Honey, polen, and propolis extracts show potent inhibitory activity against the zinc metalloenzyme carbonic anhydrase (original) (raw)
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Journal of enzyme inhibition and medicinal chemistry, 2016
Polyphenols are important secondary products of plants with the potential to inhibit carbonic anhydrases. The aim of this study was to investigate the inhibition effects of various phenolic standards, honey, propolis, and pollen species on human carbonic anhydrase I and II. The inhibition values (IC50) of the phenolics (gallic acid, protocatechuic acid, quercetin, catechin, tannic acid, and chrysin) ranged from 0.009 to 0.32 μg/mL, tannic acid emerging as the best inhibitor. The inhibition values of three different types of honey, heather, rhododendron, and chestnut ranged between 2.32 and 25.10 μg/mL, the chestnut honeys exhibiting the best inhibition. The ethanolic extracts of pollen and propolis exhibited good inhibitory properties, with IC50 values between 0.486 and 3.320 μg/mL. In order to evaluate the phenolic composition of bee products, phenolic profiles and total phenolic contents (TFC) were also measured. The inhibition ranking among the natural products studied was phenol...
Journal of Enzyme Inhibition and Medicinal Chemistry, 2012
Different natural products and secondary metabolites from mushrooms, teas, honeys, mosses, plants and seaweeds were investigated for their in vitro inhibitory effects on human carbonic anhydrase (hCA, E.C.4.2.1.1) isoforms I and II. Inhibition data were correlated with the total phenol content in the extract and investigated with the pure compounds believed to be responsible for this activity. Methanolic extracts were prepared for 17 such pure chemicals present in the natural products and for 41 diverse natural products. The IC 50 values were in the range of 0.11-66.50 μg/mL against hCA I and of 0.09-54.54 μg/mL against hCA II, respectively. The total phenol content was in the range of 0.02-1318.96 (as milligrams of gallic acid equivalents) per gram of sample. These data offer new insights on possible novel classes of CA inhibitors based on natural products, possessing a range of chemical structures not present in the classical inhibitors with pharmacological applications, such as the sulfonamides and sulfamates.
Determination of Enzyme Inhibition and Antioxidant Activity in Some Chestnut Honeys
Foods and Raw materials
Honey has always been seen as the main source of healthy natural food and folk medicines. It has been prized due to bioactive components that are responsible for different therapeutic effects. Phenolic compounds are one the parts of these components. It is claimed that these have been antioxidant agents. But it also has to be evaluated by different perspectives in biomechanics except antioxidative effects. A variety of diseases may be treated by the inhibition of some individual enzymes. A pharmaceutical drug and synthetic agents are used to treat and avert illness even though there is a potential risk named drug resistance. Nowadays, the most effective treatment seems to be the combined administration of natural foods. The study aims at investigating hyaluronidase (HYA), xanthine oxidase (XOD) and the urease enzyme inhibition of some chestnut honeys from different locations of Giresun and Ordu in Turkey. Moreover, the antioxidant activities of the prepared chestnut honey extracts were investigated by using different methods. The total phenolic (TP), total flavonoid (TF), FRAP, CUPRAC assays and DPPH, and ABTS inhibition potential were carried out using in vitro models. The enzyme IC 50 values in the samples ranged from 0.793 to 12.639 mg/ml for HYA; from 0.029 to 0.106 g/ml for XO; from 0.002 to 0.054 g/ml for urease, respectively. In conclusion, honey extracts exhibited good potentials towards the inhibition of activities of the studied enzymes, and the samples also suggest a practical value for surveying natural inhibitors for specific clinical purposes. Moreover, all results can provide a basis of future studies on the alternative medicinal application related to honey.
Indonesian Food and Nutrition Progress
Lead confers deleterious damage to the cells and it is necessary to explore further and develop a more effective way to ameliorate lead toxicity. This study aims to investigate how honey and Moringa oleifera (MO) can synergistically provide a more effective way to ameliorate lead toxicity. Groups of ten fish (Clarias gariepinus) were given Pb (0.30 g) and supplemented feed (T1) containing both honey (5 g) and MO (5 g). Others received pb (0.30 g) and supplemented feed (T2) containing either honey (10 g) or (T3) containing MO (10 g). Finally, hematological and biochemical analysis were conducted and a decline in the hematological parameters was observed. Also, the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea, creatinine, and malondialdehyde (MDA) significantly increased while glutathione (GSH) and superoxide dismutase (SOD) reduced in the group given lead only. However, these biochemical indices and hematological paramete...
Minerals, Toxic Heavy Metals, and Antioxidant Properties of Honeys from Bangladesh
The study reports on major and trace elements as well as antioxidant properties of honey samples from Bangladesh. Four major cationic elements, seven trace elements, and three heavy metals were determined in the 12 honey samples using atomic absorption spectrophotometer. Nutritional values in these honey samples were further investigated according to their antioxidant properties. The content of major elements was in the range of 62.75-616.58, 579.48-2219.43, 69.42-632.25, and 0.13-1.20 mg/kg for sodium, potassium, magnesium, and calcium, respectively. The trace elements varied in the range of 0.41-28, 0.12-3.54, 1.54-2.85, 0.29-0.59, 0.02-0.35, and 0.01-0.06 mg/kg for iron, zinc, copper, nickel, cobalt, and cadmium, respectively. Among the heavy metals, only lead (0.17-2.19 mg/kg) was detected. The results of antioxidant analysis based on phenolics, flavonoids, ascorbic acid, reducing sugar, and proteins (as nonphenolic antioxidants) revealed that multifloral raw honey samples contain significantly higher levels of reducing agents than monofloral and commercial brand honeys. The study provides a useful insight on the minerals, heavy metals, and antioxidant properties of honey samples commonly consumed in Bangladesh and found to be rich source of antioxidants and minerals. Some samples might pose some risk to the health due to lead contamination.
Evaluation of Lead, Cadmium and Copper Concentrations in Bee Honey and Edible Molasses
American Journal of Applied Sciences, 2010
Problem statement: Content of Cadmium, lead and copper in 26 bee honey samples from different places of Assiut governorate (south of Egypt) and three different botanical origins (Clover, Multi-flower and Citrus) in addition to four edible molasses samples from Egypt market were evaluated by Differential Pulse Anodic Stripping Voltammetry (DPASV) in Briton-Robinson buffer solution at pH ∼ 2.1, as well as atomic absorption spectrometry after wet digestion. Approach: The optimal deposition potentials and times for the detection of these metal ions in all sample solutions have been studied. Results: The concentration of each metal ion was determined by the standard addition method. The statistical parameters i.e., slope, standard deviation, correlation coefficient and confidence have been calculated. Conclusion/Recommendations: The results obtained using stripping voltammetry indicate that the average concentration of Cu ions ranged from 0.085-0.987 μg g −1. In addition, the average concentrations of Cd and Pb ions ranged 0.001-0.077 and 0.006-1.640 μg g −1 ; respectively. On the other hand, the average concentrations obtained using atomic absorption spectrometry of the same element mentioned above ranged from 0.077-0.991 μg g −1 for Cu; 0.001-0.087 μg g −1 for Cd and 0.007-1.650 μg g −1 for Pb.
Physicochemical and Residue Analysis of Honey from Black Sea Region of Turkey
2014
Honey samples obtained from Black Sea Region of Turkey were screened for physicochemical quality as well as the presence of heavy metals and minerals, veterinary drugs (antibiotics), pesticide and naphthalene residues. Mean values obtained for physicochemical parameters were 5.42±0.02 pH; 16.66±0.12% moisture; 67.54±0.49% invert sugar; 3.62±0.13% sucrose; 0.2±0.01% ash; 0.48±0.03 mS/cm electrical conductivity; 24.97±0.27 meq/kg acidity; 8.86±0.38 mg/kg hydroxymethylfurfuraldehyde, and 10.45±0.26 diastase activity. In the analysed samples, potassium was the major element, and manganese the minor one. Mean values obtained were (mg/kg): Ca, 4.83±0.33; K, 195.29±5.28; Mg, 19.97±0.88; Na, 10.75±0.23; Mn, 0.34±0.05; B, 0.71±0.04. The contents of heavy metals (Cu, 0.18±0.01; Zn, 0.16±0.01; Cd, 0.07±0.00; Fe, 1.66±0.04; Ba, 1.02±0.03; Ni, 0.14±0.01; Al, 0.021±0.001 mg/kg; Cr, 6.58±0.28; Pb, 6.99±0.31 µg/kg) were within permitted levels. 32.5 % of the examined samples had drug residues at le...
The Environmentalist, 2007
The possibility of finding new medicines from natural sources is one of the more commonly cited reasons for preserving biodiversity, and employing indigenous knowledge of traditional healing remedies greatly increases the likelihood of discovering these hidden medicinal compounds. The main difficulties in using natural products as a source for pharmaceutical lead compounds involve separating the plethora of compounds from the original extract, as well as the gamble of time and money invested in an activity that may not yield a novel compound. However, while these difficulties exist, the potential of natural products still far outweighs the limitations of the simple structures and known modes of action of synthetic lead compounds. As such, the production of novel medicines, particularly for cancer and human immunodeficiency virus (HIV) treatments as well as the inhibition of antibiotic-resistant bacteria, now requires the utilization of natural products. This paper provides a review of the current methods used in elucidating pharmaceutical lead compounds from natural sources, focusing on plant samples in particular.
Chemistry, Biology and Potential Applications of Honeybee Plant- Derived Products
2016
This chapter is focused on the application of electrochemical techniques (e.g., sensors and biosensors), as the predominant methodology, to the quantification of individual or total phenolic compounds, either in standard solutions or in real matrices (e.g., plants, fruits and beverages) and their capability for assessing antioxidant activity/capacity. Specially, the potential application to evaluate antioxidant capacity of beehives products (e.g., propolis, honey) is addressed. Finally, the voltammetric behavior of Portuguese monofloral honeys is discussed for the first time, taking into account the expected effects of honey color and floral origin.
Evidence-Based Complementary and Alternative Medicine, 2019
The aim of this in vitro study is to characterize the phenolic compounds of twelve honey samples collected from different locations in Palestine (H1-6) and Morocco (H7-12) and to evaluate their cytotoxic and cytostatic effects in cells from the human colorectal carcinoma cell line HCT-116 and breast cancer cell line MCF-7. Quantitative HPLC analysis revealed nine phenolic compounds in three Moroccan honey samples, namely, syringic acid, tannic acid, caffeic acid, ferulic acid, coumaric acid, gallic acid, rosmarinic acid, epicatechin, and pyrogallol. Syringic acid, abundant in numerous types of honey with strong antioxidant capacities, was present at values ranging between 0.10 mg/100 g and 1.24 mg/100 g of Daghmos (H11) and Kabbar (H10) samples, respectively. No significant reductions in cell viability were observed in both cell lines treated with the Palestinian samples as measured with MTT assay. Significant cytostatic effects were after treatment of HCT cells with Morar honey H1 ...