A validated RP-HPLC method for quantitation of trigonelline from herbal formulations containing Trigonella foenum-graecum (L.) seeds (original) (raw)
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Iranian Journal of Pharmaceutical Research : IJPR, 2020
Mathematical algorithms offer a useful method for quantitative analysis of compounds in multi-component mixtures to overcome the overlapping problems occurred in UV spectrophotometry. The aim of this study is to develop a method for simultaneous determination of bioactive compounds in herbal dosage forms produced from fenugreek extract. A UV- spectrophotometric method based on mathematical algorithm was used to simultaneous determination of trigonelline (TRG), diosgenin (DI), and nicotinic acid (NA). The maximum absorbance (λmax) was determined to be 232.65 nm, 296.23 nm, and 262.60 nm for TRG, DI, and NA, respectively. The calibration curves showed good linearity for all analytes in the concentration range of 1–20 μg/mL (R2=0.9995, 0.9997, 0.9994 for TRG, DI and NA, respectively). The Intra- and inter-day precisions were in the range of 1.1-10.7% and 1.2-8.2%, respectively. The accuracy of the method was 96.0% for TRG, 92.9% for DI, and 104.2% for NA. The limits of detection (LOD) ...
Analysis of Phytoconstituent Profile of Fenugreek – Trigonella Foenuem-Graecum L. – Seed Extracts
Studia Universitatis Babeș-Bolyai Chemia, 2017
Fenugreek (Trigonella foenum-graecum L.) is a well-known herb for its efficiency in the prevention/treatment of diabetes among other chronic diseases. The aim of present study was to analyse the phytoconstituent profile of aqueous and hydro-alcoholic extracts of fenugreek seeds produced in Hungary. The aqueous and hydro-aqueous extracts were analysed using a UHPLC-ESI-MS approach, and in the first 54, while in the second extract 67 phytoconstituents were identified that mostly corroborate the previously described health promoting effects of fenugreek. However, it remains a huge challenge to correlate the phytoconstituent composition of the two extracts with the generated dose dependent hormetic response and cytotoxic effects that were reported by us in case of some human breast cancerous cell lines.
Improvement in the nutraceutical properties of fenugreek ( Trigonella foenum-graecum L . )
2006
Acharya, S., Srichamroen, A., Basu, S., Ooraikul, B. and Basu, T. Improvement in the nutraceutical properties of fenugreek (Trigonella foenum-graecum L.) Songklanakarin J. Sci. Technol., 2006, 28(Suppl. 1) : 1-9 Fenugreek (Trigonella foenum-graecum L.) leaves and seeds have been used extensively for medicinal purposes. Fenugreek seed is known to exhibit anti-diabetic and anti-nociceptive properties and effects such as hypocholesterolaemic, anti-cancer and thyroxine-induced hyperglycaemia. Our research objectives have been to identify the chemical constituent(s) responsible for the health effects in human and to develop a strategy for improving these constituents in fenugreek plants. We have observed considerable variability among fenugreek genotypes. They differ in morphology, growth habit, biomass and seed production capability. Chemical constituents of the seed, e.g. saponins, fibre, protein, amino acids and fatty acid contents also differ markedly. This variability is most often ...
HPLC ANALYSIS OF TRIGONELLA FOENUM-GRAECUM SEEDS TO ASSESS PHYTOESTROGENS
Phytoestrogens are compounds synthesized in plants which mimic steroidal estrogen activity in mammals. Trigonella foenum-graecum (L.) (Fabaceae, Fenugreek) is employed in many traditional systems as an antibacterial and antidiabetic agent, gastric stimulant and galactogogue and also shows estrogenic, antidiabetic and anti-invasive activity. The present study report the separation of phytoetrogenic components of three different classes – isoflavonoids (genistein and daidzein), lignans (secoisolaricoresinol, metairesinol) and coumestrol from fenugreek seeds before and after alkaline hydrolysis. For this an HPLC system with a C-18 analytical column (100 x 4.6 mm , 5 micron) and detector of UV @ 254nm were employed. The mobile phases contained water/acetonitrile/acetic acid. The limit of detection for isoflavones is 1 µg/g, and for lignans and coumestrol 2.5 µg/g. The results pointed the presence of isoflavones (daidzein-18.2ppm, genistein-11.8ppm) and lignans (secoisolariceresinol-283.6ppm) unhydrolysed and presence of isoflavones (daidzein-100.9ppm, genistein-56.1ppm), lignans (secoisolariceresinol-1893ppm) and coumestrol (170ppm) in hydrolysed fenugreek seeds. Alkaline hydrolysis helped in better separation and quantification of phytoestrogens. High pressure liquid chromatography (HPLC) has been found to be an efficient and sensitive method for identification and quantification of different classes of phytoestrogens-isoflavonoids, lignans and coumestrol.
Standardization of Trigonella foenum-graecum L. Seeds: A Quality by Design Approach
Indian Journal of Pharmaceutical Education and Research, 2020
Aim: To standardize Trigonella foenum-graecum L. Seeds by developing QbD based HPLC method for identification and quantification of trigonelline in T. foenum graecum L. seeds, along with evaluation of various quality control parameters. Methods: The Analytical Target Profile and Critical Quality Attributes were determined followed by optimization of HPLC method by using 2 2 factorial design for designing the experiments for selected independent factors. Method Operable Design Region was developed for finding out the optimized chromatographic conditions. Further quality control parameters such as macroscopic and microscopic characters, physicochemical and phytochemical characterization including determination of toxic elements were carried out on the herb. Results: By application of QbD approach the optimized mobile phase was identified as water with 0.01% Hydrochloric acid and Methanol in the ratio of 70:30, with the flow rate of 1 mL/ min and UV detection at 263 nm. The linear model was established in the range of 2-10µg/mL with R 2 value 0.998. The retention time of Trigonelline was found to be 2.877 min and the amount of Trigonelline in T. foenum-graecum L. Seeds was found to be 0.58%. The inter-day and intra-day precision were less than 2%, with accuracies between 96.6-110% of the true values. The quality control parameters showed the results within specified limits and the seeds showed absence of toxic elements in it. Conclusion: From the above finding we can conclude that the application of QbD approach for standardization of herbal drug can serve as an important tool for development of herbal drugs with desired quality.
2022
The impact of household processes on fenugreek leaves and seeds was analyzed for total phenolic (TP) and total avonoid content (TF), and in-vitro biological activities such as antioxidant, antimicrobial, and anti-in ammatory properties. Processes included air-drying of leaves and germinating, soaking, and boiling of seeds. Air-dried fenugreek leaves (ADFL) had high TP (15.27 mg GAE/g D.W.) and TF (7.71 mg QE/g D.W.). The TF of unprocessed, germinated, soaked, and boiled seeds had 6.54, 5.60, 4.59, and 3.84 mg GAE/g D.W., respectively. The TF in UFS, GFS, SFS, and BFS were 4.23, 2.11, 2.10, and 2.33 mg QE/g D.W., respectively. Sixteen phenolic and nineteen avonoid compounds were identi ed using the HPLC. Antioxidant activity using DPPH • , ABTS +• , and FRAP • assays indicated that ADFL had high activity. Antimicrobial activity was evaluated against each eight pathogenic bacterial and fungal strains. ADFL showed a strong activity with MIC values ranging from 0.03 to 1.06 and 0.04 to 1.18 mg ml − 1 against bacterial and fungal strains, respectively. Anti-in ammatory activity was evaluated in-vitro against RAW 264.7 macrophage cells using of NO assay. Results revealed that ADFL had the highest cytotoxicity and antiin ammatory activity according to NO assay. Household processes signi cantly declined the in-vitro biological properties of processed seeds.
Screening and Biochemical Quantification of Phytochemicals in Fenugreek
The preliminary phytochemical analysis and its quantification were performed in leaves, stem and seeds of different extracts in Trigonella foenum-graecum. From the observation, the Green leafy vegetable (GLV) was good with regards to phytochemicals. GLV have considerable amount of carbohydrates, phenols, sterols, saponins, quinones, alkaloids, terpenoids and tannins. On the contrary slight presence was reported for proteins, glycosides, flavonoids, leucoanthocyanidines in the GLV and phytochemicals like volatile oils, catechol, cyanogenic glycosides, anthocyanin and lignin were absent. The study was further extended to quantify some of the biochemical constituents like carbohydrates, proteins, chlorophyll and carotenoids in which all revealed it’s most significant presence. Overall, from the findings of this study it could be concluded that the selected GLV are immense source of phytochemicals, thus validate this GLV to encourage eating them every day.
Improvement in the nutraceutical properties of fenugreek (Trigonella foenum-graecum L.) Songklanakarin J. Sci. Technol., 2006, 28(Suppl. 1) : 1-9 Fenugreek (Trigonella foenum-graecum L.) leaves and seeds have been used extensively for medicinal purposes. Fenugreek seed is known to exhibit anti-diabetic and anti-nociceptive properties and effects such as hypocholesterolaemic, anti-cancer and thyroxine-induced hyperglycaemia. Our research objectives have been to identify the chemical constituent(s) responsible for the health effects in human and to develop a strategy for improving these constituents in fenugreek plants. We have observed considerable variability among fenugreek genotypes. They differ in morphology, growth habit, biomass and seed production capability. Chemical constituents of the seed, e.g. saponins, fibre, protein, amino acids and fatty acid contents also differ markedly. This variability is most often overlooked or underestimated in clinical trials. Our research sugg...
Annals of Phytomedicine: An International Journal, 2021
Fenugreek (Trigonella foenum-graecum L.), popularly known as methi, is a Leguminosae plant with many pharmacological and pharmaceutical benefits. Traditionally, it is used in Ayurveda, Chinese and Arabic systems of medicine. Fenugreek leaves, seeds, and oil has the potential to cure a variety of malady or illness such as gastric ulcer, gout, epilepsy, piles, chronic cough, bronchitis, and arthritis. Some research findings suggest that fenugreek seeds possess antioxidant, anti-inflammatory, antipyretic, antitumor, antimicrobial, and antidiabetic activity. Fenugreek seeds contain various essential phytoconstituents which are responsible for the pharmacological effects. The goal of this study is to explain the preliminary phytochemical standard of fenugreek seeds with powder microscopy and LCMS analysis.
International Journal of Current Microbiology and Applied Sciences, 2018
Fenugreek (Trigonella foenum-graecum L.) is one of the oldest crops of the world and grown for its medicinal values. It is a self-pollinated diploid species with chromosome number of 2n = 16 (Frayer, 1930). The fenugreek, also known as “Methi”, is an important leafy vegetable cum seed spices belongs to the family Fabaceae. Fenugreek is mainly cultivated in India, Argentina, Egypt, Morocco, Southern France, Algeria, Ethiopia and Lebanon (Bose et al., 1993). In India it is mainly cultivated in Rajasthan, Gujarat, Tamil Nadu, Andhra Pradesh, Uttar Pradesh, Himachal Pradesh and Haryana with total area of 247000 hectares with production of 219000 metric tonnes (Anonymous, 2015-16a). Plant extracts are known for their ethno medical traditions, that is they possess various pharmaceutical and nutraceutical properties. Fenugreek is one such plant that possesses phytoconstituents such as flavonoids, alkaloids, terpenoids, steroids, saponins, anthocyanin and tannins (Sumayya et al., 2012). Wit...