Distrubution of Extended Spectrum β-Lactamase and AMPC-β Lactamase Among Bacteria Isolated From Urine Samples (original) (raw)
Related papers
Malaysian Society for Microbiology, 2018
Aims: This study aimed to determine the prevalence of pathogens in urinary tract and their antimicrobial susceptibilities, based on extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase production in Bangladesh. Methodology and results: The prevalence of pathogenic microorganisms in urinary tract and their antimicrobial resistance patterns were identified in 200 isolates from patients with urinary tract infections. Combined disc diffusion was performed to identify the presence of ESBL-producing strains. Moreover, disc approximation assay, disc potentiation test and double disc synergy test were performed to determine the presence of AmpC beta-lactamase producing bacterial strains. This study demonstrated a higher prevalence of UTIs in females (83.5%) than in males (16.5%). The most common pathogen was found Escherichia coli (44.5%), followed by Enterococcus fecalis (24%), Klebsiella pneumoniae (7.5%), Staphylococcus aureus (6%), Pseudomonas aeruginosa (5.5%) and Staphylococcus saprophyticus (4.5%). ESBL and AmpC beta-lactamase production occurred more frequently in E. coli (25.84%) and P. aeruginosa (100%) respectively. Conclusion, significance and impact of study: The result of this study would provide physicians with important information which help them to make a judicious choice of antibiotics for therapeutic purposes. However, it is emphasized that continuous surveillance of antibiogram of medically important organisms causing UTI is necessary for adopting a rational antibiotic policy in the country.
Malaysian Society of Microbiology, 2019
Aims: This study aimed to determine the prevalence of pathogens in urinary tract and their antimicrobial susceptibilities, based on extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase production in Bangladesh. Methodology and results: The prevalence of pathogenic microorganisms in urinary tract and their antimicrobial resistance patterns were identified in 200 isolates from patients with urinary tract infections. Combined disc diffusion was performed to identify the presence of ESBL-producing strains. Moreover, disc approximation assay, disc potentiation test and double disc synergy test were performed to determine the presence of AmpC beta-lactamase producing bacterial strains. This study demonstrated a higher prevalence of UTIs in females (83.5%) than in males (16.5%). The most common pathogen was found Escherichia coli (44.5%), followed by Enterococcus fecalis (24%), Klebsiella pneumoniae (7.5%), Staphylococcus aureus (6%), Pseudomonas aeruginosa (5.5%) and Staphylococcus saprophyticus (4.5%). ESBL and AmpC beta-lactamase production occurred more frequently in E. coli (25.84%) and P. aeruginosa (100%) respectively. Conclusion, significance and impact of study: The result of this study would provide physicians with important information which help them to make a judicious choice of antibiotics for therapeutic purposes. However, it is emphasized that continuous surveillance of antibiogram of medically important organisms causing UTI is necessary for adopting a rational antibiotic policy in the country.
Scientific Reports
Nowadays, increasing extended-spectrum β-lactamase (ESBL)-producing bacteria have become a global concern because of inducing resistance toward most of the antimicrobial classes and making the treatment difficult. In order to achieve an appropriate treatment option, identification of the prevalent species which generate ESBL as well as their antibiotic susceptibility pattern is essential worldwide. Hence, this study aimed to investigate the prevalence of ESBL-producing bacteria and assess their drug susceptibility in Fardis Town, Iran. A total of 21,604 urine samples collected from patients suspected to have urinary tract infection (UTI) were processed in the current study. The antimicrobial susceptibility of the isolates was tested by the disk diffusion method. The ESBL producing bacteria were determined by Double Disc Synergy Test (DDST) procedure. Bacterial growth was detected in 1408 (6.52%) cases. The most common bacterial strains causing UTI were found E. coli (72.16%), follow...
International Journal of Research in Pharmaceutical Sciences
Extended Spectrum B-Lactamase producing bacteria has become a worldwide problem and made the treatment very much complicated due to their resistance ability against the wide number of antimicrobial drug. This study was undertaken to measure the emergence of ESBL producing bacteria in urine collected from the patient Chhattisgarh Institute of Medical Science, Bilaspur, C.G. A total of 141 Eterobactericeae groups isolates and identified. A Total of 81 ESBL producing uropathogens were identified. The most prevalence of ESBL producing bacteria was E. coli 59(41.84%) followed by Klebsiella species 12(8.51%), and Enterobacter species were 06(4.26%). ESBL producers are confirmed by the Phenotyping confirmatory disc diffusion test. Antimicrobial susceptibility testing by Kirby-Bauer method showed most resistance were ampicillin (88.9%), Ciprofloxacin (69.1%), amoxycillin clavulanic acid (76.5%) resistance to ESBL producers and least resistant to imipenem 15(18.5%). We are confident that thi...
2018
Introduction: Occurrence of Extended Spectrum beta-lactamase (ESBLs) producing bacteria have presented impediment in treatment choices for urinary tract infections. ESBLs embody a major cluster of lactamases accountable for resistance to novel generations of s-lactam drugs worldwide. The study determined prevalence of ESBL organisms in urine isolates and susceptibility patterns to 13 antibacterial agents. Materials and methods: Two hundred samples were cultured on blood agar, MacConkey agar and incubated at 37°C utmost 48 hours. Isolates identified based on standard bacteriological culture and biochemical characteristics. Drug susceptibility centered on Clinical Laboratory Standard Institute recommended and WHO modified Kirby- Bauer disc diffusion methods. Isolates with reduced susceptibility to Ceftazidime were considered to be possible ESBL producers. Phenotypically confirmed ESBL required use of Ceftazidime in combination with Clavulanic acid. A five milimeter increase zone diame...
Journal of Gandaki Medical College-Nepal
Introduction: Extended spectrum β-lactamase producing gram negative bacilli has emerged and spread worldwide as an important cause of urinary tract infections and indicates a major threat. The study aimed to determine the prevalence of multidrug resistant and extended spectrum beta-lactamase producing gram negative bacterial uropathogens among females in a tertiary level hospital. Methods: A hospital-based cross-sectional study was conducted in Pokhara Academy of Health Sciences, Pokhara from February to November, 2019. A total number of 301 midstream urine samples were collected and examined using MacConkey agar and blood agar medium. Antimicrobial susceptibility testing was done by Kirby Bauer disk diffusion method on Mueller Hinton agar using Clinical and Laboratory Standards Institute guidelines. Results: Out of 301 mid-stream urine samples, 99(33%) sample showed significant bacterial growth. Among them, 78(79%) were gram negative bacteria. Escherichia coli were the predominant ...
Tribhuvan University Journal of Microbiology
Objectives: The study was aimed to determine the prevalence of Extended Spectrum Beta Lactamase (ESBL) producing Gram negative pathogens from urine samples along with their antimicrobial resistance. Methods: This cross-sectional study was conducted from December 2015 to May 2016 at Everest Hospital, Kathmandu. Mid-stream urine samples were collected and processed for culture by standard loop streak method. Identified bacterial isolates were tested for Antibiotic Susceptibility by modified Kirby Bauer disc diffusion method and, were subjected to ESBL screening by using 30µg cefotaxime and ceftazidime. ESBL production was confirmed by combination disc method. Results: Of the three hundred urine samples, 22.7% (67/300) showed significant growth. Four different bacterial species were identified. Among the isolates, E. coli was the most common pathogen (71.64%) followed by Klebsiella pneumoniae (14.92%), Pseudomonas spp (8.95%) and Acinetobacter spp (4.48%). Altogether 92.54% (n=62) isol...
EPIDEMIOLOGICAL STUDIES ON VARYING EXTENDED-SPECTRUM β-LACTAMASES PRODUCING UROPATHOGENIC BACTERIA
International journal of Pharmacy and Pharmaceutical Sciences, 2014
Objective: Urinary tract infection (UTI) is the most common serious infection during infancy, adult male and female, as well as at the time of pregnancy. UTI is also the most common nosocomial infection in many hospitals and accounts for approximately 35% of all hospital acquired infections. Hence the present study was aimed to screen the presence of multi-drug resistant bacterial pathogens among the urine samples collected from in and out patients of multi-speciality hospital. Methods: Standard microbiological laboratory protocols were followed and about 152 samples were processed and screened. Among them, 49 reported positive for the presence of urinary bacterial pathogens. Results: Among 49 isolates, Escherichia coli registered its prevalence in about 44 samples followed by Klebsiella spp. (4) and Pseudomones spp. (1). The gender wise distribution was found to be more among female patients (42%) than male patients (24%). The age wise distribution of infection among male and female patients was also noticed. Further, the multi-drug resistance of the isolates was done by using 8 antibiotics. Conclusion: All the isolates exhibited the multiple antibiotic resistance and the isolates showed 27 different antibiotic resistance patterns. This confirms the prevalence of ESBL producers among the urinary pathogens.
Science World Journal, 2020
Urinary tract infection is a common health problem in both community and nosocomial setting. Microbiological analysis of mid-stream urine (MSU) of out-patients were carried out using standard microbiological technique. The presence of glucose, protein, ketone, leucocyte, bilirubin and nitrite were found out using dip sticks. The phenotypic detection of extended spectrum betalactamase (ESβL) and antibiogram of isolates were determined by disc diffusion method. Of the 150 MSU samples from out-patients, 34.7% had significant bacteriuria (SBU), while 65.3% showed no significant bacteriuria. There was no statistically significant relationship between the occurrences of SBU among subjects based on ages (p=0.567), marital status (p=0.063), educational levels (p=0.789) and occupation (p=0.134) whereas based on gender, there was statistically significant difference at p ˂ 0.05. Sixty (40.0%) of MSU samples had leucocytes, 29.3% contained nitrite, 27.3% contained urobilinogen, 28.7% contained...
IP International Journal of Medical Microbiology and Tropical Diseases, 2018
Introduction: Urinary Tract infection (UTI's) is the most commonly encountered infectious disease, affecting both the sexes in all age groups. This leads to consumption of large number of antibiotics and development of resistant strains leading to complications. Objective: The study was carried out to know the distribution pattern and to evaluate the sensitivity of ESBL producing urinary isolates from patients with UTI. Materials and Methods: A cross sectional study was conducted at Owaisi Hospital and Research Centre for 6months. A total of 392 samples were obtained from suspected UTI patients. Clean catch mid stream urine samples obtained were subjected to microscopy, culture and antibiotic sensitivity testing as per CLSI guidelines. Results: From the total of 392 samples, 200 samples i.e. 51% samples were culture positive. Among the culture positive cases, 69% were females, 35% were males. 38.8% of Esch.coli and 25% Klebsiella were predominant ESBL producers. Conclusion: This study revealed that majority of Gram negative urinary isolates are ESBL producers, and multidrug resistant. Thus strict policy with guidelines for antibiotic susceptibility testing and prescription should be followed to prevent further emergence of resistant strains and treatment failure.