Double-phase culture system for large scale production of pineapple (original) (raw)

Micropropagation and Growth of in Vitro Pineapple (Ananas Comosus L. Merr) in Iran

2014

Although, several pineapple micropropagation protocols have already been published, significant improvement could be achieved, if the stages of in vitro culture were better defined. Our work concerned several experiments aiming at the mass production of high quality plantlets. Tissue culture experiments were therefore conducted to develop rapid multiplication procedures for Ananas comosus L. (Merr). Terminal buds from suckers were treated with 0.025% (w/v) mercuric chloride for 2 minutes and placed in different media. Explants were transferred to MS medium supplemented with NAA (2 mgl -1 ) and BA ( 0, 1, 2, 3, 4, 5, 6, 7 mgl -1 ) and kept for 2-4 months under 16/8 h photoperiod (40 µmol m -2 s -1 ) and 25 ± 2oC. Results showed that higher multiplication rates for Ananas comosus L. were obtained with BA concentrations of 5 mgl -1 at 3 months. The in vitro proliferated shoots produced roots with maximum frequency (84%) on MS medium without growth regulator at 6 weeks intervals. Using ...

Employment of in Vitro Technology for Large Scale Multiplication of Pineapples (Ananas Comosos)

2004

Experiments were carried out for the micropropagation of pineapples. It is thus possible to produce disease free, uniform propagules at needed quantity and at appropriate time of the year. BAP @ 0.5 mg/L showed better results for the number and length of shoots per explant. IBA and NAA were employed for appropriate root initiation and development and the best media observed containing IBA @ 1.5 mg/L alone. This was followed by field experiments to determine an appropriate media for in situ plant growth in green houses, for acclimatizing the plants.

Studies on Micropropagation of Pineapple (Ananas comosus L

The present study was conducted at the Tissue Culture Laboratory, Horticulture, Research Institute, Agricultural Research Center (ARC), Egypt during the period from December 2013 to March 2016 to investigate the effect of different type Murashige and Skooge (MS), Gamborge (B5) and Woody plant media (WPM) at four salt concentrations (Full, ¾, ½ and ¼) of culture media on micropropagability of pineapple (Ananas comosus L. var. Smooth Cayenne) during establishment stage. Shootlet proliferations were investigated at different concentrations of 6-benzyle amino purine (BAP) and kinitine (Kin) at 0.25, 0.5, 1.0 and 2.0 mg/l for each, during two successive subcultures. Finally, rooting capacity were studied by various concentrations of indole butyric acid (IBA) and indole acetic acid (IAA) at1.0, 2.0 and 3.0 mg/l on media containing activated charcoal. The rooted explants were transferred to greenhouse in peat moss and sand at various amounts 1:1, 1:2 and 2:1, respectively. The culture crowns were successfully disinfecting by Colorex 40% for 20 min with 77.77% survival and 100% free contamination. MS media at full strength was the best culture media showed shootlet number 1.75 shootlet/explant and shootlet length 2.25 cm with 9.25 leaf/shootlets. Among the different concentrations 2.0 mg/l BAP showed highest shoot proliferation of 56 and 42 shoots per explant at the first and second subculture, respectively. The longest shoot (5.5 and 5.5 cm) was produced in the two subcultures by the treatment combination of 0.25 mg/l BAP. The highest numbers of roots were produced by 1.0mg/l IAA were 10.67 roots/shootlet and the tallest length of roots were obtained for explants cultured on MS media containing IAA 3 mg/l. The individual rooted explants derived from plantlets were transferred to poly bags in the green house at mixture media after 7 days hardening in room temperature (28-30°C) and established plantlet was ready for planting.

An Efficient and Cost Effective Protocol for In Vitro Propagation of Pineapple

An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole acetic acid (IAA) and 50 mg/l adenine sulphate as RBC design experiment. Two approaches were taken to reduce the chemical cost of micropropagation media. Analytical grade sucrose was successfully replaced by commercial sugar, completely during proliferation stage and up to 66% during rooting stage. Again during the rooting stage, agar based solid media was replaced by liquid media (MS-media). Bio-degradable Coir and Luffa were used as supporting matrix. As sup-porting matrix in rooting media, Luffa was found to be more effective. The clonal fidelity of in vitro raised plantlets was confirmed by RAPD technique. Abstract

Importance of micropropagation in pineapple for disease free plantlets and rapid multiplication

2020

Pineapple (Ananas comosus) is a delicious tropical fruit with a superior flavor and high nutritive value. It is one of the most important commercial fruit crops in the world. Pineapple is propagated by vegetative means from basal, stem slip, and crown suckers with varying degrees of success. The traditional methods of propagation would take 8 years to obtain enough planting materials from one mother plant to plant just only a half hectare. Plant tissue culture methods have been successfully applied to pineapple and it is an efficient method for rapid in vitro clonal propagation. Micro-propagation of pineapple plants also has many advantages, such as, it allows rapid increase of selected varieties, overcomes serious diseases causing considerable production losses of pineapple at the time of planting material preparation disease free plants and increase the multiplication rate of elite genotypes.

Pineapple (Ananas comosus L. Merr) micropropagation in temporary immersion systems

Plant Cell Reports, 1999

A procedure for the mass propagation of pineapple plants (Ananas comosus L. Merr) using a temporary immersion technique is described. This procedure involved three distinct phases in the automated temporary immersion system: shooting, bud differentiation and elongation. To establish this protocol, we used in vitro shoots obtained from established liquid culture as starting materials. Three culture methods (solid, liquid and temporary immersion) were compared. Temporary immersion increased the multiplication rate and fresh and dry weight after 42 days. Conventional micropropagation (liquid medium) and temporary immersion were compared in combination with paclobutrazol. Paclobutrazol promoted the formation of compact bud clusters with limited leaf development. The highest multiplication rate (106) was found when ex-plants were cultured in shooting medium (MS+2.1 mg/l BA+0.3 mg/l NAA) supplemented with 1 mg/l PB for 7 weeks. A 10-l temporary immersion bioreactor was used to test two approaches during elongation stage: reduction of the shoot-formation period or decrease of the initial number of explants. The highest number of competent and uniform plants (191.8 plant/l) was achieved when shoots were cultured for 4 weeks in shooting medium supplemented with PB.