A new method of detecting changes in corneal health in response to toxic insults (original) (raw)

This study introduces a new metric, edge-fibrillar distance (EFD), to evaluate the spatial arrangement of collagen fibrils in the cornea in response to two multipurpose solutions (MPS): ReNu and Hippia. It was found that corneas soaked in ReNu exhibited partially degenerated epithelium but uniformly distributed collagen fibrils, while those in Hippia showed loss of epithelium with closely packed collagen. Statistical analysis revealed significant changes in collagen fibril diameters and spacing compared to untreated corneas, suggesting the EFD and its normalized standard deviation (NSDEFD) can be useful parameters for assessing corneal health and transparency.