Contractile function recovery in severely injured gastrocnemius muscle of rats treated with either oleic or linoleic acid (original) (raw)

Experimental Physiology, 2016

Abstract

Oleic and linoleic acids control fibroblast proliferation and myogenic differentiation in vitro, however, there was no study in skeletal muscle in vivo. The aim of this study was then to evaluate the effects of either oleic or linoleic acid on muscle content of fibrous tissue (collagen deposition) and contractile function recovery in rat gastrocnemius muscle after being severely injured by laceration. Rats were supplemented with either oleic or linoleic acid during four weeks after laceration (0.44 g per kg body weight per day). Muscle injury led to an increase in oleic/stearic acid and palmitoleic/palmitic acid ratios, suggesting an increase in Δ(9) desaturase activity. Increased fibrous tissue deposition and reduced isotonic and tetanic specific forces and resistance to fatigue were observed in the injured muscle. Supplementation with linoleic acid raised the content of eicosadienoic (20:2, n-6) and arachidonic (20:4, n-6) acids, reduced muscle mass and fiber cross-sectional areas (CSA), increased fibrous tissue deposition and further reduced isotonic and tetanic specific forces and resistance to fatigue induced by laceration. Supplementation with oleic acid raised the content of docosahexaenoic acid (DHA, 22:6, n-3), abolished the increase in fibrous tissue area and the decrease in isotonic and tetanic specific forces and resistance to fatigue induced by muscle injury. We concluded that supplementation with linoleic acid impairs muscle regeneration and increases fibrous tissue deposition, resulting in impaired contractile function recovery. Oleic acid supplementation reduced fibrous tissue deposition and improved contractile function recovery, attenuating the tissue damage caused by muscle injury. This article is protected by copyright. All rights reserved.

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