Antioxidant and Hepatoprotective Effects of Polygonum bistorta Linn. and Tannic Acid on Carbon Tetrachloride-treated Rats (original) (raw)
Related papers
2013
The leaves of Artemisia princeps Pamp have been used for tea and food in Japan. The polyphenols of the leaves have inhibitory effects against lipid peroxidation and protein fragmentation by free radicals in vitro and an inhibitory effect on galactosaminelipopolysaccharide induced hepatotoxicity in vivo. The levels of serum glutamic oxaloacetic transaminase and glutamic pyruvic transaminase, lipid peroxidation in serum and liver by hepatotoxity were depressed by polyphenols in A. princeps Pamp. The depression of gluthathione and superoxide dismutase in plasma and liver by hepatotoxity were elevated by polyphenols in A. princeps Pamp. These results demonstrated that polyphenols in A. princeps Pamp have antioxidant and hepatoprotective effects.
The aim of this analysis was to see whether the aqueous extract of the roots of Polygonum persicaria (PP) and its active principle, Tannic Acid (TA), had a hepatoprotective and antioxidant effect in rats provided Carbon tetrachloride (1.5 ml/kg, i.p). Twenty albino wistar rats were divided into five groups: control, CCl4-induced hepatotoxicity, hepatotoxicity with Polygonum persicaria and Tannic acid, and a normal group given 100 mg/kg silymarin. After 14 days, the rats were sacrificed. Toxicity testing was carried out on 12 rats. They were randomly allocated to one of three groups: control, Polygonum persicaria 200 mg/kg (B.wt), and Tannic acid 200 mg/kg (B.wt). The amounts of liver homogenate enzymes (glutathione peroxidase, glutathione-S-transferase, glucose-6-phosphatase dehydrogenase, and glutathione reductase enzymes) were greatly restored by extracts of PP and TA at the tested concentrations, supporting the biochemical results. Tannic acid, in contrast to Polygonum persicaria, tends to have a greater liver defensive role toward carbon tetrachlorideinduced hepatotoxicity, as well as antioxidant properties and mild anticancer activity against cell viability at higher concentrations. The histological alterations in the liver indicated the injury. Polygonum persicaria & its active principle Tannic acid has strong antioxidant properties as well as hepatoprotective effects against CCl4-induced hepatotoxicity, as demonstrated by these observations.
Various studies involved in hepatotoxic effect of CCl 4 (1.5 ml/kg, i.p.) is oxidative damage through free radical generation. Drug-induced hepatotoxicity is still a significant unresolved clinical problem as liver is the most common site of damage. Polygonum Bistorta (PB) is powerful astringent, demulcent, diuretic, febrifuge, laxative, strongly styptic and rich in tannins. The goal of the present work is to evaluate and compare the efficacy of root extract of PB (100 mg/kg), TA (25 mg/kg, p.o.) and Resveratrol (30 mg/kg, p.o.) against toxicants induced damage in liver and kidney. The activities of transaminases, alkaline phosphatase and protein were increased in serum after 48 h days of toxicant administration A significant rise was observed in lipid peroxidation level however reduced glutathione content was decreased. A concomitant fall was observed in the enzymatic activities of adenosine triphosphatase, glucose-6-phosphatase. PB and TA can be used to reduce the hepatorenal damage and may serve as an alternative medicine. Thus it may be concluded that TA (25 mg/kg) were found more effective values towards control.
Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery, 2012
The aim of this study was to investigate the antioxidant and hepatoprotective activity of ethanolic extract of the leaves of Polygonum glabrum in carbon tetrachloride (CCl4) induced hepatotoxicity in rats. METHODS: Rats were pretreated orally for 8 days with the ethanolic leaf extract of P. glabrum (200 and 400 mg/kg) and curcumin (100 mg/kg) as the standard prior to CCl4 (1ml/kg body weight, intraperitonial) intoxication. Hepatoprotective action was assessed based on the decrease in the levels of hepatic serum markers such as serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), total bilirubin (TB), direct bilirubin (DB) and increased total protein levels. Further, liver tissue was subjected to histopathological studies and also assessed for oxidative parameters such as malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels. RESULTS: Pretreatment with curcumin and ethanolic leaf extract of P. glabrum significantly inhibited CCl4 induced increase of SGPT, SGOT, ALP, TB and DB levels in serum and decrease in total protein levels. Significant and dose dependent increase in the hepatic SOD, CAT, GSH activities along with decrease in MDA levels were observed in the curcumin and P. glabrum extract pretreated animals. The liver histological examination also showed lowered damage in the extract pretreated animals. CONCLUSION: The ethanolic leaf extract of P. glabrum exhibited significant hepatoprotective effect which could be attributed to its antioxidant activity.
2013
The present work has been designed to evaluate hepatoprotective&in vivo antioxidant effect of Methanol-Aqueous Extract of whole plant Polygonumglabrumwilld. (MAEP) against carbon tetrachloride(CCl4)induced hepatotoxicity in experimental rats. The levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), serum alkaline phosphatase (SALP), uric acid, total protein and total bilirubin were determined. Antioxidant status in liver was determined by measuring the activity of malonaldehyde (MDA), glutathione (GSH), total thiol and catalase (CAT). Total wet weight and histopathological study of isolated liver specimen was also carried out. . The oral pre-treatment with MAEP (200 and 400 mg/kg) showed significant hepatoprotective activity against CCl4 induced hepatotoxicity by decreasing the activities of serum marker enzymes, bilirubin and lipid peroxidation, and significant increase in the levels of uric acid, GSH, Total thiol, Catalase and p...
Antioxidant and Hepatoprotective Effects of Polygonum Odoratum
2001
Oxidative stress has been implicated in the pathology of a variety of human diseases, such as ischaemic-reperfusion injury, atherosclerosis, diabetes mellitus and hepatic injury. A potential therapeutic intervention may include natural antioxidants 1 . Therefore we examined the antioxidant activity and hepatoprotective effect of Polygonum odoratum Lour. An ethanolic extract of dried whole plant of P. odoratum was used in all investigations. The free radical scavenging activity of the extract of P. odoratum was determined by a method based on the reduction of the stable free radical DPPH (1,1-diphenyl-2-picrylhydrazyl). The extract (0.01-1000 μg/ml) was found to scavenge DPPH in the dose-dependent manner with the maximum scavenging activity of 90.9 ± 1.01 % and IC 50 of 14.5 μg/ml. The scavenging effect of P. odoratum on H 2 O 2 production within the rat white blood cells was investigated by using 2-7-dichlorodihydro fluorescein diacetate. The production of H 2 O 2 was stimulated by...
ijpbs.net
The Poly herbal ethanolic extract (PHEE) was evaluated for its anti hepatotoxic potential against carbon tetrachloride (CCL 4 ) induced hepatic damage in male albino rats. Ethanolic extract from the leaves of Melia azadirachta, seeds of Piper longum and whole plant of Eclipta alba at a dose level of 50 mg/kg body weight was administered orally daily once for 14 days. The substantially reduced levels of enzymatic anti-oxidants such as superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase (GPx), glutathione-stransferase(GST) and glutathione reductase (GR), due to carbon tetra chloride effect were restored to normal. Histopathological examination of liver sections of PHEE treated rats shows the restoration of normal tissues. Vitamin-E at a dose of 100 mg/kg taken as a standard reference also exhibited significant antioxidant activity against CCl 4 induced Hepatotoxicity. The results of this study strongly justified that PHEE has a potent hepatoprotective activity against carbon tetrachloride
Mechanism of Polygonum bistorta and Zingiber roseum Against Toxicity
Journal of Drug Metabolism & Toxicology, 2014
The present study was carried out to observe the hepatoprotective effect and antioxidant activity of the aqueous extract of the roots of Polygonum bistorta (PB) (100 mg/kg) and Zingiber roseum (ZR) (100 mg/kg) in rats treated with carbon tetrachloride (0.15 ml/kg, i.p.). Extract of PB and ZR at the tested doses restored the levels of liver homogenate enzymes (glutathione peroxidase, glutathione-S-transferase, superoxide dismutase and catalase enzymes significantly. The activities of MTT assay significantly recovered the damage towards normal. This study shows that Zingiber roseum has a more liver protective effect in comparison to Polygonum bistorta and against carbon tetrachloride-induced hepatotoxicity and possess antioxidant activities and extracts exhibited moderate anticancer activity towards cell viability at higher concentration.
Evidence-Based Complementary and Alternative Medicine
Oxidative stress is an important pathological mechanism in various liver diseases. Polygonum multiflorum Thunb. (PM) can be used for the treatment of diseases associated with aging, hyperlipidemia, and oxidative stress in traditional Chinese medicine. In this study, we examined the hepatoprotective effects of the ethanolic extract of PM (PME) in in vitro and in vivo models. The PME induced expression of antioxidant-response-element- (ARE-) related genes in HepG2 cells showed a dose-dependent manner. Pretreatment of HepG2 cell with PME suppressed H2O2- and acetaminophen- (APAP-) induced cellular reactive oxygen species (ROS) generation and cytotoxicity. In APAP-induced mouse liver injury, pretreatment with PME also showed ability to increase the survival rate and reduce the severity of liver injury. Treatment with PME attenuated bile duct ligation-induced extrahepatic cholestatic liver injury and further increased multidrug resistance protein 4 (MRP4) and reduced organic anion-transp...