Relationship Among Antibiotic Resistance, Biofilm Formation and lasB Gene in Pseudomonas Aeruginosa Isolated from Burn Patients (original) (raw)
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Records of Pharmaceutical and Biomedical Sciences
Pseudomonas aeruginosa (P. aeruginosa) is considered as the foremost cause of hospital-acquired infections due to its innate and plasmid mediated resistance to multiple antibiotics making it a multi-drug resistant (MDR) pathogen. In our cross-sectional study, one hundred and twenty-six (126) non-duplicate clinical P. aeruginosa isolates were recovered from 450 clinical specimens from burn units in Ismailia Hospitals. The antibiotic sensitivity of strong and moderate biofilm producers isolates was investigated using the disc diffusion method. The isolated bacteria were tested for their ability to form biofilm using a microtiter plate assay. The MPA detected 80% (95 /126) isolates as biofilm producers, 18% (22/126) were strong biofilm producers, 34% (43/126) were moderate biofilm producers, 28% (35/126) were weak biofilm producers and 20% (31/126) non biofilm producers. Susceptibility pattern analysis of biofilm forming P. aeruginosa isolates (95) detected that 60% (68/ 95) were multi-drug resistant isolates (MDR). Resistance to all used antibiotics and multidrug resistance was higher among biofilm producing than non-biofilm producing strains, but the difference was statistically non-significant. The present study confirmed that antimicrobial resistance was more prominent in biofilm-producing P. aeruginosa than in non-biofilm-producers.
Biofilm Formation and Virulence Factors Among Pseudomonas aeruginosa Isolated From Burn Patients
Jundishapur Journal of Microbiology, 2015
Background: Pseudomonas aeruginosa possesses a variety of virulence factors and infections caused by multidrug-resistant P. aeruginosa (MDRPA) in burn patients are a public health problem. Objectives: The aim of this study was to determine the antibiotic resistance pattern, the biofilm formation, the prevalence of MDRPA and two virulence genes (nan1 and exoA) among P. aeruginosa isolated from burn patients. Patients and Methods: A total of 144 isolates of P. aeruginosa were collected from burn patient at the Burn Centre of Tehran, Iran, between March 2013 and July 2013. Antibiotic susceptibility test was performed via agar disk diffusion method. The ability of producing biofilm was examined by crystal violet microtiter plate assay and the prevalence of the exoA and nan1 genes among the isolates was determined by polymerase chain reaction (PCR). Results: A high rate of resistance was seen against ciprofloxacin (93.7%), aztreonam (86.8%), piperacillin (85.4%), ceftazidime (82.6%), amikacin (82%) and imipenem (79.2%). In total, 93.1% of the isolates were characterized as MDRPA. Biofilm formation was seen in 92.4% of the isolates. The prevalence of the exoA and nan1 genes were 75% and 11.8% among the isolates, respectively. Conclusions: The high rate of MDRPA and its ability to produce biofilm is an alarm for public health. The statistical analysis showed that biofilm production in the MDRPA isolates was significantly higher than that in the non-MDRPA isolates (P < 0.001).
Laboratory Medicine, 2010
Background: Pseudomonas aeruginosa is 1 of the major agents of nosocomial infections in burn centers. Our objectives were to determine the genetic diversity of P. aeruginosa isolated from burn patients. Methods: One hundred thirty-one P. aeruginosa isolates were collected from the burn center of Shahid Motahhari Hospital in Tehran. Phenotypic screening for drug susceptibility was performed by disk diffusion method according to Clinical Laboratory Standards Institute guidelines, and genetic diversity of all isolates was determined by the pulsed-field gel electrophoresis (PFGE) technique. Results: Antimicrobial susceptibility testing showed that the majority of P. aeruginosa strains were resistant to ceftizoxime (87%) and aztreonam (80.2%). Pulsed-field gel electrophoresis revealed 11 profiles in which environmental strains were included in PFGE1 and PFGE7 patterns. The major PFGE profile was PFGE1 containing 18 (42.9%) MDR isolates and including an environmental MDR bacterium. Conclusions: Our findings highlighted that further attention needs to be focused on the disinfection of this burn unit.
Indian Journal of Forensic Medicine & Toxicology, 2020
Background: Pseudomonas is a common bacteria found all over the world; in soil,water, and plants, and it is one of the most common pathogens in hospital-acquired infections. Aims: The aims of this study were isolation of P. aeruginosa bacteria from patients with inflammation of burns, Diagnosis and identification of P. aeruginosa using chemical tests and VITEK2 system and alsostudy of antibiotic resistance in P. aeruginosa using the VITEK2 system. Method: (206) swabs were collected from wounds and burns; (139) samples from burns and (67) samples from wounds; from different clinical cases for both sexes and ages (1-70) years, the patients coming and sleeping in Baghdad Teaching Hospital and Burns Hospital in the City of Medicine at Baghdad city; the duration from January to the end of March 2019. Samples were cultured on the variety of culture media (MacConkey agar, Blood agar and Cetrimide agar) in order to obtain the bacterial isolates of P. aeruginosa depending on their phenotypic characteristics.VITEK2 system were used for identification P. aeruginosaand to study their resistance to the antibiotics. Results: Out of the 206 samples, 50 Pseudomonas aeruginosa were isolated from swabs. 31(62%) isolates were isolated from burn and 19 (38%) from wound swabs. The isolates were subjected to a series of biochemical tests as diagnosed with Api 20E; and VITEK2 system to increase confirmation of isolation yield for P. aeruginosa bacteria. The results showed that the majority of isolates were (92%) resistant to Amoxicillin while the isolates differed between sensitive and moderate sensitivity and resistance to other types of antibiotics. Conclusion: The study showed that the percentage of isolation of Pseudomonas aeruginosa bacteria from wound infections is relatively higher than the rate of isolation from burn swabs. Most of Pseudomonas aeruginosa isolates showed high resistance to most types of antibiotics used in the present study, especially the antibiotic Amoxicillin where the resistance rate was 92%.
Pseudomonas Aeruginosa in Burn Infections and Its Antimicrobial Resistance
Journal of Evidence Based Medicine and Healthcare
BACKGROUND Pseudomonas aeruginosa is the main culprit of hospital acquired infection especially in wards like critical care area and burn units. Treatment for burns has been improved greatly but infections with multi-drug resistant (MDR) strains P.aeruginosa remain the main concern for death especially in critical care areas. We wanted to update the antibiotic sensitivity profile of Pseudomonas aeruginosa isolated in burn wounds among the burn patients at a tertiary care hospital. METHODS This is a retrospective study conducted in a tertiary health care center for one year. Data regarding the organism Pseudomonas aeruginosa was collected from the medical records. In this, samples from patients admitted in burn unit were collected and the isolates were identified by conventional phenotypic methods. The antibiotic sensitivity testing of all P. aeruginosa isolates was done using modified Kirby-Bauer's disc diffusion method and the results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. RESULTS Of a total of 116 patients, P. aeruginosa was isolated from 62 (41.3%) samples. Among all the isolates 42 (67.7%) were MDR and 18 (29%) were XDR. And the isolates show maximum resistance to Ticarcillin-Clavulanic Acid (96.77%), followed by Cefepime (93.54%), Levofloxacin (93.54%), Piperacillin (91.94%), Netilmicin (91.94%), Ceftazidime (90.32%), Doripenem (90.32%), Ciprofloxacin (87.1%), Imipenem (87.1%), Meropenem (85.49%), Piperacillin-Tazobactam (83.87%), Gentamicin (58.06%), Aztreonam (51.61%), Tobramycin (50%) and Amikacin (48.4%). CONCLUSIONS The result confirmed the prevalence of MDR strains. Prevention of, dissemination and indiscriminate use of antibiotics is important. Novel infection control practices, hospital antibiotic policy and regular surveillance programmes should be implemented.
Malaysian Journal of Microbiology
Aims: Pseudomonas aeruginosa is an opportunistic pathogen that still develops life threatening infections in patients with immunological system defects like burns. The major problem with this organism is the ability to persist during infections due to its high rate of resistance to many drugs. This study was designed to evaluate the prevalence and drug susceptibility profile of Pseudomonas aeruginosa in patients admitted to a burn unit in a tertiary health facility. Methodology and Result: From 80 selected patients, appropriate clinical specimens from burn sites were collected and processed for the isolation and identification of Pseudomonas aeruginosa.. Also, 78 surveillance samples from various environmental sites and hands impressions of nursing staff in the burn unit were cultured for the isolation of Pseudomonas aeruginosa. Drug susceptibility profile showed high resistance for ceftazidime (40.7%) ciprofloxacin (13.1%) and piperacillin (34.6%); furthermore, low resistance for some antibiotics like imipenem (17.5%) pipereracillin/tazobactam (12.3%) and aztreonam (16%) were obtained. In addition, 8 multiresistant Pseudomonas aeruginosa (MRPA) isolates were recovered from clinical specimens and from environmental samples. Conclusion, significance and impact of study: Evidence of high prevalence of clinical and environmental MRPA reported in this study provides the rationale for strict enforcement of infection prevention protocol to minimize cross transmission of bacterial pathogens in hospital burn units and consequent disease burden arising from MRPA.
TJPRC, 2014
During a period of three months (September 2013 – November 2013), a total of 78 Transport swabs with Amies medium were collected from burned victims, who admitted to Burn Unite in Al-Hilla General Teaching Hospital. As right practice, all Transport swabs were collected before washing of the burned person and cultured the cetrimide agar plates for selective isolation of Pseudomonas aeruginosa isolates. The identification was then confirmed by automated identification VITEK 2 Compact system using VITEK 2GN CARD. Antibiotics susceptibility test was achieved according to CLSI 2012. Genes of some virulence factors like alginate biofilm biosynthesis gene (algD) and type III Secretion Toxin genes (exoS, exoT, exoU, and exoY) were investigated using specific primers sets by conventional PCR. The data displayed that (19.2%) of samples gave positive culture for Pseudomonas aeruginosa. All suspected isolates were confirmed by VITEK 2 Compact system. Antibiotics susceptibility test results revealed that low level of resistance were showed for Ticarcillin, Amikacin, Piperacillin, Tobramycin, Norfloxacin and Ceftazidime in which the resistance percent were 6.6%, 6.6%, 6.6%,13.3%, 13.3% and 13.3% respectively. The resistances to other antibiotics were 26.6% for Netilimicin, 66.6% for Nalidixic acid, 80% for Cefuroxime, 86.6% for Cefoperazone. The results of genotypic investigation of some virulence factors gene revealed that, alginate biofilm biosynthesis gene (algD) were positive for (53.3%). Type III Secretion Toxin genes result were as follow: exoS (80.0%), exoT (26.6%), exoU (86.6%), and exoY(73.3%). As conclusion the results display that, P. aeruginosa isolated from burn infection have the ability to form biofilm which may impair entrance of mechanism of action of many antibiotics or make them need concentration more than the usual to do their work. The aminoglycoside still the best choice for treatment. Many isolates have more than one of type III Secretion Toxin which intensifies their pathogenicity.
Saudi medical journal, 2008
To identify Pseudomonas aeruginosa (P. aeruginosa) from the skin biopsy specimens in burn wound infections by multiplex polymerase chain reaction (M-PCR) and detection of antimicrobial susceptibility of isolates from culture. We conducted this cross-sectional study in 140 patients with wound infections who admitted to the referral burn center of Motahari, Tehran, Iran, during a 12-month period from 2005-2006. Skin biopsy specimens were aseptically taken from each patient, one for PCR and one for bacterial culture. A M-PCR test based on the simultaneous amplification of 2 lipoprotein genes: oprI and oprL, was used to directly detect fluorescent pseudomonades and P. aeruginosa in skin biopsy specimens. The susceptibility of P. aeruginosa isolates to 16 antibiotics was determined using the disc diffusion method. Out of 140 biopsy specimens, M-PCR detected 66 (47.2%) isolates, while culture detected 57 (40.7%) isolates as P. aeruginosa. Positive results for both genes which observed onl...
Acta medica Iranica, 2011
Pseudomonas aeruginosa is an important life-threatening nosocomial pathogen and plays a prominent role in serious infections in burned patients. The current study was undertaken to characterize P. aeruginosa strains isolated from burned patients in Tehran, Iran. The study was conducted in a major burn center in Tehran, Iran in 2007. A total of seventy specimens obtained from different clinical origin with positive culture results for P. aeruginosa were included in the study. Antimicrobial susceptibility test was performed according to the standard CLSI guideline. The relationship between the strains was also determined using antimicrobial drug resistance pattern analysis and plasmid profiling. All strains were multi drug resistant. The percentage of resistance to tested antibiotics was: imipenem 97.5%, amikacin 90%, piperacillin 87.5%, ceftizoxime 72.7%, gentamicin 67.5%, ciprofloxacin 65%, ceftriaxone 60%, and ceftazidime 57.5%. Thirteen resistant phenotypes were recognized, R3 (TE...
Burns, 2003
The serovars and drug susceptibility patterns of 265 isolates of Pseudomonas aeruginosa cultured from burn patients during 2001-2002 at Motahari and Tohid Hospital were determined. Distribution of serovars was different at two hospitals. Most of the isolates at Tohid Hospital belonged to serovar 0:1, but 21 and 13% of them were untypeable or polyagglutinable, respectively. Serovar 0:11 was the most prevalent serovar at Motahari Hospital. All the strains were multi resistant to tetracyclin, carbenicillin, amikacin, ceftazidime, sulfamethoxazol, ciprofloxacin, tobramycin, kanamycin, cefotaxime and gentamicin. Further analysis of the strains by plasmid profiling demonstrated that 95% of the isolates carried two megaplasmids. However, there was not any correlation between the serotyping and presence of plasmids. Changes in the drug susceptibility patterns and -lactamase production of some cured derivatives were observed after the strains lost their plasmids. The emergence of multi-drug resistant strains of P. aeruginosa is a serious concern in burn patients who are hospitalized in Tehran.