Development and Validation of LC-APCI-MS Method for the Estimation of Ellagic acid in Fresh and Processed Fruit Products (original) (raw)
Related papers
Extraction, stability, and quantitation of ellagic acid in various fruits and nuts
Journal of Food Composition and Analysis, 1989
This study was undertaken to determine the amount of ellagic acid, a naturally occurring inhibitor of carcinogenesis, in various fruits and nuts. Ellagic acid was extracted from freezedried berries, pears, peaches, plums, grapes, apples, kiwi, and several nuts using either acetone/ water or methanol. The extracts were treated with trifluoroacetic acid to hydrolyze the ellagic acid glucosides, and analyzed by high-performance liquid chromatography (HPLC). Both acetone/water and continuous soxhlet extraction with methanol were equally effective in extracting ellagic acid from strawberries. In raspberries, the amount of ellagic acid liberated by the two extraction methods was not significantly different after hydrolysis with trifluoroacetic acid. The extracts were stable over 90 days at -20, 4, and 25°C. Analysis showed that every food sample tested contained ellagic acid, but only the amounts present in strawberries (630 pg), raspberries (1500 pg), blackberries ( 1500 pg), walnuts (590 rg), pecans (330 pg), and cranberries (120 fig ellagic acid/g dry wt) were within the calibration range of the assay. In strawberries, 95.7% of the ellagic acid was found in the pulp while 4.3% was contained in the seeds. The seeds of raspberries contained 87.8% of the ellagic acid, and 12.2% was present in the pulp. The juice of both fruits contained negligible amounts of ellagic acid. 0 1989 Academic PES, inc.
Quantitation of ellagic acid in blackberries
Hemijska industrija, 2014
The objective of this study was to evaluate the content of ellagic acid in blackberries, as well as in the juice of different blackberry cultivars obtained using a specific technology. The analysis of the ellagic acid content in the test samples was performed using the high-pressure liquid chromatography with diode-array detection (HPLC-DAD). Results showed considerable variations in ellagic acid content in the test blackberry fruit samples, the highest being determined in the fruit of the blackberry- raspberry hybrid cv. 'Tayberry' (54.794 mg/100 g fresh weight), and the lowest in blackberry cv. 'Cacanska Bestrna' (1.852 mg/100 g fresh weight). The ellagic acid content in the 'Tayberry' juice produced using the specific technology was very high and almost identical to that in fruits.
Extraction and analysis of ellagic acid from novel complex sources
Chemical Papers, 2008
Ellagic acid (EA) was quantified by reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with photodiode array detection (DAD) in five fine-powdered plants collected from the semiarid region of Mexico. Samples analysed included Jatropha dioica branches (Dragon's blood), Euphorbia antisyphyllitica branches (Candelilla), Turnera diffusa Willd leaves (Damiana), Flourensia cernua leaves (hojasén) and Punica granatum husk (pomegranate) at two maturity stages ("turning" or intermediate and maturated fruit, considered as positive controls). The results demonstrated high EA concentrations in all tested samples which are novel sources of this natural antioxidant. The method developed for the EA analysis is fast and it showed an excellent linearity range, repeatability, intra-and inter-day precision and accuracy with respect to the methods reported for the EA analysis.
UV Method Development and Validation of Ellagic Acid for its Rapid Quantitative Estimation
2019
Development and validation of a simple UV- Spectroscopy method was done for the quantitative analysis of Ellagic Acid (EA). The stock solution of 50μg/ml was prepared and scanned, for which absorption maxima was found to be 277nm. Further dilutions to different concentrations (1-5μg/ml) were prepared and analyzed at 277nm. The method so developed was validated as per ICH guidelines for: linearity, robustness, precision, accuracy, limit of detection and quantification. The Lambert- Beer’s law is followed in the range (1-5μg/ml) with correlation coefficient value 0.9994. It was observed that the method is precise and accurate for EA analysis with good recovery percent of 94.47% to 106.83%. The method developed was further employed for determining the entrapment efficiency of ellagic acid and its release from its nanoparticle dosage form. The method may be utilized for determining the concentration of EA when present as formulation and in combination with other drugs.
Antioxidant Assay-Guided Purification and LC Determination of Ellagic Acid in Pomegranate Peel
Journal of Chromatographic Science, 2010
On the basis of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay-guided purification, ellagic acid was isolated from the methanol extract of pomegranate fruit peel by liquid-liquid extraction and chromatographic techniques. A reversed-phase high-performance liquid chromatography was described for determination of ellagic acid in pomegranate fruit peel extract. The method involved the use of a TSK-gel ODS-80Tm column with a mixture of 2% aqueous acetic acid and methanol (gradient elution mode: 0-15 min, 40-60% v/v methanol and 15-20 min, 60% v/v methanol) as the mobile phase and detection at 254 nm. The parameters of linearity, repeatability, reproducibility, accuracy, and specificity of the method were evaluated. The recovery of the method was 98.5% and linearity (r 2 > 0.9995) was obtained for ellagic acid. A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 5%) were also achieved. The limits of detection and quantification were 1.00 and 2.50 µg/mL, respectively. The solvent for extraction of ellagic acid from pomegranate fruit peel was examined in order to maximize the ellagic acid content of the extract. A solution of 10% v/v water in methanol was capable of increasing the ellagic acid content in the extract up to 7.66% w/w. The ellagic acid content and antioxidant activity of the ethyl acetate fraction separated from the crude extract using water and ethyl acetate partition was higher than that of the crude extract. Experimental Plant material Pomegranate fruits were collected from Mengzhi pomegranate garden (Yunnan, China). The fruit peels were dried at 50-60°C in a hot air oven for 24 h and were ground to coarse powder using a grinder. Chemicals and reagents Standard ellagic acid and DPPH were purchased from Fluka (Buchs, Switzerland). Quercetin, kaempferol, and β-carotene were purchased from Sigma (Buchs, Switzerland). HPLC-grade and analytical-grade methanol was purchased from Labscan Asia (Bangkok, Thailand). Acetic acid was from J.T. Baker (Phillipsburg, NJ). Water was purified in a Millipore Milli-Q system (Bedford, MA).
Ellagic Acid Content in Fruits of Selected Strawberry Cultivars
POLISH JOURNAL OF NATURAL SCIENCES, 2011
Ellagic acid is a biologically active compound, regarded as preventive against various diseases and demonstrates anticarcinogenic and antioxidant effects. In Poland, one of the five largest strawberry producers, the supply of these fruits is significant; therefore exploration of this subject and dissemination of the knowledge concerning their nutritional values and health-related properties is important. The aim of the study was to determine the content of ellagic acid in the most popular, selected strawberry cultivars in Poland. Fruits of the strawberry cultivars under examination differed significantly in ellagic acid content. The content of the analysed component ranged from 452.5 mg kg-1 fresh weight (4,575.6 mg kg-1 dry matter) in fruits of the 'Heros' cultivar to 1,193.8 mg kg-1 fresh weight (14,215.0 mg kg-1 d.m.) in fruits of the 'Camarosa' cultivar. The highly diversified content of ellagic acid in fruits of individual cultivars, grown in similar soil and weather conditions and subjected to the same agricultural treatments, indicates that the content of the analysed phenolic acid depends not only on the fruit species, but also on the cultivar.
Current Topics in Nutraceutical Research, 2020
A sensitive and specific liquid chromatography tandem mass spectrometric method was developed and validated for the simultaneous determination of rosuvastatin (ROS) and N-desmethyl rosuvastatin (NOR-ROS) in human plasma using deuterium-labeled internal standards. The plasma samples were prepared using liquid-liquid extraction with diethyl ether. Chromatographic separation was accomplished on an Xterra MS C 18 column. The mobile phase consisted of a gradient mixture of 15 mmol/L ammonium acetate in water and in methanol, maintained at a flow rate of 0.4 mL/min. Mass spectrometric detection was carried out in negative electrospray ionization mode and monitored by quantification and qualification transitions for each analyte. Using 300 mL plasma samples, the lower limits of quantification of ROS and NOR-ROS were 0.05 and 0.02 mg/L respectively. The linearity of ROS and NOR-ROS ranged from 0.05 to 42 and 0.02 to 14 mg/L respectively. The relative standard deviations of ROS and NOR-ROS were <13 and 9%, respectively, while the deviations from expected values were within À4.7-9.8 and À5.2-4.6%, respectively. The present method offered high sensitivity and was successfully applied to a 24 h pharmacokinetic study of ROS and NOR-ROS in healthy subjects receiving a single dose of 10 mg ROS.
2017
Background and Aim: The peels of six Iranian pomegranates ( Punica granatum L.) cultivars, as a traditional medicine, were treated with a new miniaturized matrix solid-phase dispersion (MSPD) method for the HPLC determination of ellagic acid (EA). Materials and Methods: In the proposed method, only 10mg of the sample powder was ground and blended with an equal amount of C18 sorbent in an agate mortar. The use of the agate mortar with smooth surface facilitated the sample transfer into a cartridge and reduced the required amount of sample and sorbent. Micro volumes of dichloromethane, n-hexane and methanol were used as modifier, washing and elution solvents, respectively. The eluate was injected into an HPLC-UV system for the analysis. Results: Several factors such as the type and amount of dispersing sorbent, modifier, washing solvent and eluent were carefully studied and optimized. Six replicated analyses at the optimized conditions resulted in a recovery of 96.7% and a relative st...
Food Chemistry, 2011
The voltammetric behaviour of ellagic acid (EA) is investigated by cyclic, differential pulse and squarewave voltammetry (CV, DPV and SWV, respectively). Based on the anodic oxidation peak at approximately 0.42 V in acetic/acetate buffer (pH 5.5) a robust and a highly reliable square-wave voltammetric method is presented for the determination of EA. The oxidation peak current was linearly dependent on the concentration of EA in the range of 1.0 Â 10 À7 -1.5 Â 10 À6 mol/L (r = 0.9997), with a detection limit of 1.0 Â 10 À8 mol/L (S/N = 3) and a quantification limit of 3.4 Â 10 À8 mol/L (S/N = 10), good reproducibility and a satisfactory level of selectivity towards others polyphenols. The proposed method was applied to the determination of free and total EA in fruits, nuts and juices with good analytical results being obtained.
Hydrolytic Properties of Ellagic Acid in I
2012
Pomegranate and pomegranate juices (PJs) have taken great attention for their health benefits in the last years. As there is an increasing concern about potential health benefits of ellagic acid, it is of great interest to evaluate alterations in ellagic acid concentration of commercial PJs. The purpose of this study is to analyze total phenolic, free and total ellagic acid content of six commercial PJs sold in Turkish markets using HPLC. The results showed that some commercial PJs had markedly high total phenolic and ellagic acid content. Total phenolic substances of commercial PJs range from 796.71 to 4608.91 mg GAE/l. Free amount of ellagic acid in commercial PJs range from 27.64 to 111.78 mg/l. Samples are hydrolyzed with concentrated HCl at 93C for 2 and 24 hour and influences of temperature and time parameters on hydrolization were investigated. Thermal processing for pasteurization increased ellagic acid via ellagitannins hydrolysis. Keywords—Ellagic acid, ellagitannin, pomeg...