Longitudinal analysis of the effects of IGF1-SnaBI genotypes on the growth curve of Angus bull calves (original) (raw)
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Relationship between blood plasma IGF-1 and GH concentrations and growth of Holstein steers
… de Producción Animal, 2011
Insulin-like growth factor-1 (IGF-1) and growth hormone (GH) have been studied as indicators of growth potential in beef cattle, but these relationships had not been studied previously in Holstein steers. In this case 12 calves weaned at a mean age of 45 d and body weight (BW) of 54.6 kg were used to obtain, every 28 d during a 336-day experiment, BW data and blood samples. Ten blood samples collected at 30-minute intervals, from 0800 to 1300 h were mixed together and analyzed for concentration of IGF-1 and GH by radio-immuno assay. Linear regression and correlation analyses were performed to determine the relationship between average daily gain (ADG) and BW, and plasma IGF-1 and GH concentrations. The correlations between plasma IGF-1 and ADG or BW were consistently positive (0.47 and 0.48), but those between GH and ADG or BW were negative (-0.31 and-0.37, respectively). Plasma concentration of IGF-1 explained 24% of the variation in ADG, but GH explained only about 10%. Concentration of IGF-1 showed a strong relationship with BW (R 2 = 0.41) throughout a 336-day period of growth. These data indicate that plasma IGF-1 may be useful for predicting performance of Holstein steers.
Association of GH and IGF-1 polymorphisms with growth traits in a synthetic beef cattle breed
Genetics and Molecular Biology, 2005
The Canchim beef cattle (5/8 Charolais + 3/8 Zebu) has been selected for meat production in Brazil since late 1950. In the present work the effects of growth hormone (GH) and insulin-like growth factor 1 (IGF-1) polymorphisms were investigated in 688 animals born between 1998 and 2000. These animals belonged to two genetic groups, i.e., traditional and new lineages. Genotype effects on expected breeding values for birth weight (BW), weaning weight (WW) and yearling weight (YW) were investigated by the least square method. Significant effects were found for GH genotype on YW (p ≤ 0.05), with positive effects associated with the LV (leucine/valine) genotype. For IGF-1 genotypes, significant effects were found on BW (p ≤ 0.01) and YW (p ≤ 0.01). Average substitution effects for IGF-1 alleles estimated by regression analysis suggested a positive effect of the IGF-1 225 bp allele on BW and of the 229 bp allele on YW.
British Poultry Science, 2015
Aim: This study aimed to identify the effect of the insulin-like growth factor 1 (IGF1) gene on growth, to uncover the genetic marker at the IGF1 gene, and to predict growth performance by analyzing growth models of Kejobong goats based on their genotype. Materials and Methods: DNA and records of body weight (BW) and body measurements (BM) of Kejobong goats were collected, the IGF1 gene was amplified from the DNA template by polymerase chain reaction (PCR); the PCR products were then sequenced to determine single nucleotide polymorphisms (SNP). Linear mixed model (LMM) was used to analyze the association between SNP and growth traits. Four non-linear growth models were analyzed using non-LMM to describe the growth model and to compare the growth within genotypes. Results: An SNP at intron 4 (g5752G→C) genotyped into GG and CC was significantly associated with BW and BM. Goats of genotype GG had a significantly higher BW and BM (p<0.05) than those of genotype CC. Growth analysis showed that the von Bertalanffy model was the most fit for describing BW, the Brody model for chest width and hip height, the Gompertz and Logistic models for heart girth, and the von Bertalanffy and Gompertz models for hip width. Conclusion: An SNP at intron 4 of the IGF1 gene was associated with the growth trait and was usable as a genetic marker candidate for improvement of growth traits of Kejobong goats while von Bertalanffy model provides proper and accurate estimates of parameters to describe the growth performance of Kejobong goats.
Journal of Animal and Feed Sciences
The aims of our experiments were (1) to study age-dependent changes in bovine plasma concentrations of insulin-like growth factor I (IGF-1), thyroid hormones (T3, T4), some metabolites (glucose, triglycerides, ketone bodies), calcium, phosphorus and sodium, and (2) to determine whether plasma concentrations of these substances are associated with individual and sex-dependent differences in the growth rates of calves. Eight male and 8 female calves were weighed at the beginning and at the end of the experiment and daily gain and feed consumption (dry matter, crude protein, PDIN and NEL per kg daily gain) were determined. Plasma concentrations of IGF-I, T3, T4, glucose, triglycerides, ketone bodies, calcium, phosphorus and sodium were measured in calves at 2, 4, 6, 8, 10 and 12 weeks of age. There were significant age-dependent increases in the plasma concentrations of IGF-I and glucose. Males had a significantly higher daily gain and lower feed consumption than females, but no sex-dependent differences in plasma constituents were detected. In both sexes, daily gain was positively correlated with plasma IGF-I (coefficients of correlation r=0.630 in females and r=0.614 in males) and glucose (r=0.496 and 0.427, respectively) concentrations, but not with other substances. Our observations show that there are age-dependent changes in IGF-I and glucose concentrations (but not in those of other substances), and that IGF-I and glucose, in contrast with other substances, may be indicators or regulators of individual growth rates.
Animal Genetics, 2008
A population of 1398 Canchim (CA) cattle was genotyped to assess the association of an insulin-like growth factor 1 (IGF1) gene microsatellite with phenotypic variation and estimated breeding values of pre-weaning, weaning and post-weaning growth traits. After an initial analysis, the IGF1 genotype only had a significant effect (P < 0.05) on birth weight (BW) and weaning weight adjusted to 240 days (WW240). For these two traits, direct and maternal breeding values were estimated using the restricted maximum likelihood (REML). Two analyses were carried out. In the first (Model I), all fixed effects were fitted. In the second (Model II), the fixed effect of the IGF1 genotype was omitted. The estimated genetic and phenotypic components of variance were similar for every trait in both models. For Model I, estimated direct and maternal heritabilities were 0.26 and 0.16 for BW and 0.23 and 0.14 for WW240 respectively. The genetic and phenotypic correlations between BW and WW240 were 0.38 and 0.38 (Model I) and 0.19 and 0.38 (Model II) respectively. Fifty animals were classified according to their direct and maternal breeding values for both traits. Spearman rank-order correlation between animal rankings in the two models was used to assess the effect of including the IGF1 genotype in the model. Non-significant values from this correlation were indicative of a difference in breeding value rankings between the two approaches. The IGF1 gene was found to be associated with phenotypic variation and breeding values in the early phase of growth.
Polymorphisms in the IGF1 gene and their effect on growth traits in Mexican beef cattle
Genetics and Molecular Research, 2010
The IGF1 gene (insulin-like growth factor 1) is a candidate gene for marker-assisted selection strategies. A single nucleotide polymorphism in the promoter region (IGF1/SnaBI) has been reported to be associated with production traits in several cattle breeds. Here, we report its allelic frequencies in Charolais and Beefmaster breeds; we confirm its association with three growth traits: weaning weight, weaning weight adjusted to 210 days and preweaning weight gain in the Charolais breed. In addition, we designed a strategy to search these breeds for new polymorphisms in four coding regions of the gene. A C/A transversion was detected in intron 4, but it was not associated with the growth traits. A single nucleotide polymorphism (IGF1/SnaBI) is proposed as a selection marker for Mexican Charolais cattle; validation of its association with weaning weight, weaning weight adjusted to 210 days and preweaning weight gain, could complement the genetic evaluations of this breed through marker-assisted management strategies.
Animal Genetics, 2008
A population of 1398 Canchim (CA) cattle was genotyped to assess the association of an insulin-like growth factor 1 (IGF1) gene microsatellite with phenotypic variation and estimated breeding values of pre-weaning, weaning and post-weaning growth traits. After an initial analysis, the IGF1 genotype only had a significant effect (P < 0.05) on birth weight (BW) and weaning weight adjusted to 240 days (WW240). For these two traits, direct and maternal breeding values were estimated using the restricted maximum likelihood (reml). Two analyses were carried out. In the first (Model I), all fixed effects were fitted. In the second (Model II), the fixed effect of the IGF1 genotype was omitted. The estimated genetic and phenotypic components of variance were similar for every trait in both models. For Model I, estimated direct and maternal heritabilities were 0.26 and 0.16 for BW and 0.23 and 0.14 for WW240 respectively. The genetic and phenotypic correlations between BW and WW240 were 0.38 and 0.38 (Model I) and 0.19 and 0.38 (Model II) respectively. Fifty animals were classified according to their direct and maternal breeding values for both traits. Spearman rank-order correlation between animal rankings in the two models was used to assess the effect of including the IGF1 genotype in the model. Non-significant values from this correlation were indicative of a difference in breeding value rankings between the two approaches. The IGF1 gene was found to be associated with phenotypic variation and breeding values in the early phase of growth.
Journal of animal science, 1991
A study was undertaken to determine the effect of selection for high weaning weight on concentrations of plasma insulin-like growth factor I (IGF-I) in sheep and to evaluate the usefulness of measuring IGF-I as an aid in identification of genotypes with a higher growth potential. Lambs from two lines selected for high 120-d weight (HW and DH) and an unselected control (C) were weighed and blood samples collected monthly from birth to weaning (4 mo. of age). A clear differentiation in size occurred after 1 mo of age between lines, between sexes, and between singles and twins. At weaning, selected lines were 3.8 and 5.0 kg heavier than controls. Plasma IGF-I concentrations were 1.5 to 2 times higher (P less than .001) in males than in females after 1 mo of age. There were no significant differences in IGF-I concentration between lines or types of birth. However, line DH and single lambs on average had higher concentrations of IGF-I. Within sex and type of birth correlations between IG...