Aerobic dehalogenation activities of two petroleum degrading bacteria (original) (raw)
Related papers
Isolation and identification of petroleum degrading bacteria
Current Opinion in Biotechnology, 2011
Oil spillage has become a global environmental problem. Natural bioremediation is the only ecofriendly solution to resist its devastating environmental and economic damage damage. In this investigation, petroleum tolerant and degrading bacteria were isolated from different oil-contaminated soil and water samples. Bushnell Haas media supplemented with petrol, kerosene, and diesel as sole carbon sources was used for isolation of bacteria capable of degrading these petroleum fractionates. From three soil samples and two water samples, a total of nine bacterial strains were isolated capable of degrading petrol, kerosene and diesel with varying tolerance capacities. The isolates were identified by using standard biochemical tests and morphological studies, and it was determined that these strains belong to six bacterial genera e.g., Staphylococcus, Micrococcus, Streptococcus, Bacillus, Klebsiella and Corynebacterium. The isolated Staphylococcus spp. were found to be the most tolerant isolate withstanding as high as 7% petroleum. The others also exhibit tolerance to varying concentrations of petroleum. All these isolates were able to degrade petroleum completely and produced CO 2 within 7 days, with a few exceptions for Bacillus sp. and Klebsiella sp. which required 15 days for complete degradation of kerosene. These isolates seemed to have potential for bioremediation of oil contaminated soil and water.
Journal of Taibah University for Science, 2018
This study demonstrated the potential for reductive dechlorination of aryl halides in a sedimentary environment by naturally occurring bacteria. A laboratory sediment microcosm and highly enriched, stable dechlorinating cultures were established using hydrogen as electron donor and 2,3-dichlorophenol (2,3DCP), monochlorophenol (MCP) and dichlorobenzene (DCB) as electron acceptors. 16S rRNA gene sequencing of dominant DGGE bands assigned detected phylotypes in chlorophenols (CPs) amended sediment microcosms to the genera Anaerospora, Pseudomonas, Desulfitobacterium, Clostridium, Mycobacterium, and Peptoclostridium, beside uncultured marine Bacterioplankton, Halochromatium and Sedimentibacter. Chlorobenzene (CB) amended sediments showed the same community in addition to Bacillus sp. Major operational taxonomic units (OTUs) in CPs enrichment cultures were assigned to Anaerospora hongkongensis, Pseudomonas stutzeri, Pseudomonas pseudoalcaligenes, Clostridium sp., Desulfitobacterium dichloroeliminans, beside unidentified marine bacterioplankton. Enrichment on DCB resulted in predominance of the same populations in addition to Peptoclostridium sp. and Dehalococcoides mccartyi. Dechlorination in enrichment cultures was mainly assigned to Desulfitobacterium and Dehalococcoides, which are both known for their ability to couple dechlorination to growth in a halorespiration way. Enrichment cultures containing Desulfitobacterium showed ortho dechlorination activity of 2,3DCP to 2 chlorophenol (2CP) and 3 chlorophenol (3CP). These results provided compelling evidence that sedimentary environments in the vicinity of oil refineries do harbor dehalorespiring bacteria capable of reductive dechlorination of various aryl halides pollutants.
International Journal of Environmental Research, 2019
Petroleum hydrocarbons are highly toxic to plants, animals, and humans and are carcinogenic effects to plants, animals, and humans. The present study is focused to enhance the degradation of petroleum hydrocarbons using potential indigenous bacterial isolates. Bacteria were isolated from different petroleum oil-contaminated sites and characterized. Bacterial growth was evaluated under different physico-chemical parameters. The petroleum hydrocarbon degradation potentiality was assessed using GC-MS analysis. Forty-nine bacterial isolates were screened; only three isolates (ALK-14, ALK-16, and ALK-23) have good potential to degrade petroleum hydrocarbons and were identified as Alcaligenes species ALK-14, Bacillus methylotrophicus ALK-16, and Enterobacter species ALK-23, respectively, on the basis of morphology, biochemical characterization, and 16S rRNA sequencing. Optimum growth of Alcaligenes species ALK-14, Bacillus methylotrophicus ALK-16, and Enterobacter species ALK-23 occurred at pH 7, 6, and 6, respectively. Petroleum oil concentration found suitable for growth of selected bacterial isolates Alcaligenes species ALK-14, Bacillus methylotrophicus ALK-16, and Enterobacter species ALK-23 were 4%, 8%, and 4% (v/v), respectively. Among different nitrogen sources, ammonium nitrate was found suitable source for the maximum growth of Alcaligenes species ALK-14, Bacillus methylotrophicus ALK-16 at the concentration of 0.15 and 0.2%, respectively, and sodium nitrate for Enterobacter species ALK-23 at the concentration of 0.2%. All bacterial isolates showed maximum growth at 30 °C temperature. Synergistic effect on the growth of bacterial isolates under favourable conditions increased up to 11-28%. GC-MS analysis indicated that the hydrocarbon compounds in the range of C 20-C 44 were present in petrol. Maximum hydrocarbon degradation by Alcaligenes species ALK-14, Bacillus methylotrophicus ALK-16, and Enterobacter species ALK-23 was 11.65%, 8.11%, and 5.59%, respectively. The degradation of docosane 11-decyl, hexatriacontane, and eicosane by Alcaligenes species ALK-14 was 100%, 60%, and 48.8%, respectively. The degradation of docosane 11-decyl by, Bacillus methylotrophicus ALK-16, was 80%. Degradation of hexatriacontane by Enterobacter species ALK-23 was 31%. Further understanding of the metabolic processes of these organisms on crude oil hydrocarbons degradation will increase possibilities to develop strategies for removing crude oil pollutants from oil-impacted environments. Article Highlights • Bacterial isolates Alcaligenes species ALK-14 degraded docosane 11-decyl, hexatriacontane and eicosane by Alcaligenes species ALK-14 was 100%, 60% and 48.8 % respectively and the obtained degradation pattern was 11-decyl > hexatriacontane > eicosane. • Under optimized conditions, the parameters have showed synergistic effect therefore bacterial growth was enhanced up to 11-28% which is directly proportional to the hydrocarbon degradation. • Eighty percent degradation of docosane 11-decyl was achieved by Bacillus methylotrophicus ALK-16.
Isolation and Characterization of a Hydrocarbon-Degrading Bacterial Strain
2010
The isolation of hydrocarbon-degrading bacteria in topsoil and subsoil samples of selected mechanic workshops located in the Benin metropolis was carried out. The highest and lowest bacterial counts in topsoil samples were found out to be 2.94 x 10 6 CFU/g and 2.39 x 10 6 CFU/g in CENTRAL road and OGBELAKA road automobile workshops respectively. The highest and lowest bacterial counts in subsoil samples were found out to be 1.14 x 10 6 CFU/g and 1.09 x 10 6 CFU/g in OGBELAKA r oad and CENTRAL road automobile workshops respectively. The bacterial species isolated were Bacillus spp., Pseudomonas spp., Staphylococcus spp. and Streptococcus spp. The isolates were also subjected to hydrocarbon degradation/utilization test where it was observed that Pseudomonas spp utilized the hydrocarbon in the medium more efficiently than the other isolates. The isolates from this study are thus recommended as bacterial species for possible use in bioremediation of hydrocarbons. © JASEM
Bacterial Metabolism of Petroleum Hydrocarbons
Deliberate and accidental releases of petroleum hydrocarbons (PHCs) have particular concern in the environment. PHC components belong to the family of carcinogens and neurotoxic organic pollutants. At present accepted disposal methods of incineration or burial insecure landfills can become prohibitively expensive when contaminants are released in large quantities. Physical and chemical processes used to remove PHCs from contaminated environments are expensive and have limited effectiveness. Bioremediation is the potentially promising technology for the treatment of these contaminated sites. Since it is cost-effective and leads to complete mineralization of contaminants. Bioremediation functions basically on biodegradation, which may refer to complete mineralization of organic contaminants into carbon dioxide, water, inorganic compounds, and cell protein or transformation of complex toxic organic contaminants to the simple and environmentally innocuous forms by biological agents like microorganisms. Many indigenous microorganisms in soil and water environments are capable of degrading hydrocarbon contaminants. This chapter provides an overview of bacterial metabolism of petroleum hydrocarbon PHCs in the environment.
Oil spillage has become a global environmental problem. Natural bioremediation is the only ecofriendly solution to resist its devastating environmental and economic damage damage. In this investigation, petroleum tolerant and degrading bacteria were isolated from different oil-contaminated soil and water samples. Bushnell Haas media supplemented with petrol, kerosene, and diesel as sole carbon sources was used for isolation of bacteria capable of degrading these petroleum fractionates. From three soil samples and two water samples, a total of nine bacterial strains were isolated capable of degrading petrol, kerosene and diesel with varying tolerance capacities. The isolates were identified by using standard biochemical tests and morphological studies, and it was determined that these strains belong to six bacterial genera e.g., Staphylococcus, Micrococcus, Streptococcus, Bacillus, Klebsiella and Corynebacterium. The isolated Staphylococcus spp. were found to be the most tolerant isolate withstanding as high as 7% petroleum. The others also exhibit tolerance to varying concentrations of petroleum. All these isolates were able to degrade petroleum completely and produced CO 2 within 7 days, with a few exceptions for Bacillus sp. and Klebsiella sp. which required 15 days for complete degradation of kerosene. These isolates seemed to have potential for bioremediation of oil contaminated soil and water.
Bioresource Technology, 2017
Intrinsic biodegradation potential of bacteria from petroleum refinery waste was investigated through isolation of cultivable strains and their characterization. Pseudomonas and Bacillus spp. populated the normal cultivable taxa while prolonged enrichment with hydrocarbons and crude oil yielded hydrocarbonoclastic bacteria of genera Burkholderia, Enterobacter, Kocuria, Pandoraea, etc. Strains isolated through enrichment showed assemblages of superior metabolic properties: utilization of aliphatic (C6-C22) and polyaromatic compounds, anaerobic growth with multiple terminal electron acceptors and higher biosurfactant production. Biodegradation of dodecane was studied thoroughly by GC-MS along with detection of gene encoding alkane hydroxylase (alkB). Microcosms bioaugmented with Enterobacter, Pandoraea and Burkholderia strains showed efficient biodegradation (98% TPH removal) well fitted in first order kinetic model with low rate constants and decreased half-life. This study proves that catabolically efficient bacteria resides naturally in complex petroleum refinery wastes and those can be useful for bioaugmentation based bioremediation.
Study of the Potential of Two Isolated Microorganisms to Degrade Various Petroleum Fractions
Biosciences Biotechnology Research Asia, 2011
Biodegradation of petroleum by the microorganisms in polluted site has been gaining attention from the environment clean-up point of view. Two isolated organisms Acinetobacter junii CTA 3 and Pseudomonas aeruginosa OCD 1 were tested for their petroleum degrading ability in liquid Bushnell-Hass broth. Both the organisms degraded higher boiling petroleum fractions more efficiently. The strain OCD 1 showed better degrading ability than the strain CTA 3. The highest degradation of 47% and 57% was obtained with diesel after 20 days of incubation by the organism CTA 3 and OCD 1 respectively. Also it was observed that, most of the oils were degraded in first 10 days of incubation for both the isolates.