Green Synthesis, Characterization of Silver Nanoparticles of a Marine Red Alga Spyridia Fusiformis and Their Antibacterial Activity (original) (raw)
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International Journal of Pharmacy and Pharmaceutical Sciences, 2017
Objective: In the present system, the green synthesis of silver nanoparticles using marine the red alga Spyridia fusiformis and antibacterial activity was carried out. Methods: The seaweed extract was used for the synthesis of AgNPs Results: The UV-visible spectroscopy revealed surface plasmon resonance at 450 nm. The FT-IR measurements showed the possible functional groups responsible for the formation of nanoparticles. The X-ray diffraction analysis showed that the particles were crystalline in nature. TEM micrograph has shown the formation of silver nanoparticles with the size in the range of 5-50 nm. The silver nanoparticles synthesized from the S. fusiformis showed higher activity and proved their efficacy in controlling the pathogenic bacterial strains. The nanoparticles showed highest inhibition activity on K. pneumaniae and S. aureus up to 26 and 24±0.01 mm at 100 μg/ml of nanoparticles. at room temperature. The silver nanoparticles were characterized by using UV-Visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscope and X-ray diffraction (XRD) techniques. The antibacterial activity of biosynthesized silver nanoparticles was carried out by disc diffusion method against pathogenic bacteria. Conclusion: The synthesised AgNPs have shown the best antibacterial activity against human pathogens
Green Synthesis of Silver nanoparticles Using Marine Algae Extract and Their Antibacterial Activity
2018
In this study, we used an environmentally friendly chemistry to synthesize nanoparticles (AgNPs) embedded biological marine extracts (BME) from four species of marine algae. Twelve extracts from four species of seaweeds (U. fasciata , Grateloupia sp., P. capillacea and C. mediterranea) were used for synthesis AgNPs/BME, antibacterial properties were investigated and hence anti-biofilm activity against indicator strains and bacterial community using well-cut diffusion technique and pouring technique. The positive records of antibacterial activity ranged between 2.4 and 23.6 AU. Occasionally, as well as, SUDW showed high AU (6.6) against bacterial community in water from both harbors. Aqueous extract of Ulva fasciata was selected highly efficient, among all algae species screened for the green synthesis of AgNPs/BME. The antibacterial activity of AgNPs/UE against both gram-positive and gram-negative bacteria(Staphylococcus aureus, Streptococcus faecalis, Pseudomonas aureogenosa, Esche...
Biological or green synthesis of nanoparticles is a relatively new emerging field of nanotechnology which are cheaper, less-labor and eco-friendly benefits over chemical and physical processes of synthesis. In the present work, three different seaweeds species (Caulerpa prolifera, Sargassum acinarium and Gigartinia teedi) aqueous extracts were compared as bioreductants for the synthesis of nanostructure silver particles (Ag-NPs). Structural; morphological and optical properties of the synthesized nanoparticles have been characterized systematically by using ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). UV–visible spectrometry study revealed different surface plasmon resonance according to species of seaweeds and incubation time. The nanoparticles were crystalline in nature confirmed by the XRD pattern. A TEM images showed that the particles are spherical in shape with size ranging from 2 to 22nm according to the species. FT-IR spectroscopy confirmed the presence of bio-components in the seaweeds extracts which were responsible for the nanoparticles synthesis. The synthesized nanoparticles have highly effective antimicrobial activity against eight tested pathogenic microbial strains compared to their corresponding seaweed extracts, silver nitrate and ampicillin as controls. Results of the present study recommended green synthesis of Ag-NPs from seaweeds to play a vital role in future for industrial and/or therapeutic applications.
Arabian Journal of Chemistry, 2019
This work presents a simple method for the green synthesis of silver nanoparticles (AgNPs) using as reducing and stabilizing agent a polysaccharide extracted from red algae Gracilaria birdiae present in the coast of Piauı´. The AgNPS were prepared using three polysaccharide concentrations (0.02, 0.03 and 0.05% v/v) and two pHs (10 and 11) at stirring for 30 min at 90°C. The formation of silver nanoparticles was monitored by measurements of UV-vis and FTIR and characterized by size and zeta potential measurements using DLS and morphologically by TEM. The UV-vis absorption spectrum showed the surface plasmon peak at 410 nm, which is characteristic peak of silver nanoparticles. The functional biomolecules present in the polysaccharide and the interaction between the nanoparticles were identified by the Fourier transform infrared spectroscopy (FTIR) analysis. The stability of the synthesized silver nanoparticles was analyzed during four months and no significant agglomeration was observed. The hydrodynamic diameter of the AgNPs varied between 20.2 nm and 94.9 nm. The AgNPs were tested for antimicrobial activity using Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) and all
We present the synthesis and antibacterial activity of silver nanoparticles using Caulerpa racemosa, a marine algae. Fresh C. racemosa was collected from the Gulf of Mannar, Southeast coast of India. The seaweed extract was used for the synthesis of AgNO3 at room temperature. UV–visible spectrometry study revealed surface plasmon resonance at 413 nm. The characterization of silver nanoparticle was carried out using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and transmission electron microscope (TEM). FT-IR measurements revealed the possible functional groups responsible for reduction and stabilization of the nanoparticles. X-ray diffraction analysis showed that the particles were crystalline in nature with face-centered cubic geometry. TEM micrograph has shown the formation of silver nanoparticles with the size in the range of 5–25 nm. The synthesized AgNPs have shown the best antibacterial activity against human pathogens such as Staphylococcus aureus and Proteus mirabilis. The above eco-friendly synthesis procedure of AgNPs could be easily scaled up in future for the industrial and therapeutic needs.
Enhanced bactericidal effect of silver nanoparticles synthesized using marine brown macro algae
In the present investigation silver nanoparticles of spherical shape was synthesized biologically by rapid biological drip method using marine brown macro algae, Sargassum wightii (S. Wightii). Seaweed extract act as a reducing agent for silver nitrate. Silver nanoparticles have been characterized by UV-Visible spectrophotometer, Fourier-Transform Infrared spectroscopy (FTIR), X-ray diffractometer (XRD) and High Resolution Transmission Electron Microscope (HR-TEM). Antibacterial activity of these nanoparticles was carried out by antibiotic disc diffusion method against pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae. Seaweed S. wightii mediated silver nanoparticles were found to be effective against these pathogens than silver nitrate.
In the present study, we report the aqueous extract of Pithophora oedogonia to produce silver nanoparticles (AgNPs) by reduction of silver nitrate. It was noted that synthesis process was considerably rapid and silver nanoparticles were generated within few minutes of silver ions coming in contact with the algal extract. A peak at 445 nm corresponding to the plasmon absorbance of AgNPs was noted in the UV–vis spectrum of the aqueous medium that contained silver ions. Scanning electron microscopic (SEM) and dynamic light scattering analysis of colloidal AgNPs indicated the size of 34.03 nm. Energy-dispersive X-ray spectroscopy revealed strong signals in the silver region and confirmed of the AgNPs. Fourier transform infrared spectroscopic analysis of the nanoparticles indicated the presence of protein which was regarding a capping agent surrounding the AgNPs. Moreover, the antibacterial activity of synthesized nanoparticles exhibited potential inhibitory activity against seven tested pathogenic bacteria.