Screening of thirteen medicinal plant extracts for antioxidant activity (original) (raw)
Related papers
Screening of antioxidant activity and phenolic content of 24 medicinal plant extracts
DARU Journal of Pharmaceutical …, 2008
Background: Antioxidants are vital substances which possess the ability to protect the body from damages caused by free radical-induced oxidative stress. A variety of free radical scavenging antioxidants are found in dietary sources like fruits, vegetables and tea. The purpose of this study was to evaluate the antioxidant activity of methanolic extracts of 24 selected plant materials (seeds or fruits), which are used by Iranian people as folk remedies and/or food supplements. Methods: The antioxidant activity was evaluated against linoleic acid peroxidation using 1,3-diethyl-2-thiobarbituric acid as reagent. At the same time the phenolic content of the extracts was determined using Folin-Ciocalteau reagent to evaluate their contribution to total antioxidant activity. Results: The antioxidant activity expressed as IC 50 ranged from 1.25 µg/ml in cucumber to 167.29 µg/ml in cardamom. Phenolic contents, expressed as gallic acid equivalents, varied from 21.76 mg/100g of the dried weight in linseed to 919.12 mg/100 g of the dried weight in Bishop's weed. No significant correlation was observed between antioxidant activity and phenolic content in the studied plant materials. Conclusion: The results of this study showed that there is no significant correlation between antioxidant activity and phenolic content of the studied plant materials and phenolic content could not be a good indicator of antioxidant capacity.
Antioxidant and Free Radical Scavenging Activity of Certain Dietary and Medicinal Plant Extracts
The antioxidant and free radical scavenging activity of the ethanol extracts of Melia dubia Hiern., Albizia lebbeck (L.) Benth., Brassica oleracea L. var. botrytis (broccoli) and Glycyrrhiza glabra L. were evaluated. The total phenolic content of the extracts was found to be higher in A. lebbeck (33.6%) and lower in broccoli (16.9%). The antioxidant activity of the extract was evaluated through different chemical assays and in vitro methods such as reducing power, DPPH x , ABTS x+ and x OH radical scavenging activities, peroxidation inhibition in linoleic acid emulsion system and antihemolytic activity. The increasing concentrations of the extract exhibited nonlinear increases in the reducing power. Extract of A. lebbeck was found to have more hydrogen donating ability. Similar line of dose dependant activity has been observed in DPPH x and x OH radical scavenging systems. Further, A. lebbeck extracts exhibited strong antioxidant activity equivalent to trolox (6423.04 μmol g-1) in ABTS radical cation scavenging system. All the extracts exhibited notable activity against linoleic acid emulsion system (35.5 to 40.2%) and antihemolytic activity (29.8 to 46.6%).
Screening of the Antioxidant Activity of Some Medicinal Plants
Korean Journal of Food Science and Technology, 2004
Antioxidant activities of water extracts of 20 medicinal plants (1 mg/mL) on peroxidation of linolic acid were evaluated by thiocyanate method, among which 11 showed strong antioxidant activity (> 70%). Higher hydroxy radical scavenging activity (> 60%) were shown in Cornus officinalis, Acanthopanax sessiliflorus, and Epimedium koreanum than the other plants. Epimedium koreanum than the other plants extract showed highest superoxide radical scavenging activity (42%). Total polyphenol contents ranged from 2.6 (Polygonatum odoratum)~81.2 (Epimedium koreanum) mg/g. Direct correlation between the antioxidant activity and polyphenol content (r=0.8) was established through simple regression analysis. IC 50 for selected four plant extracts, showing highest polyphenol contents and antioxidant activities, were significantly higher than positive control. Total antioxidant activity of vitamin c was significantly lower than those of Acanthopanax sessiliflorus, Epimedium koreanum, and Erythrina variegata. Superoxide radical scavenging activity of Acanthopanax sessiliflorus was similar to BHA. Results suggest water extracts of some medicinal plants could be potential candidates for natural antioxidants.
2018
Present investigation entitled Studies on superoxide anion radical scavenging activity, antioxidant activity, and reducing power of methanolic and aqueous extract of different medicinal plants was carried out during December 2016 April 2017 in Department of Biochemistry and Molecular Biology, MGM College of Agricultural Biotechnology, Gandheli, Aurangabad. Experiment was laid out in Factorial Randomized Block Design (FRBD) with ten treatments of different medicinal plants (Amla, amsul, chinch-Imli, neem, gavti chahaa, pipal, umber, wad, Pudina, coriender) and three replications. The healthy disease free medicinal plants were selected. The antioxidant activity of the medicinal plants extract and the standard were assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl-2-picryl-hydrazyl (DPPH) free radical activity by modified method. The dilute working solutions of the test extract were prepared in methanol. Ascorbic acid was used as standard in 1100μg/ml so...
study on the antioxidant property of selected medicinal plants
The Clitoria ternatea, Solanum nigrum and Aloe vera leaves were extracted serially by the solvents of increasing polarity (petroleum ether, chloroform and ethanol) were tested for their free radical scavenging activity against DPPH. The petroleum ether extracts of Clitoria ternatea, Solanum nigrum and Aloe vera leaves were the most effective scavenging of DPPH. These plant extracts were also analyzed for the activities of selected enzymic antioxidants such as catalase, polyphenol oxidase and the non-enzymic antioxidants were ascorbic acid and total phenols. The results showed the leaves of Solanum nigrum possess higher levels of antioxidants.
Screening of various botanical extracts for antioxidant activity using DPPH free radical method
African Journal of Traditional, Complementary and Alternative Medicines, 2013
Aiming at the exploration of herbal use by society, crude extracts of the seeds of some commonly used medicinal plants (Vitis vinifera, Tamarindus indica and Glycin max) were screened for their free radical scavenging properties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. The overall antioxidant activity of grape seeds (Vitis vinifera) was the strongest, followed in descending order by soybean (Glycin max) and tamarind (Tamarindus indica). The seeds extract of Vitis vinifera, Glycin max and Tamarindus indica showed 85.61%, 83.45% and 79.26%, DPPH scavenging activity respectively.
Oxidants and Antioxidants in Medical Science, 2014
Objective: Reactive oxygen has been implicated in degenerative diseases and medicinal activity of most herbs has been attributed to their ability to scavenge free radicals. Methods: Free radical scavenging and antioxidant activities of ethanolic extracts from the leaves of varieties of medicinal plants, namely Azadiratcha indica, Mangifera indica, Moringa oleifera, Psidium guajava, Terminalia catappa, Anacardiaceae occidentale, Cassia siamae, Chromolaena odorata, Telfaira occidentalis and Paraquetina nigresiens were evaluated. Reducing power, DPPH scavenging, hydroxyl radical scavenging and ferrous ion chelating activities, phenolic, flavonoid and vitamin C contents were determined. Results: C.siamae had excellent DPPH scavenging activity while P.guajava presented the lowest value. T.catappa had peak hydroxyl radical scavenging activity whereas T.occidentalis had the least capacity. C.siamae had the highest total phenolic and flavonoid contents while P.guajava and P.nigresiens showed the lowest phenol and flavonoid values. C.siamae also exhibited the highest reducing power activity whereas P.nigresiens had the least value. P.nigresiens had excellent ferrous iron chelating capacity while C.odorata had only poor activity. The total phenolic and flavonoid contents were highly correlated with the DPPH scavenging and reducing power activities, respectively. M.oleifera had the highest vitamin C content while P.guajava was the least.. Conclusion: Different values were obtained for each parameter for the medicinal leaves and free radical scavenging activity could be attributed to total phenolic and total flavonoid content. Among the plants tested, Cassia siamae leaf extract consistently exhibited the highest antioxidant activity and seems to be a promising source of natural antioxidants.
Journal of Intercultural Ethnopharmacology, 2018
Background/Aim: The medicinal plants and their derivatives have long been recognized as important sources of antioxidants in the prevention and treatment of various diseases. This study investigated phytochemicals, antioxidant content and in vitro DPPH free radical scavenging activities of methanolic and aqueous leaves extracts of Anogeissus leiocarpus, Ipomoea asarifolia, Bauhinia rufescens, Guiera. senegalensis and Moringa oleifera. Materials and Methods: The extracts were subjected to qualitative phytochemical analysis to identify the bioactive constituents in each plant. Total phenolics and proanthocyanidin contents were determined using FolinCiocalteu and vanillin-methanol assays while antioxidant free scavenging activity was estimated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Results: The phytochemical screening revealed the presence of alkaloids, flavonoids, and tannins in all the five plants investigated. The total phenolic and proanthocyanidin contents of methanoli...
Screening of plant extracts for antioxidant properties
2011
Antioxidant properties of total methanol extracts from 54 species of 30 families were studied. DPPH (1,1-diphenyl-2-picryl hydrazyl) radical was used for evaluation of free radical scavenging. Among tested species, the extracts of Rumex crispus (radix), Rubus occidentalis (folia), Rumex alpinus (radix), Euphorbia helioscopia (herba) and Rubus idaeus (folia), exhibited the strongest activity. Signifi cant radical scavenging activity was found also in the extracts of Echium vulgare (herba), Plantago arenaria (herba), Viola tricolor (folia), Pyrus communis (folia), Sideritis montana (folia), Betula pendula (folia), Achillea critmifolia (herba), Santolina rosmarinifolia (herba), Morus alba (folia) and Erigeron canadensis (herba). Moderate activity was shown by extracts of Forsythia (folia), Bryonia alba (folia), Hepatica nobilis (folia), Plantago cornuti (folia), Maclura cochinchinensis (folia), Cihorium inhybus (herba) and Caltha palustris (herba).