beta -Galactosidase Enzymatic Activity as a Molecular Probe to Detect Specific Antibodies (original) (raw)
Construction and characterization of a recombinant tripartite enzyme, galactose dehydrogenase/β-galactosidase/galactokinase
Leif Bülow
FEBS Letters, 1990
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Distinct mechanisms of antibody-mediated enzymatic reactivation in β-galactosidase molecular sensors
Jordi Feliu
FEBS Letters, 1998
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Construction of Expression Vectors for Gene Fusions on the Model of ?-Galactosidase-Human Fibroblast ?-Interferon for the Purpose of Immunoenzyme Assay
Alla Lapidus
Annals of the New York Academy of Sciences, 1991
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Antibody-mediated activation of a deletion-mutant β-galactosidase defective in the α region
Roberto Accolla
FEBS Letters, 1976
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Enhanced response to antibody binding in engineered β-galactosidase enzymatic sensors
Neus Ferrer-Miralles
Biochimica Et Biophysica Acta-protein Structure and Molecular Enzymology, 2002
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Affibody-β-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol
Ingmarie Höidén-guthenberg
Journal of Immunological Methods, 2003
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Antibody-Mediated Activation of Genetically Defective Escherichia Coli Galactosidases by Monoclonal Antibodies
Roberto Accolla
Protein Conformation as an Immunological Signal, 1983
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Solubilization and immune-detection of beta-galactosidase hybrid proteins carrying foreign antigenic determinants
Keith Stanley
Nucleic Acids Research
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Enhanced response to antibody binding in engineered �-galactosidase enzymatic sensors
Esther Blanco
Bba Protein Struct Mol Enzym, 2002
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Engineering of Escherichia coli β-galactosidase for solvent display of a functional scFv antibody fragment
Neus Ferrer-Miralles
Febs Letters, 2003
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Advantages and Disadvantages of His-Tagged Beta-Galactosidase
MARIA ANGELICA PERILLO
2020
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The purification of beta-galactosidase from Escherichia coli by affinity chromatography
Harvey Pollard
The Journal of biological chemistry, 1971
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Peptide insertions in β-galactosidase activating interface can improve binding in TPEG-Sepharose affinity chromatography
Jordi Feliu
Biotechnology Techniques, 1995
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Peptide insertions in ?-galactosidase activating interface can improve binding in TPEG-Sepharose affinity chromatography
Jordi Feliu
Biotechnology Techniques, 1995
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Antibody-Mediated Activation of a Defective -D-Galactosidase: II. Immunological Relationship Between the Normal and the Defective Enzyme
john Elis
Journal of Experimental Medicine, 1971
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Engineering Regulable Escherichia coliβ-Galactosidases as Biosensors for Anti-HIV Antibody Detection in Human Sera
Neus Ferrer-Miralles
Journal of Biological Chemistry, 2001
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Antibody-mediated activation of genetically defective Escherichia coli beta-galactosidases by monoclonal antibodies produced by somatic cell hybrids
Roberto Accolla
Proceedings of the National Academy of Sciences of the United States of America, 1981
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Secretion of a hybrid K. lactis-A. niger �-galactosidase
Manuel Becerra
Microb Cell Fact, 2006
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Immunoprecipitation combined with microchip capillary gel electrophoresis: Detection and quantification of β-galactosidase from crude E. coli cell lysate
Martina Marchetti-Deschmann
Biotechnology Journal, 2011
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Preparation of Biotinylated .beta.-Galactosidase Conjugates for Competitive Binding Assays by Posttranslational Modification of Recombinant Proteins
Sylvia Daunert
Analytical Chemistry, 1995
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Physical and kinetic effects on introduction of various linker regions in β-galactosidase/galactose dehydrogenase fusion enzymes
Leif Bülow
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1996
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Chinese hamster ovary cells can produce galactose-α-1, 3-galactose antigens on proteins
Ganesh G
Nature Biotechnology, 2011
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Comparision of the methods available for the purification of hybrid ?-galactosidase proteins produced under the control of an anaerobically-induced promoter
Jeff Cole
Biotechnology Techniques, 1990
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Expression and characterization of a tripartite fusion protein consisting of chimeric IgG-binding receptors and β-galactosidase
Sophia Hober
Journal of Biotechnology, 1990
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Integrated approach to produce a recombinant, His-tagged human α-galactosidase A in mammalian cells
Rosa Mendoza, Julia Lorenzo, Esther Vázquez, José Corchero
Biotechnology progress
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Characterization of a Chemically Conjugated ?-Galactosidase Bioreactor
Harold Swaisgood
Journal of Food Biochemistry, 1993
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Efficient isolation of genes by using antibody probes
Richard A. Young
… Academy of Sciences of the United …, 1983
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Human glutaredoxin 2 affinity tag for recombinant peptide and protein purification
Mathias Lundberg
Protein Expression and Purification, 2006
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Photometric and Electrochemical Enzyme-Multiplied Assay Techniques Using β-Galactosidase as Reporter Enzyme
HAROLD G MONBOUQUETTE
Biotechnology Progress, 2006
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Linker mutagenesis in the lacZ gene of Escherichia coli yields variants of active beta-galactosidase
Wolfgang Kuchinke
European Journal of Biochemistry, 1991
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