Evaluation of rice genotypes for brown planthopper (BPH) resistance using molecular markers and phenotypic methods (original) (raw)
Related papers
2015
Brown planthopper is one of the most destructive insect pest of rice in Indonesia and other Asian countries. Pyramiding some brown planthopper resistance genes is a valuable approach to create more durable resistance against the pest. The objective of this study was to identify polymorphisms of Brown Planthopper Resistance genes (Bph) on 20 genotypes of rice, and to obtain genetic relationship among genotypes tested. The experiment was conducted from June to September 2012 at Green House and Laboratory of Plant Analysis and Biotechnology, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor. Twenty genotypes were analyzed, and two of them were used as check varieties. Simple Sequence Repeat (SSR) markers were applied to detect Bph3, Bph4, Qbph3, and Qbph4 genes. Polymorphic levels were analyzed by calculating PIC (Polymorphic Information Content). The grouping of rice genotypes were done based on principal components analysis (PCA) of SSR data, and the genetic relationship ba...
Agronomy
Brown planthopper (BPH) is the most problematic insect in rice cultivation, as it decreases crop yields. In this study, 143 upland rice varieties were genotyped for five essential Bph resistance genes: bph2, Bph3, Bph14, Bph15, and Bph17. The gene frequencies of the five Bph resistance genes varied from 33.57% to 61.54%. The 139 varieties contained one to five Bph resistance genes. Polymorphism information content values ranged from 0.4460 to 0.4984 with an average of 0.4744. Cluster analysis supported the subpopulations identified by STRUCTURE. An analysis of molecular variance analysis identified 7% variance among and 92% variance within subpopulations, indicating a significant gene exchange between the two subpopulations. The evaluation of BPH resistance using the SEM system by IRRI showed that 2 varieties were resistant to BPH, 29 varieties were moderately resistant, and 112 varieties were susceptible. The Bph3, Bph14, and Bph15 genes and BPH resistance showed significant correl...
ScienceAsia
Three rice mapping populations of 208 BC1F2, 333 BC3F2 and 335 F2 lines derived from crosses of PTB33 × RD6, Rathu Heenati × KDML105 and IR71033-121-15 × KDML105, respectively, were used to detect brown planthopper (BPH) resistance genes. The modified mass tiller screening (MMTS) method was applied to evaluate the BPH resistance of all mapping population lines at the tillering stage. The BPH resistance genes detected from the BC1F2, BC3F2 and F2 populations were mapped in the same genomic region on the short arm of chromosome 6. The tightly linked markers RM589 and RM586 could explain 59.8%, 28.2% and 57.4 % of the phenotypic variance of the BPH resistance from the BC1F2, F2 and BC3F2, respectively. The tightly linked SSR markers identified from this study should be useful in marker-assisted breeding to produce BPH resistant cultivars.
The distribution and identification of brown planthopper resistance genes in rice
2009
A collection of 515 rice landraces originating from Vietnam and China were screened for the reaction to brown planthopper (BPH) infestation. Most of the resistant landraces were indica types from Vietnam and the Guangxi province in China. An F 2 mapping population was created from the cross between a BPH resistant Vietnamese landrace Yagyaw and the susceptible cultivar Cpslo17. Four quantitative trait loci (QTL) contributing to BPH resistance were mapped on chromosomes 2, 4, 7 and 9, respectively. The individual QTL accounted 5.64% to 12.77% of the phenotypic variance, and three resistant alleles were harbored in the resistant landrace Yagyaw. Two QTL located on chromosomes 2 and 4 were identified with significant additive effects and are useful in breeding new rice inbred lines. One resistant allele was harbored by the susceptible parent Cpslo17. This gene is important in selecting rice inbred lines with stronger resistances to BPH.
Hereditas, 2007
The brown planthopper (BPH) is one of the most serious insect pests of rice throughout Asia. In this study, we constructed a linkage map to determine the locus for BPH resistance gene, using an F2 population from a cross between a resistant indica cultivar, ‘Col.5 Thailand’, and a susceptible cultivar ‘02428’. Insect resistance was evaluated using 147 F3 families and the genotype of each F2 plant was inferred from the phenotype of corresponding F3 families. Two QTLs was detected on chromosome 2 (explains 29.4% phenotypic variation) and 6 (46.2% variation explained) associated with resistance to BPH in the mapping population. Comparison of the chromosomal locations and reactions to BPH biotypes indicated that the gene on chromosome 6 is different from at least 18 of the 19 previously identified BPH resistance genes. These two genes have large effects on BPH resistance and may be a useful BPH resistance resource for rice breeding programs.
Breeding Science, 2010
The brown planthopper (BPH) resistance gene bph4 has previously been assigned on the short arm of rice chromosome 6. However, the map position of the gene could not be determined. To detect the bph4 locus, 15 polymorphic simple sequence repeat (SSR) markers covering genetic distance of 0.0-63.4 cM on chromosome 6 were used to survey 15 BPH resistant (R) and susceptible (S) individuals from each of the 95 and 78 F 2 populations derived from crosses of TN1/Babawee and Babawee/KDML105, respectively. One SSR marker, RM586, was associated with the R and S from the F 2 populations. Additional markers surrounding the RM586 locus were examined to define the location of bph4. From the genetic linkage map and QTL analysis of 95 and 78 F 2 individuals, the bph4 locus was mapped at the same chromosomal region of Bph3 between two flanking markers RM589 and RM586. Markers linked to the resistance gene explained 58.8-70.1% of the phenotypic variations and can be used for marker-assisted selection in BPH-resistant breeding programs. In addition, our experiment provides evidence that a recessive gene could behave as a dominant gene under different genetic backgrounds.
Indonesian Journal of Agricultural Science
Resistance traits to brown planthopper on rice varieties are controlled by dominant and recessive genes called Bph/bph. Bph17 is one of dominant genes that control rice resistance to brown planthopper. Marker of Bph17 allele can be used as a tool of marker assisted selection (MAS) in breeding activity. Association of Bph17 allele and resistance to brown planthopper in Indonesian landraces and new-improved varieties of rice is not clearly known. The study aimed to determine the association of Bph17 allele in landraces and new-improved varieties of rice resistant to brown planthopper. Twenty-one rice genotypes were used in the study, consisting of 13 landraces, 5 improved varieties, 3 popular varieties and a check variety Rathu Heenati. Two simple sequence repeat markers linked to Bph17 allele were used, i.e. RM8213 and RM5953. The results showed that association of Bph17 allele in landraces and new-improved varieties of rice resistant to brown planthopper resistance was very low (r =-0.019 and-0.023, respectively). The presence of Bph17 allele did not constantly express resistance to brown planthopper. The study suggests that Bph17 allele cannot be used as a tool of MAS for evaluating resistance of landraces and new-improved varieties of rice to brown planthopper. Further research is needed to obtain a specific gene marker that can be used as a tool of MAS and applicable for Indonesian differential rice varieties.
Journal of Pharmacognosy and Phytochemistry, 2016
The brown planthopper (BPH), Nilaparvata lugens Stal (Homoptera: Delphacidae), is the most-significant insect pest of rice (Oryza sativa L.) throughout rice-growing countries. In this study DNA-based SSR molecular marker technique was deployed to estimate genetic diversity among 30 rice accessions using seven SSR primers which produced a total of 32 polymorphic markers with the average number of 4.57 markers per primer. The PIC value for all 7 microsatellite loci in the present study ranged from 0.500-0.833. The PIC value was maximum in case of RM186 (0.833) and all the SSR markers were more informative since they recorded more than 0.500 as PIC value. The seven primer pairs showed 3-6 alleles across 30 genotypes grouping the genotypes into 7 groups of different composition indicating that even seven SSR primer pairs can differentiate the cultivars. The analysis indicated that co efficient of similarity among 30 varieties ranged from 0.67 to 1. In the present study, SSR markers were...
Evaluation of rice genotypes for brown planthopper resistance
Journal of Agriculture and Value Addition, 2020
Brown planthopper (Nilaparvata lugens) (BPH) is a major pest of rice in Sri Lanka. Identification of new resistance sources has immense important in varietal improvement programme. Therefore, twenty-five rice genotypes including new improved, exotic and traditional varieties were evaluated for BPH resistance using standard seed box screening technique and honeydew test to identify resistant varieties. During screening Ptb 33 was used as resistant variety. TN1 and Bg 380 were used as susceptible varieties. Bg 380 showed highly susceptible reaction. Ptb 33 had the highest level of BPH resistance with lowest damage score of 3.0. Bg 300, Bg 352, Bg 379-2, Bg 450 and Bw 367 which are popular varieties recorded damage score of 4.7 to 5.6 which categorized as moderately resistant reactions. Similarly, two exotic lines, IR 65482-7-216-2 and IR 71033-121-15 showed moderately resistant reaction too. Among the traditional rice cultivars, Mudukiri el, Horana ma wee, Hondarawala and Mada el showed resistant to moderately resistant reactions. According to the present study, Bg 379-2 and IR 71033-121-15 were the better donor parents for BPH resistance since those varieties having good plant architecture and yield.
Euphytica, 2017
Crown rot (CR), caused by various Fusarium species, is a major disease in many cereal-growing regions worldwide. Fusarium culmorum is one of the most important species, which can cause significant yield losses in wheat. A set of 126 advanced International Maize and Wheat Improvement Center (CIMMYT) spring bread wheat lines were phenotyped against CR for field crown, greenhouse crown and stem, and growth room crown resistance scores. Of these, 107 lines were genotyped using Diversity Array Technology (DArT) markers to identify quantitative trait loci linked to CR resistance by genome-wide association study. Results of the population structure analysis grouped the accessions into three subgroups. Genome wide linkage disequilibrium was large and declined on average within 20 cM (centi-Morgan) in the panel. General linear model (GLM), mixed linear model (MLM), and naïve models were tested for each CR score and the best model was selected based on quarantine-quarantine plots. Three marker-trait associations (MTAs) were identified linked to CR resistance; two of these on chromosome 3B were associated with field crown scores, each explaining 11.4% of the phenotypic variation and the third MTA on chromosome 2D was associated with greenhouse stem score and explained 11.6% of the phenotypic variation. Together, these newly identified loci provide opportunity for wheat breeders to exploit in enhancing CR resistance via marker-assisted selection or deployment in genomic selection in wheat breeding programs.