Novel Sensors for Batch and Flow Injection Analysis of Histamine Based on Crown Ethers (original) (raw)
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In this work the implementation of a histamine ion-selective electrode for fish freshness control is described. The solid-contact electrode is based on a polymeric membrane incorporating, respectively, a-cyclodextrin as an ionophore, 2-fluorophenyl 2-nitrophenyl ether as a plasticiser and potassium tetra-kis(p-chlorophenyl) borate as an ionic additive. The conventionally shaped histamine electrode responded to histamine cation in the pH operational range of 3.5–5.5 with a slope of 31.7 ± 1.3 mV dec À1 , and with a practical detection limit of (1.6 ± 0.2) Â 10 À6 mol L À1. The miniaturisation of the above-described electrode enabled its use as a detector in a sequential-injection lab-on-valve system, yet with a useful lifetime shortened from 10 months to approximately 1 month under continuous operation. The optimised flow conditions were achieved for sample injection volumes of 70 lL propelled towards the detection cell at the flow-rate of 12 lL s À1 during 20 s followed by a flow-rate of 15 lL s À1 during 50 s. The potentiometric analysis of histamine in different kinds of fish furnished results similar to those provided by the chromatographic method. The low cost of the analysis, the speed of the method and the use of a smaller volume and non pollutant reagents justify the use of potentiometry as an alternative analytical technique for this food control.
Simulation and Performance Optimization of an Amperometric Histamine Detection System
EUROSENSORS 2018, 2018
One of the most widely known biogenic amines is histamine, which plays an important role in the human immune system. Some people suffer from allergic reactions after a histamine-rich diet; this is called histamine intolerance. The aim of this work is to develop a quick and reliable method for the detection and quantification of histamine in food, based on an electrochemical approach. In presence of biogenic amines, a reduction cascade induces a current at the working electrode. Prior to chronoamperometric measurements, Finite Elemente simulations were performed. The results are presented in this work.
Analytical Chemistry, 2013
Histamine is a biogenic amine that is indispensable in the efficient functioning of various physiological systems. In previous work, a molecularly imprinted polymer (MIP) based sensor platform with impedimetric readout was presented which could rapidly and at low cost determine histamine concentrations in buffer solutions within pH 7−9. 1,2 For diagnostic applications, histamine should be detectable in a wider pH range as it mostly occurs in mildly acidic environments. To understand this pH-dependent response of the MIP sensor, we propose a statistical binding analysis model. Within this model, we predict the theoretical performance of MIP based on acrylic acid in the required pH range and verify these results experimentally by UV−vis spectroscopy, microgravimetry, and impedance spectroscopy. Using impedimetric read-out, specific and selective detection of histamine in the physiologically relevant nanomolar concentration range is possible in neutral and mildly acidic phosphate buffer. Finally, this sensor platform was used to analyze the histamine concentration of mildly acidic bowel fluid samples of several test persons. We show that this sensor provides reliable data in the relevant concentration regime, which was validated independently by enzyme-linked immuno sorbent assay (ELISA) tests.
Sensors and Actuators B: Chemical, 2010
The need for more advanced, accurate and lower cost sensor platforms is constantly growing. However, for certain applications the already existing sensing systems based on biological recognition elements have sometimes restrictions, which limit their use. As a result, sensors with synthetic recognition elements, such as molecular imprinted polymers (MIPs), can be interesting alternatives. Molecular imprinting leads to the formation of inert polymer particles with nanocavities, which can exhibit similar selectivity and specificity to target molecules as antibodies or enzymes. It is demonstrated that MIPs can be readily incorporated into two different sensor platforms for the detection of histamine in aqueous media. The first platform is based on electrochemical impedance spectroscopy and allows for the accurate detection of histamine in the nanomolar range. The second sensing technique is based on microgravimetry and allows for the detection of histamine in the micromolar range. Using the analogous molecule histidine, it is demonstrated that both sensor platforms are specific for the detection of histamine.
Analytical Biochemistry, 2013
An electrochemical detection (ECD) method for analyzing sub-micro amounts of histamine (HA) and N smethylhistamine (N s-MHA) in biological samples by high-performance liquid chromatography (HPLC)amperometry has been developed. The method consists of a precolumn derivatization of the amines with o-phthalaldehyde (OPA) and sodium sulfite (Na 2 SO 3) to N-alkyl-1-isoindole sulfonate and posterior separation with the HPLC system. Biological samples were pretreated by using a Vivapure sulfonic acid minifilter in which the reaction of the reagent with the amines took place during filtering. HA and N s-MHA retention times were 11.8 ± 0.02 and 18.3 ± 0.03 min, respectively (means ± standard deviations, n = 3). The lowest limit of amperometric detection at a signal-to-noise ratio of 3:1 was 0.125 pmol in both cases. HA and N s-MHA contents in hypothalamus, cortex, skin, and fundic gland, as well as histamine N-methyltransferase (HMT) activities of mast cell-deficient (Ws/Ws) and Wistar rats, were measured and compared with an HPLC-fluorometry system, among other experiments, in order to validate and demonstrate the usefulness of this assay system. Hence, this consequently confirms not only the sensitivity and specificity of the assay but also the potential and convenience it offers to laboratory work, especially in the analysis of the regulation of histaminergic neurons as well as enzymatic investigation of HA metabolism.
Ultratrace Detection of Histamine Using a Molecularly-Imprinted Polymer-Based Voltammetric Sensor
Rapid and cost-effective analysis of histamine, in food, environmental, and diagnostics research has been of interest recently. However, for certain applications, the already-existing biological receptor-based sensing methods have usage limits in terms of stability and costs. As a result, robust and cost-effective imprinted polymeric receptors can be the best alternative. In the present work, molecularly-imprinted polymers (MIPs) for histamine were synthesized using methacrylic acid in chloroform and acetonitrile as two different porogens. The binding affinity of the MIPs with histamine was evaluated in aqueous media. MIPs synthesized in chloroform displayed better imprinting properties for histamine. We demonstrate here histamine MIPs incorporated into a carbon paste (CP) electrode as a MIP-CP electrode sensor platforms for detection of histamine. This simple sensor format allows accurate determination of histamine in the sub-nanomolar range using an electrochemical method. The sensor exhibited two distinct linear response ranges of 1 × 10 −10 –7 × 10 −9 M and 7 × 10 −9 –4 × 10 −7 M. The detection limit of the sensor was calculated equal to 7.4 × 10 −11 M. The specificity of the proposed electrode for histamine is demonstrated by using the analogous molecules and other neurotransmitters such as serotonin, dopamine, etc. The MIP sensor was investigated with success on spiked serum samples. The easy preparation, simple procedure, and low production cost make the MIP sensor attractive for selective and sensitive detection of analytes, even in less-equipped laboratories with minimal training.
Study of Histamine Detection using Liquid Chromatography and Gas Chromatography
ASM science journal, 2021
Histamine is a heterocyclic amine shaped by decarboxylation of the histidine. It is a compound that lack chromophore and involatile. However, the detection of histamine is imperative due to the characteristic of histamine has given several disadvantages in food industry. This paper describes methods for histamine detection by employing high performance liquid chromatography and gas chromatography. The derivatization techniques required for both methods in order to increase the sensitivity of chromatography analysis. Two derivatizing agents were applied in this study such as 9-flourenilmethyl chloroformate (FMOC-Cl) for HPLC analysis whereas for GC analysis a N,O-bis (trimethylsilyl)acetamide (BSA) was used. Method validation was in accordance to Commission Decision 657/2002/CE. The validation of specificity, linearity, precision, accuracy, detection limit and quantitation limit results indicate that the methods were acceptable. The linear range for both methods were at 0.16-5.00 µg•mL-1. The determination of histamine using GC showed the superiority of this instrument compared to HPLC. Method applicability was also checked on real sample namely mackerel in order to acquire a satisfactory recovery for both methods.
Study of the Electrochemical Behavior of Histamine Using a
2015
A study on the electrochemical behavior of histamine species in aqueous medium is described. A glassy carbon electrode chemically modified with copper(II) hexacyanoferrate (CuHCFe) film and covered with Nafion film was employed. The interaction between the analyte and the CuHCFe film can be demonstrated by a decrease in both the cathodic and anodic peak currents at 0.68V (vs. Ag/AgCl), attributed to the film and the appearance of new peak current at 0.47V. Cyclic voltammetric parameters obtained for histamine indicate the formation of stable complex between histamine adsorbed at the electrode surface. The dependence of peak currents on the concentration of the analyte is not linear in the employed work range, indicating the presence of a coupled chemical reaction in the electrodic process.