Phytochemical and Biological Studies on Bark Extract of Aporosa wallichii Hook.f (original) (raw)
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In-vitro Biological Screening of Methanol Extract of Aporosa wallichii ( Euphorbiaceae ) Leaves
2017
This research was conducted to find out and evaluate potential biological properties of the medicinal plant called Aporosa wallichii which belongs to family Euphorbiaceae. To fulfill the research determinations several experiment were done like antioxidant property test which include DPPH test and total phenolic content test, brine shrimp lethality assay, thrombolytic property test and antimicrobial property test. After completing these experiments evident from various observation that, this plant showed good level of antioxidant property, moderate level of thrombolytic property and significant level of cytotoxicity property. Additionally, antimicrobial property test did not showed desired result for this plant, which indicate it may not contain any anti-microbial property. However, this research is initial to the finding of pharmacological property of this plant. Finally, on the basis of this research it can be claimed that this plant Aporosa wallichii able to provide good impact in medicine world and development of global healthcare.
Antioxidant Activities and Phenolic Content of Piper wallichii (Miq.) Hand.-Mazz
International Journal of Food Properties, 2013
The antioxidant activities of ethanol, methanol, chloroform, ethyl acetate, and petroleum ether extracts from the fruit of Piper wallichii (Miq.) Hand.-Mazz. were investigated using different in vitro antioxidant assays. The methanol extract showed the most potent scavenging activity on 2,2-diphenyl-1-picryl hydrazyl (DPPH), Ferric reducing antioxidant power (FRAP), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) hydrogen peroxide, hydroxy radical, and reducing power assays with EC 50 values of 46.70 ± 0.85, 41.7 ± 0.74, 45.23 ± 2.02, 49.30 ± 1.15, 40.39 ± 1.92, and 50.21 ± 1.2 µg/ml, respectively. The total phenolic content and the total flavonoids content were also determined. The methanol extract showed the highest total phenolic content and total flavonoids content, followed by the chloroform extract. This study revealed that the methanol extract had the strongest antioxidant activities, which were correlated with its high level of phenolic and flavonoids content.
Jurnal kimia terapan Indonesia, 2017
Active compounds with antioxidant activity were isolated from ethyl acetate extract of namnam stem (C. cauliflora L.) that had undergone maceration and fractionation by gravity column chromatography. The compounds were later identified by by using UV-Vis Spectrophotometry, FTIR, LCMS and 1 H-NMR. Ethyl acetate extract of namnam stem showed considerably high antioxidant activity (IC 50 value 4.68 ± 0.035 ppm). The results of analysis by UV-Vis and FTIR showed carbonyl group conjugated with an aromatic ring DW EDQG , max 330.22 nm), chromophore group of alkene (C=C) at band II max QP DQG IXQFWLRQDO JURXSV VXFK DV 2í+ FP-1), C=O (1729.23 cm-1), C=C (1652.64 and 1611.99 cm-1 &í2 DQG &í+ ortho (738.23 cm-1). LCMS (m/z 270.9246) and 1 H-NMR data showed seven proton signals on the aromatic ring at carbon position C-3 at / H 6.86 ppm (1H, s), C-6 at / H 5.95 ppm (1H, d, J=1.95 Hz), C-8 at / H 6.25 ppm (1H, d, J= 1.95 Hz), C-¶ and C-¶ at / H 7.03 ppm (2H, d, J=7.87 Hz), C-¶ and C-¶ at / H 6.87 ppm (2H, d, J= 7.87 Hz) so that the structure was identified as a flavonoid which was 4 ', 5,7-trihydroxy-flavones or known as apigenin. The isolated apigenin had very strong antioxidant activity, as shown by IC 50 value of 5.18 ± 0.014 ppm.
Plants
Apocynum hendersonii is a traditional medicinal plant used primarily as tea. It has a potential health benefit from its rich bioactive substances. This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf. The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC). The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides. Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW). It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-d...
BMC Chemistry
Background: As possible sources of natural bioactive molecules, the plant essential oils and extracts have been used globally in new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease. Methods: In this research, the antimicrobial activities of chloroformic and methanolic extracts of Sophora flavescens, Rhaponticum repens, Alhagi maurorum, Melia azedarach, Peganum harmala, and Juncus conglomeratus were evaluated against 8 bacteria (S. aureus, B. subtilis, R. toxicus, P. aeruginosa, E. coli, P. syringae, X. campestris, P. viridiflava) and 3 fungi (Pyricularia oryzae, Fusarium oxysporum and Botrytis cinerea), through disc diffusion method. Furthermore, the essential oils of plants with the highest antibacterial activity were analyzed utilizing GC/MS. Moreover, the tested plants were exposed to screening for possible antioxidant effect utilizing DPPH test, guaiacol peroxidas, and catalase enzymes. Besides, the amount of total phenol and flavonoid of these plants was measured. Results: Among the tested plants, methanolic and chloroformic extracts of P. harmala fruits showed the highest antibacterial activity against the tested bacteria. Besides, the investigation of free radical scavenging effects of the tested plants indicated the highest DPPH, protein, guaiacol peroxidase, and catalase in P. harmala, M. azedarach, J. conglomeratus fruits, and J. conglomeratus fruits, respectively. In addition, the phytochemical analysis demonstrated the greatest amounts of total phenolic and flavonoid compositions in J. conglomeratus and P. harmala, respectively. Conclusion: The results indicated that these plants could act as a promising antimicrobial agent, due to their short killing time.
International Journal of Pharmacy and Pharmaceutical Sciences, 2017
Objective: The current investigation involves the isolation, characterization and prediction of biological activity spectra of the phytoconstituents from the ethanolic extract of the heartwood of Pterocarpus marsupium roxb. (Fabaceae). Methods: The heartwood (3 kg) was extracted in alcohol by cold maceration for 21 d and the compounds were isolated by column chromatography. The compounds thus isolated were characterised and their structures were elucidated by using assorted spectral data analysis, i.e., infrared radiation spectroscopy (IR), proton nuclear magnetic resonance (1 HNMR), carbon thirteen nuclear magnetic resonance (13 Results: Phytochemical investigation of ethanolic extract of the heartwood of Pterocarpus marsupium led to isolate two fatty esters and a phenolic compound characterised as n-octanyl n-octadeca-9,12-dienoate (n-octanyl linoleiate, 1), n-dodecanyl n-octadeca-9,12-dienoate (n-dodecanyl linoleiate, 2) and 2, 3-dioxymethylene phenol (3). These phytoconstituents are reported for first time in the heartwood of Pterocarpus marsupium Roxb. The in silico profiling of these phytoconstituents exhibited their broad spectra of biological activity. Compounds (1) and (2) showed their maximum activity as All-trans-retinyl-palmitate hydrolase inhibitor, anti eczematic, lipid metabolism regulator, etc. and compound (3) was found to be active as membrane integrity agonist, aspulvinone dimethylallyl transferases inhibitor, carminative, neurotransmitter uptake inhibitor, etc. C NMR) and direct analysis in real time mass spectrometry (DART-MS). PASS (prediction of activity spectra for substances) computer program was used to predict the biological activity spectra of the isolated compounds. Conclusion: These isolated phytoconstituents can be used as the marker compounds to establish the identity, quality and purity of the drug. The results of PASS prediction shall be very useful for establishing these phytoconstituents as active pharmacological moieties.
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Context: Albizia odoratissima (L. f.) Benth has been used in Indian folk medicine to treat numerous inflammatory pathologies, such as leprosy, ulcers, burns and asthma. Objective: To evaluate the antioxidant and antimicrobial properties of A. odoratissima. Materials and methods: Dried leaves of A. odoratissima were extracted in organic solvents (hexane, chloroform, ethyl acetate, and methanol). The total phenolic content (TPC) and total flavonoid content (TFC) were determined using the Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. The antioxidant activity was examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (H 2 O 2), 2,2 0-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), and ferric reducing antioxidant power (FRAP) assays. The antibacterial activity was examined using minimum inhibitory concentration (MIC) and the minimum bacterial concentration (MBC), determined by broth microdilution method against Gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Proteus vulgaris) and Gram-positive bacterium (Staphylococcus aureus). Results: The TPC ranged from 4.40 ± 1.06 to 1166.66 ± 31.85 mg GAE/g of dry weight (DW), and the TFC ranged from 48.35 ± 3.62 to 109.74 ± 1.84 mg QE/g of DW. The IC 50 values of the ethyl acetate extract for DPPH, ABTS, and H 2 O 2 were 10.96 ± 0.40, 4.35 ± 0.07, and 163.82 ± 1.52 lg/mL, respectively. Both methanol and ethyl acetate extracts demonstrated effective antibacterial activity with MICs and MBCs values ranging 136-546 lg/mL and 273-1093 lg/mL, respectively, against the tested pathogenic species. Conclusions: The leaves of A. odoratissima showed potent free radical scavenging property and antimicrobial activity.
Radovi Šumarskog Fakulteta Univerziteta u Sarajevu, 2018
The aim of this study was to determine the total concentrations of some phenolic compounds and antioxidant and antimicrobial activity of methanol extracts of different parts of Illyrian endemic Petteria ramentacea. Concentrations of phenolic compounds were determined with UV/VIS spectrophotometry. The antioxidant activity of plant extracts was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Antimicrobial activity of extracts was evaluated by measuring the inhibition's zone against six selected test bacteria and two fungi. The highest average total phenols concentrations were in seeds (10.78 mg GAE g-1 DW), root (10.51 mg GAE g-1 DW) and bark (10.40 mg GAE g-1 DW), and the lowest in inflorescences (2.99 mg GAE g-1 DW) and leaves (3.12 mg GAE g g-1 DW). The total flavonoids concentrations were determined only in leaves (8.25 mg CE g-1 DW) and in stem (5.66 mg CE g-1 DW). Both flavanols and proanthocyanidins (0.75 mg CE g-1 DW and 3.49 mg CE g-1 DW, respectively) were found only in leaves. Analysis of variance indicated presence of significant differences between total phenols and flavanols concentrations (p<0.05), and Duncan's test confirmed the presence of intraspecies variability according to their concentrations. The highest antioxidant activity (IC 50) was observed for the seed's extract (6.86%) and the lowest for the bark's (51.31%). All methanol extracts showed the most pronounced antibacterial activity against Staphylococcus epidermididis, and the lowest against S. aureus subsp. aureus. Antifungal activity against Candida albicans was moderate. Since Peteria ramentacea methanol extracts are potential natural antioxidant and antimicrobial preparations against selected microorganisms, it is necessary to continue with more detailed analysis.
Bioactive compositions and identification of functional groups of selected medicinal plants
GSC biological and pharmaceutical sciences, 2024
Medicinal plants, either as standardized extracts or in their pure forms, offer countless propects for new drug leads and also possess a good range of nutritional benefits due to the presence of bioactive organic chemical compounds called phytochemicals that act as a defense against a variety of diseases. This study focus was to investigate the bioactive compounds, and identification of functional groups of three medicinal plants; Monodora myristica, Parkia biglobosa, and Azadirachta indica. The solvents used were methanol, water and ethanol respectively. Gas Chromatography-Mass Spectroscopy (GC-MS) assay was used for the analysis of bioactive compounds and Fourier-Transform Infrared Spectroscopy (FTIR) method was used for the identification of functional groups in the plant extracts. Results revealed several bioactive compounds at different peaks that were confirmed by their retention time, compound name, structure and percentage composition. FTIR analysis revealed different functional groups with distinct characteristic wave numbers, peak intensity, peak shape and bonds in the plant extracts. Among the identified phytocompounds of Monodora myristica, phenol, dodecanoic acid, hexadecane, n-hexadecanoic acid, 1-octadecene, and 1-eicosanol possess antioxidant property. Similarly, some of the identified phytocompounds of Parkia biglobosa; dodecanoic acid, hexadecanoic acid, 9-octadecene, naphthalene, 3-eicosene and n-hexadecanoic acid possess antioxidant property. Likewise, Hexadecane, hexadecenoic acid, 3-eicosene and n-hexadecanoic acid are phytocompounds of Azadirachta indica with antioxidant property. This study demonstrated these plants as a great source of naturally occurring bioactive compounds with therapeutic value, which supports their application in medicine to treat a variety of diseases.