Evaluation of Vaccination Efficacy of Live recombinant Leishmania tarentolae Expressing KMP-11–NTGP96-GFP fusion in Combined with Aluminum Hydroxide Gel adjuvant against cutaneous Leishmaniasis (original) (raw)

Abstract

Backgrounds: : Adjuvants are immunological agents that added to vaccine to modify the immune response .It has been known that KMP-11 is a fully protective antigen and NT-Gp96 acts as a strong biologic immunologic adjuvant. We used non-pathogenic Leishmania tarentolae to deliver these specific genes and combination of it with Alum was evaluated against cutaneous Leishmaniasis. Materials and Methods: Efficacy of recombinant Leishmania tarentolae and combination of it with Alum against cutaneous Leishmaniasis was tested in susceptible BALB/c mice. Both humoral and cellular immune responses were assessed before and at 4 weeks after challenge with Leishmania major infection. The results: The strongest protective immunity were observed with live recombinant L. tarentolae alone but it seems that Alum combination could not protect against infection. Conclusions: This study is the first to use a combination of live non pathogenic recombinant L. tarentolae parasite with Alum adjuvants and see...

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