Environmental stress-induced bacterial lysis and extracellular DNA release contribute to Campylobacter jejuni biofilm formation (original) (raw)
2017, Applied and environmental microbiology
Campylobacter jejuni is a microaerophilic bacterium and supposed to persist in a biofilm to antagonize the environmental stress. This study investigated the influence of environmental conditions on the formation of C. jejuni biofilm. We report an extracellular DNA (eDNA)-mediated mechanism of biofilm formation in response to aerobic and starvation stress. The eDNA was determined to be a major constitutional material of C. jejuni biofilm and closely associated with bacterial lysis. The deletion mutation on the stress response genes spoT and recA enhanced the aerobic influence by stimulating lysis and increasing eDNA release. Flagella were also involved in biofilm formation, but mainly contributed to attachment rather than induction of lysis. The addition of genomic DNA from either Campylobacter or Salmonella resulted in a concentration-dependent stimulation effect on biofilm formation but not due to forming a pre-coating DNA layer. Enzymatic degradation of DNA by DNase I disrupted C....
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