Relative frequency of human papillomavirus genotypes and related sociodemographic characteristics in women referred to a general hospital in Tehran, 2014- 2015: A cross-sectional study (original) (raw)
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Infectious Agents and Cancer, 2022
Background: Human papilloma virus (HPV) causes the most common sexually-transmitted infection especially among sexually-active individuals. The aim of study was to characterize the molecular characterization of HPV genotypes between 5176 female and male patients. Methods: HPV DNA was extracted from genital swabs of the study participants and amplified by Real Time Polymerase Chain Reaction (PCR). Genotyping was performed for 2525 cases using REALQUALITY RQ-Multi HPV Detection Kit for the identification of 14 high risk (HR) and 2 low risk (LR) HPV genotypes. Demographic figures were analyzed in correlation with virological data statistically. Results: Out of 5176 cases from 7 laboratories, 2727 (53%) were positive for HPV, of which. 2372(87%) women and 355 (13%) men were HPV positive. However, in an intra-gender analysis, positive rate was higher in men (355/637, 55.7%) than in women (2372/4539, 52%; P value 0.007). HPV positive patients were younger than negative individuals. Positive rate was higher among age categories 20-40. Genotyping was performed for 2525 cases. Out of 1219 (48%) patients who contained single genotypes, 566 (22%) and 653 (26%) harboured HR and LR genotypes, respectively. In females and males, 1189 (54%) and 117 (37%) contained multiple genotypes. No substantial associations were found between different age categories and HR/LR and multiple genotypes distribution. Conclusion: The prevalence of HPV infection in both genders was high. However, men had a higher rate of infection. These observations highlighted the necessity for a plan for targeted education to younger population in the society as well as application of infection control measures against HPV infection, especially in terms of general population mass HPV vaccination.
MOLECULAR DIAGNOSIS OF HUMAN PAPILLOMAVIRUS (HPV) GENOTYPES IN TEHRAN CITY.pdf
2017
Background and objective: The human papilloma virus is known as one of the cause’s anogenital cancers, including cervical cancer there is a strong link between presences of different types of human papilloma viruses to the development of genital lesions. HPV16 and HPV18 were defined as most important etiologic agents for cervical dysplasia and carcinoma. The aim of present research was to find genital HPV genome in patients with cervical cancer and control group and to investigate incidence of HPV16, HPV18, HPV31, HPV33, HPV45 and HPV51 types among patients admitted to medical centers. Materials and Methods: Fifty samples of women cervical paraffin-embedded tissue, HPV positive from pathology department of Imam Khomeini (RA) were collected. For this purpose, DNA was extracted and purified using a DNA Extraction Kit (Cinnagen, Tehran, Iran] from 50 patients with cervical cancer attending the Imam Khomaini Hospital (Tehran, Iran) and amplified by PCR. The nucleotide sequences were ana...
Virology Journal, 2010
Background: Some genotypes of human papillomaviruses can infect the genital tract and they are important infectious agents which their oncogenicity is regardable. Thus the aim of this study was to determine the prevalence of various genital human papillomaviruses (HPV) among women being subjected to routine pap smear test in Bushehr city of Iran. Results: Based on the collected data, 11(5.5%) samples were detected positive for HPV DNA and 189(94.5%) samples out of 200 samples were detected negative for HPV DNA. Meanwhile 4(2%) samples detected positive for HPV DNA by PCR were detected positive for HPV by pap smear test as well. On the other hand 5 samples which were detected positive for HPV by pap smear test didn't have HPV DNA after being tested by PCR method. Among the 11 positive samples 7 samples were identified as HPV-16, 3 samples were HPV-18 and one was HPV-53. Conclusion: Regarding the prevalence of highly carcinogen genotypes of HPV in our study determination of genital HPV prevalence among the normal population of women of Bushehr city is recommended.
Asian Pacific journal of cancer prevention: APJCP
Introduction: HPV infection has a prime etiologic role in development and progression of cervical cancer, one of the most frequent forms of cancer among women in developing countries. This study was designed to determine the most prevalent HPV genotypes in women with normal and abnormal cervical cytology in Iran. Materials and Methods: Samples from134 patients, including 127 who attended gynecology clinics and 7 with solid cervical tumors were used. All 127 patients underwent routine Pap tests for cytological evaluation and at the same visit a sample of cervical epithelial cells was obtained by scraping the cervix osteum. In each case HPV infection was primarily evaluated by PCR using GP 5/6 primers and then subtyping was performed in proved infected samples with specific primers for HPV 16, 18, 31, 33, 11 and 6. After cytological evaluation, 50 patients with abnormal Pap tests were categorized as the abnormal group and the remaining 77 patients as the normal group. Results: In the normal group, HPV infection was established in 10 cases (13% infection rate), while 30 HPV positive cases were discovered in the abnormal group (60% infected). The most prevalent genotypes among the infected samples were HPV 16 (76%), HPV18 (12.7%) and HPV11/6 (8.5%). Moreover, all 7 tumor samples were positive for HPV general primers of which, 5 samples were infected with HPV 16, two were co-infected with HPV16,18 and HPV16,31 genotypes and one was infected with HPV 18. Conclusions: Infection with HPV 16 was found to be significantly higher in abnormal group in comparison with normal group (42% vs. 11.6%, P value <0.005), likewise HPV18 genotypes were proved to be more prevalent in abnormal group (8% vs. 0%, P value <0.05). No significant relation between other HPV genotypes and pathologic cervical changes was obtained. According to our study high rates of infection with HPV genotypes in sexually active Iranian women makes molecular investigation for HPV16 and 18 very essential in clinical approaches to patients with proven dysplasia in their screening tests and also for those patients with borderline (i.e. ASCUS) or incongruous pathology reports. Larger studies are required to determine the most appropriate vaccine with highest protection in Iranian women.
Bangladesh Journal of Microbiology, 2010
It is estimated that the incidence of new infections in developing countries, cervical cancer is often the most common cancer in women and may constitute up to 25% of all female cancers 1 . Cervical cancer which is caused by human papillomaviruses (HPVs) are small, nonenveloped viruses of about 55 nm in diameter containing the viral genome as circular double stranded DNA 2 . The 'low risk' HPV types are associated with anogenital warts and condylomas, but do not confer an excess risk for cancer, whereas the 'high risk' types are associated with cervical dysplasias that have a considerable potential for progression to cancer 3 . An important emerging factor in the development of cervical neoplasia is the role of HPV variants 4 . HPV variants differ in biological and chemical properties and in pathogenicity and oncogenicity.
Prevalence of human papillomavirus genotypes in women from a rural region of Puebla, Mexico
International Journal of Infectious Diseases, 2009
Background-HIV-infected women have a high prevalence of human papillomavirus (HPV) infection and are more likely to be infected with HPV genotypes that are considered high-risk and have the potential for progressing to cervical cancer. The currently available HPV vaccines protect against specific HPV genotypes that may not be the most important causes of dysplasia and potentially of cervical cancer in HIV-1-infected women. African women have been underrepresented in the studies of global prevalence of HPV genotypes.
2013
Background: Infection with human papillomavirus (HPV) is associated with uterine cervical intraepithelial neoplasia (CIN) and invasive cancers (ICC). Approximately 80 % of ICC cases are diagnosed in under-developed countries. Vaccine development relies on knowledge of HPV genotypes characteristic of LSIL, HSIL and cancer; however, these genotypes remain poorly characterized in many African countries. To contribute to the characterization of HPV genotypes in Northeastern Tanzania, we recruited 215 women from the Reproductive Health Clinic at Kilimanjaro Christian Medical Centre. Cervical scrapes and biopsies were obtained for cytology and HPV DNA detection. Results: 79 out of 215 (36.7%) enrolled participants tested positive for HPV DNA, with a large proportion being multiple infections (74%). The prevalence of HPV infection increased with lesion grade (14 % in controls, 67 % in CIN1 cases and 88 % in CIN2-3). Among ICC cases, 89 % had detectable HPV. Overall, 31 HPV genotypes were d...
Assesment Of Hpv Dna Positive İndividuals İn Sivas Province İn Terms Of Some Variables
Cumhuriyet Medical Journal
Background: Cervical cancer is one of the most common cancers in women and causes death. Revealing the distribution of HPV prevalence and types in cervical cancer remains important for both treatment and vaccination strategies. The goal of this study is to determine the HPV DNA subtypes of women who are positive for Human Papilloma Virus (HPV) screening, and to evaluate their sociodemographic characteristics and cervical smear results. Methods: The study was conducted on 215 women who were directed to Sivas Cumhuriyet University Obstetry and Gynocology outpatient clinic by her gynocologists or family physicians between 2015-2018 years. A questionnary containing datas of patients about age, education levels and jobs of both spouses, smoking status, obstetric history and age of first sexual intercourse was filled out by the researcher. The data were analysed with SPSS (vers 23.0) program and significance level was taken as 0.05. Results: The most prevalent HPV types were type 16 and c...
Asian Pacific Journal of Cancer Biology, 2017
Background and objective: Considering the importance of HPV in cancer etiology, awareness of HPV prevalence and frequency of high-risk genotypes could help improve health care system management. We analyzed HPV prevalence in women forms different provinces of Iran that is the largest sample till now. Material and Methods: In this retrospective cross-sectional study, 2969 outpatient and suspicious women aged 17- 78 referred to Noor pathobiology laboratory from 24 provinces of Iran were studies. DNA extraction and PCR were performed on samples and then HPV genotypes were also determined using hybridization kit. Results: HPV DNA was detected in 29.3% of valid liquid-based samples. Among HPV positive cases, 67.2% and 52.0% accounted for high-risk and low-risk HPV subtypes, respectively. In patients with high-risk HPV types, HPV16 was confirmed as predominate type (30.5%) followed by HPV53 (17.3%) and HPV39 (13.3 %). HPV6 was found as the most common low-risk HPV type with 60.6% frequenc...
Research Square (Research Square), 2022
Purpose: Human papillomavirus is suggested as a well-known risk factor for cervical cancers and nowadays, primary HPV typing is recommended as an alternative test to cervical cytology. Methods: This cross-sectional study evaluated the liquid-based cervical smears of 700 women with no history of HPV vaccination and cervical dysplastic disease from 2017 to 2020 in Isfahan, Iran. Here, we try to compare the prevalence of HPV genotypes using COBAS (@roch) with the results of pap smear cytology in evaluating the most appropriate test for cervical cancer screening, especially in low-resource societies. Results: The prevalence of HPV infection was 23.3%, including 8.7% with HPV 16/18 and 14.6% with other HR (high-risk) HPVs. Considering positive HPV genotypes, the frequency of HR HPV decreases in older age groups; 42.1% compared to 6.8%. In cytology reports, 8 out of 16 individuals with high-risk lesions were negative for any type of HPV; on the other hand, there were 129 HR HPV-positive patients out of 570 negative or low-risk pap smear results. Conclusion: It assumed that there is no superiority for HPV genotyping over cytology or vice versa in detecting high-risk patients for cervical cancer; As only 26.8% of women with HPV show abnormal cytology; and from those with normal cytology, 17.9% were positive for HR HPV. Screening with one method had a rare but high risk of delay in the timely detection of cervical cancer. Above that, the high prevalence of HPV infection in unvaccinated women also reveals the need for studies on the costeffectiveness of universal vaccination.