Advantages of “Headspace” technique for GC/MS analysis of essential oils (original) (raw)
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ADVANTAGES OF "HEADSPACE" TECHNIQUE FOR GC/MS ANALISYS OF ESSENTIAL OILS
The essential oils of Fir needle and Juniper obtained in Romania by industrial scale steam distillation of fresh material collected from wild flora were analyzed by gas chromatography-mass spectrometry (GC-MS), using two different techniques of injection: "split-splitless" and "headspace" (HS/GC-MS). The determined chemical composition of each essential oil was compared with literature data and previous results obtained by the authors. Qualitative and quantitative differences were obtained between the two injection used techniques.
2016
Essential oils are greatly strenuous aromatic materials having various constituents. They are used in the preparation of various precious substances like making perfumes, medicines, cleaning agent, and aromatic treatment etc. The purpose of the present investigation was to identify the major and minor chemical constituent in eighteen essential oils viz., amyris, basil, black pepper, camphor, catnip, chamomile, cinnamon, citronella, dill, frankincense, galbanum, jasmine, juniper, lavender, peppermint, rosemary, tagetes and thyme with the help of gas chromatography /mass spectrometry (GC/MS). In eighteen essential oils the identified compounds studied by GC-MS contain various types of high and low molecular weights of chemical ingredients. Therefore, GC/MS efficiently and speedily screened all the volatile elements present in the essential oils for the quantitative use of these identified chemical constituents for various reasons
Essential oils and volatiles: sample preparation and analysis. A review
Flavour and Fragrance Journal, 2010
This article is a short overview on the state of the art in essential oil analysis. Several aspects of the analysis of essential oils and volatile fraction of vegetable matrices are here critically discussed. The following topics are dealt with steam distillation and hydrodistillation and headspace sampling for sample preparation, and fast-GC and fast-GC-QMS analysis, enantioselective GC, multidimensional GC techniques, GC-Isotopic Ratio Mass Spectrometry (GC-IRMS) for analysis and quantitation.
A quantitative analysis of the individual compounds in tobacco essential oils is performed by comprehensive two-dimensional gas chromatography (GC × GC) combined with flame ionization detector (FID). A time-of-flight mass spectrometer (TOF/MS) was coupled to GC × GC for the identification of the resolved peaks. The response of a flame ionization detector to different compound classes was calibrated using multiple internal standards. In total, 172 compounds were identified with good match and 61 compounds with high probability value were reliably quantified. For comparative purposes, the essential oil sample was also quantified by one-dimensional gas chromatography-mass spectrometry (GC/MS) with multiple internal standards method. The results showed that there was close agreement between the two analysis methods when the peak purity and match quality in one-dimensional GC/MS are high enough.
GC-MS Characterization of the Compounds in Some Essential Oils
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture, 1970
The composition of different essential oils (menthe, basil, lavender, rose) was investigated by gas chromatographic–mass spectrometry (GC–MS) method to identify those compounds responsible for the characteristic, pleasant floral or aroma odour or taste of some valuable oils. Three different rose oils presented monoterpenes as fragrance target compounds and some aliphatic hydrocarbons with fixative effects responsible for a longer-lasting odour impression. Chromatography was performed on a 5% phenyl methylpolysiloxane column (15 or 30 m x 0.25 mm I.D., 0.25 µm) operated in suitable temperature programs.