, especially the current status of the subject matter and future prospects, in this review.
Actually there is an increase on infusion consumption due their beneficial properties, which are attributable to the presence of phenolic compounds. By this reason, the aim of this study was to assess phenolic content, antioxidant capacity and anti-inflammatory capacity of commercial infusions of peppermint (Mentha piperita L.), lemon grass (Cymbopogon citratos), chamomile (Matricaria chamomilla L.), arnica (Heterotheca inuloides) and boldo (Peumus boldus Molina) obtained in supermarket in 2009. Phenols content and antioxidant capacity were defined by spectrophotometry techniques. Also, identification and quantification of phenolic compound was made by HPLC. The anti-inflammatory activity was assessed by inhibition of ciclooxigenasa-2 (COX-2) enzyme. Boldo infusion had greatest content of phenolic compounds and greater antioxidant capacity measured by ABT techniques, followed by chamomile infusions by FRAP method. In assessed infusions ten different compounds were identified, findin...
2008
Due to differences in the functional quality of natural extracts, we have also faced differences in their effectiveness. So, it was intended to assess the antioxidant activity of natural extracts in order to attain their functional quality. It was observed that all the extracts (brown and green propolis, Ginkgo biloba and Isoflavin Beta®) and the standard used (quercetin) showed antioxidant activity in a dose-dependent manner with IC 50 values ranging from 0.21 to 155.28 μg mL-1 (inhibition of lipid peroxidation and scavenging of the DPPH • assays). We observed a high correlation (r 2 = 0.9913) among the antioxidant methods; on the other hand, the antioxidant activity was not related to the polyphenol and flavonoid content. As the DPPH • assay is a fast method, presents low costs and even has a high correlation with other antioxidant methods, it could be applied as an additional parameter in the quality control of natural extracts. RESUMEN. "Evaluación de la actividad antioxidante como parámetro adicional para lograr la calidad funcional de extractos naturales". Debido a las diferencias en la calidad funcional de extractos naturales, nosotros hemos advertido también diferencias en su efectividad, por lo que se pretende estimar la actividad antioxidante de extractos naturales para lograr su calidad funcional. Fue observado que todos los extractos (propolis marrón y verde, Ginkgo biloba e Isoflavin Beta®) y el patrón usado (quercetina) mostraron actividad antioxidante de una manera dosis-dependiente con valores de IC 50 que van de 0,21 a 155,28 al μg mL-1 (inhibición de la peroxidación lipídioca y captación de radicales libres del DPPH •). Se observó una alta correlación (r 2 = 0,9913) entre los métodos antioxidantes y por otro lado la actividad antioxidante no estuvo relacionada con el contenido del polifenoles ni de flavonoides. Como el análisis del DPPH • es un método rápido, presenta costos bajos e incluso tiene una correlación alta con otros métodos antioxidantes, este método podría utilizarse como un parámetro adicional en el control de calidad de extractos naturales.
Efecto del potasio en el contenido fenólico y capacidad antioxidante de Ocimum basilicum L
Revista Mexicana de Ciencias Agrícolas
En la última década, se han realizado numerosos esfuerzos para incrementar el contenido de compuestos fenólicos y la capacidad antioxidante de plantas que vayan directo al consumo humano, empleando para esto diversos métodos de cultivo; sin embargo, los resultados han sido discrepantes acerca de la calidad de los frutos y el rendimiento obtenido, concluyendo que el alto contenido de compuestos fenólicos y la capacidad antioxidante no ha sido garantía de alto rendimiento de los cultivos. Por lo tanto, el objetivo de este estudio fue determinar un posible aumento del contenido en compuestos fenólicos totales, capacidad antioxidante total y rendimiento de tres genotipos de albahaca (Sweet Lemon, Red Rubin y Cinnamon) producidos bajo diferentes niveles de potasio (7, 9, 11 y 13 mM). Adicionalmente, se evaluó materia seca, altura de planta y número de hojas. El trabajo se llevó a cabo en la Comarca Lagunera de Durango en el año 2014. El análisis de varianza mostró diferencia...
Journal of Plant Biochemistry & Physiology, 2018
Antioxidant property of the phenolic acids and flavonoids was assessed in multiple antioxidant assays such as 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity and deoxyribose protection assay. In all the assays the antioxidant property was found to be concentration dependent. Among phenolic acids, gentisic acid was reported to be the most effective compound with the IC 50 values of 3.56, 6.68 and 3.53 µg/ml for DPPH, ABTS and deoxyribose protection activity, respectively followed by the gallic and caffeic acids. These compounds were found to be rather more effective than the standard compounds butylated hydroxytoluene (BHT) and α-tocoferol. Among the flavonoids, catechin hydrate was reported to be the strongest antioxidant compound (IC 50 8.34, 4.93 and 5.96 µg/ml) in comparison to BHT (IC 50 17.41, 17.12 and 6.1 µg/ml) and α-tocoferol (IC 50 10.97, 32.41 and 8.37 µg/ml) for DPPH, ABTS and deoxyribose protection activity, respectively. The total antioxidant activity of these phenolic acids and flavonoids was ascertained to reflect antioxidative comparative capacity of these compounds which have been largely extracted, identified and characterized from natural sources.