Agreement of two different laboratory methods used to measure electrolytes (original) (raw)
Related papers
Comparison of electrolyte levels in serum and plasma
IP Innovative Publication Pvt. Ltd, 2017
Introduction: Measurement of electrolytes plays a significant role in the management of critically ill patients. The accuracy of electrolyte measurement depends on a number of pre-analytical variables. Electrolytes are the positively and negatively charged ions that are essential for normal cellular functioning of the body. Most of the metabolic processes are mediated through electrolytes. Electrolyte abnormalities are seen in a number of conditions and can end up in life threatening conditions unless accurate results are given from the laboratory. Aim: To check the effect of different storage temperature on estimation of electrolyte values using serum and plasma samples of venous blood. Materials and Method: Analysis of electrolyte levels was conducted in venous blood samples collected from 30 patients stored under room temperature and at 2-4 0 C. Serum and plasma electrolytes-sodium [Na], potassium [K], chloride [Cl] were analysed using electrolyte analyser at different time intervals and storage conditions. The samples at room temperature were analysed immediately after collection and at 6hours, 12hrs, 24 hrs, 36hrs and 48hrs.The samples kept at 2-4 0 C were also analysed at 6hrs, 12hrs, 24 hrs, 36hrs and48hrs simultaneously. Results: Serum K, Cl has no significant changes till 48 and 12hrs, both at 2-4 0 C and at room temperature respectively. Serum Na at 2-4 0 C is relatively stable for a longer time than its counterpart stored at room temperature (48hrs vs 36 hrs). Similarly, plasma samples also showed more stability at 2-4 0 C than at room temperature. Conclusion: Estimation of electrolytes as early as possible at proper storage conditions is the best solution to avoid analytical errors.
Study of Serum Electrolytes by Flame Photometer and Autoanalyser
Electrolytes are present in the human body, and the balance of the electrolytes in our body is essential for normal functioning of our body. Common electrolytes important for quantitative estimation includes sodium and potassium. In 1930's, chemical methods were used to measure serum electrolytes. To overcome disadvantages like time consuming process, little accuracy, flame photometer was devised and after three decades, autoanalyser was introduced. Measurement of serum electrolytes by means of Sherwood Scientific model 420 flame photometer were compared with measurements made by Fully Automated Olympus AU400 autoanalyser to determine the efficiency of instrument. Determination of serum electrolytes showed slight difference in the results. Measurement of serum electrolytes by Fully Automated Olympus AU400 autoanalyser was faster and with less quantity of serum sample as compared to Sherwood Scientific Model 420 flame photometer which requires diluted serum sample, which may introduce manual error. Fully Automated Olympus AU400 autoanalyser measures serum electrolytes with ion-selective electrodes (ISE) which depends on the ionic activity, whereas flame photometer measures the stoichiometric concentration. Determination of serum electrolytes by Olympus AU400 autoanalyser was more efficient, sensitive and accurate than Sherwood Scientific Model 420 flame photometer.
Journal of Medical Diagnostic Methods, 2017
Our aim is to determine whether the serum electrolytes (Sodium and Potassium) measured by two different methods on two different equipments are equivalent or not. This retrospective study was conducted over three months period (June 2017-August 2017). A total number of 300 patients from intensive care unit or in the different wards with a variety of diagnoses were enrolled in this study. Analysis of sodium and potassium levels in their serum was carried out using an electrolyte analyzer and an auto-analyzer. Statistical measures were applied using students paired t-tests. The Mean level (± standard deviation) of sodium measured by electrolyte analyzer was statistically significantly higher than the auto-analyzer values (139.99 ± 7.48 mmol/l and 137.15 ± 7.66 mmol/l respectively; P˂0.0001). Regarding the potassium levels, the Mean level (± standard deviation) measured by electrolyte analyzer was statistically significantly greater than that of potassium measured by auto-analyzer (4.290 ± 0.743 mmol/l and 4.147 ± 0.738 mmol/l respectively; p˂0.0001). Our results showed that serum electrolytes levels measured by electrolyte analyzer were higher than those measured by auto-analyzer. Differences obtained were statistically significant.
Reliability of blood gas analyzer for the measurement of electrolytes -A comparative study
2016
Background: Electrolyte and blood gas analysis are essential for most patients who are admitted in the casualty. Routinely simultaneous arterial and venous sample would be sent to the laboratory for blood gas analysis and serum electrolytes respectively. Electrolytes can also be measured by the blood gas analyzer. We wanted to find out if these values were reliable. Methods: This study has been checked and permitted by the hospital ethics committee. We obtained two samples, one venous and one arterial sample from patients in the intensive care unit (ICU) and casualty of Ashwini rural medical college, hospital and research centre, (ARMCH&RC) Kumbhari Solapur. The arterial blood samples were analyzed on the ABOTT arterial blood gas analyzer (ABG) and venous samples were analyzed on PROLYTE electrolyte autoanalyzer (AA). Both instruments were placed in our biochemistry laboratory. Results: A total of 200 samples were analyzed. The mean ABG sodium value was 132.0 mmol/L (SD 9.82) and the mean AA sodium (Na +) was 135.7 mmol/L (SD 9.99). The mean AA sodium value was more than mean ABG sodium but statistically significant difference was not found (p=0.18). The mean ABG potassium (K +) value was 3.4 mmol/L (SD 0.68) and the mean AA potassium (K +) was 3.8 mmol/L (SD 0.79). The mean AA potassium value was more than mean ABG potassium and found statistically significant difference (p=0.02). Conclusion: We found that there was no significant difference in the sodium values measured by the ABG analyzer and the auto-analyzer. However, there was a significant difference in the potassium values measured by these instruments.
Flame photometry is appropriate for qualitative and quantitative estimation of few cations, particularly for metals, Ion-selective electrodes (ISEs) which react generally particularly to different particles (both anions and cations) work on the same principle, The purposes of present study is to estimation of serum electrolytes by method of BWB-XP Flame Photometer were compared and estimations made by HACH HQ440d multi-ion selective electrodes to decide the proficiency of instrument.
world Journal of Advanced Research and Review, 2022
Background: the blood electrolytes analysis is a routine laboratory test which proper execution would help in the diagnosis of hydro-electrolytes disorders. The objective of this work was to assess the internal quality control of the sodium and potassium tests from the pre-pre-analytical phase to the post-analytical phase. Material and Methods: This was a cross-sectional study which took in the laboratory of biochemistry at the Institute of Cardiology, Abidjan, Ivory Coast from March 1 st to March 31, 2009. We used the flame photometer to measure the sodium and potassium electrolytes level in the internal control Exatrol-Normal from Biolabo ®. Clinical samples were also taken for the determination of the same electrolytes levels. The pre-pre-analytical quality indicators depending on the physicians order, the pre-analytical quality, the analytical quality and the post-analytical indicators under the control of the laboratory were assessed by using "NF en ISO 15189 version 2007" check list: Particular requirements for quality and competence for biomedical laboratories paragraph 5.4.1, 5.4.2, 5.5 and 5.7. Data were captured into Microsoft Excel [Microsoft Corporation, Redmond, WA] and then imported and analyzed using QI Macros SPC Software for Excel ®. The levels of Na + and K + in the control material Exatrol Normal from Biolabo ® were represented as follow: mean (m), Standard deviation (SD). The values of the monthly distribution of Na + and K + concentrations around the mean were used to draw the Levey-Jennings diagram and Wesgard's rules were used to evaluate the performances of the analytical process. Results: a total of 112 electrolytes analysis order were received at the biochemistry laboratory. For the pre-preanalytical phase, the analysis of these requests forms revealed that 81 (72.3%) requests forms carried no clinical information. The non-compliance of the samples were mainly represented by the sampling under tight tourniquet 4 (3.6%), followed by the non-respect of the succession of tubes during multiple sampling process 3 (2.7%). For the analytical phase, the monthly Levey-Jennings diagram showed a dispersion of the two electrolytes Exatrol-Normal Biolabo ® levels between the mean plus or minus 2 standard deviations [m ± 2SD]: 139.34 ± 2.84 mmol/L for Na + and for K + , between [m± SD]: 4.2 ± 0.78 mmol/L. The analytical performances assessment for the two Levey-Jennings diagrams by using Wesgard's rules did not found any significant critical deviations with regard to the distribution of Na + and K + levels. For clinical samples, isolated hyponatremia was the most common disturbance (30.4%) followed by isolated hypokalemia (12.5%). At the post-analytical phase we observed for test execution a mean turnaround time of 34 ± 5.2 minutes with extremes ranging from 23 to 95 minutes. One case (0.9%) of transcription error was noted. Conclusion: the internal quality control process is applied in the clinical biochemistry laboratory at the Institute of Cardiology, Abidjan. A systematic verification system of the different phases of the analytical process helped to follow quality indicators at all levels of the pre, analytical and post analytical process and corrective actions were taken if
Objectives Accurate electrolyte results are important for management of critically ill patients hence this retrospective study was planned to compare these results processed on different instruments using the same direct ion-selective electrode technology. Results There was a total of 500 patients for comparative analysis 284 males and 216 females with a mean age of 54 years. The values for electrolytes were higher on electrolyte analyzer as compared to ABG analyzer for sodium they were 136.70±9.28 mEq/L and135.30±11.66 mEq/L, (p<0.01) and for potassium they were 4.26 ±1.10 mEq/L and 3.50±1.03 mEq/L respectively (p<0.05). the difference observed in the two instruments was statistically significant. Conclusions The results of the ABG analyzer can be used as a guideline to initiate primary treatment for critically ill patients but decide on definitive treatment only after the availability of serum electrolyte results.
Evaluation of an Automated Selective-Ion Electrolyte Analyzer for Measuring Na+, K+, and Cl-in Serum
Clinical Chemistry, 1974
analyzer, in which ion-selective electrodes are used to measure sodium, potassium, and chloride in serum, was assessed in a clinical sethng. Day-to-day precision, evaluated by replicate analysis of serum pools, yielded the following coefficients of variation for sodium, potassium, and chloride, respectively: 0.99%, 1.39%, and 0.67%. Values for chloride in both commercial control sera and aqueous standards were linearly related to concentration over a range of at least 10-220 mmol/liter; however, results with the potassium and sodium electrodes showed slight curvilinearity over the range 0-24 and 10-220 mmol/liter, respectively.
Journal of Global Pharma Technology, 2018
Introduction: Electrolytes measurement is a standard procedure in medical practice to diagnose diseases or disease-related complication. Recently, there is a various device used to analyze blood electrolytes, but the difference accuracy between devices is a problematic issue for clinicians. Thus, we evaluate the differences of sodium and potassium concentration reading by Blood Gas Analyzer (BGA) and Electrolyte Analyzer at Sanglah Hospital to assess whether the difference could be accepted according to Clinical Laboratories Improvement Amendments (CLIA). Methods: A cross-sectional analytic study was conducted in May 2017 at Clinical Pathology Laboratory of Sanglah Hospital, Denpasar. 30 subjects were enrolled consecutively during the study period and the blood samples were analyzed by both BGA and electrolyte analyzer. The data were analyzed using paired T-Test, Bland-Altman Plot, and Linear Regression. Result: The mean of sodium and potassium measured by BGA were 133.76 ± 5.68 mm...
Türk biyokimya dergisi, 2019
Aim: Our study was aimed to compare two different laboratory methods used to measure serum sodium and serum potassium level i.e. between the indirect Ion Selective Electrode (ISE) based and Enzymatic based method in Beckman Coulter AU-480, USA, in serum samples. Materials and methods: This was a cross sectional single center study carried out from September'16 to January'17 on 130 serum samples, to compare the result of serum sodium (S. Na +) and serum potassium (S. K +) level by indirect ISE and Enzymatic method in fully automated biochemistry analyzer (Beckman Coulter AU-480, USA). Results: To validate new enzymatic method bias, CV% and correlation coefficient were measured for S. Na + and S. K +. The study has drawn a considerable agreement for measurement of S. K + level between two methods with mean ± SD of difference was 0.14 ± 0.22 (p = 0.2242), CV% 2.7% and r 2 value of 0.94, whereas disagreement for measurement of S. Na + level with mean ± SD of difference was 2.38 ± 2.36 (p < 0.01), CV% 0.5% and r 2 value of 0.82. Bias, CV% and correlation coefficient are acceptable for S. K + , whereas nonacceptable for S. Na + level measurement as per Clinical Laboratory Improvement Amendments (CLIA) guidelines. Conclusion: There was a good correlation in S. K + between both methods whereas values were not correlated well with S. Na + level. So, enzymatic method can be used as an alternative to indirect ISE method for estimation of S. K + in small laboratories due to its cost effectiveness, but still intense workout is required for estimation of S. Na + .