Genetic diversity of Turkish lathyrus L. landraces using ISSR markers (original) (raw)
Related papers
Acta agriculturae Slovenica, 2013
Eighteen L. inconspicuous accessions collected from different countries were evaluated for variations of seed weight, seed protein content, and electrophoretic patterns of the total seed proteins analyzed under reducing conditions. They exhibited a reasonable genetic variability for the evaluated traits. This genetic variability revealed that improvement through simple selection for these traits is possible. The variation between the seed size of this accessions was attributed to the development process or the life cycle of the plant, and the environmental condition to which the mother plant is exposed. On the other hand, the variation in protein content among the different accessions may be due to genotype and/or seasonal influences. The relationship between protein content and 100 seeds weight in the evaluated accessions was reversible, the accession showed the lowest quantity of the total seed proteins was the accession that exhibited highest weight of 100 seeds and nearly vice versa. Each accession gave a different electrophoretic pattern except the two accessions collected from Iran, exhibited an identical one. The difference in 100 seed weight and total protein content of these accessions indicated that they are not genetically identical. The variation in the electrophoregram of the evaluated accessions located in the bands with molecular weight more than 98 kDa, the heavy subunits of alpha-lathyrin subunits and the region molecular weight around 70 kDa. The results of cluster analysis based of SDS/PAGE under reduction conditions indicated that genetic diversity between Turkish, Syrian, and Iranian and Australian accessions is pronounced, and Turkish accessions are closer to both Syrian and Iranian accessions than the relation between Syrian and Iranian. This suggested that crosses between the Iranian and Syrian accessions could create more genetic variability than crosses with Turkish accessions. The distribution of Turkish and Syrian accessions between more than one clusters revealed that genetic diversity and geographic distribution were independent of each other. PCA showed that all accessions were separated on the first principal component, indicating that the accessions showed a good association, due, probably, to parallel evolution.
Eighteen L. inconspicuous accessions collected from different countries were evaluated for variations of seed weight, seed protein content, and electrophoretic patterns of the total seed proteins analyzed under reducing conditions. They exhibited a reasonable genetic variability for the evaluated traits. This genetic variability revealed that improvement through simple selection for these traits is possible. The variation between the seed size of this accessions was attributed to the development process or the life cycle of the plant, and the environmental condition to which the mother plant is exposed. On the other hand, the variation in protein content among the different accessions may be due to genotype and/or seasonal influences. The relationship between protein content and 100 seeds weight in the evaluated accessions was reversible, the accession showed the lowest quantity of the total seed proteins was the accession that exhibited highest weight of 100 seeds and nearly vice versa. Each accession gave a different electrophoretic pattern except the two accessions collected from Iran, exhibited an identical one. The difference in 100 seed weight and total protein content of these accessions indicated that they are not genetically identical. The variation in the electrophoregram of the evaluated accessions located in the bands with molecular weight more than 98 kDa, the heavy subunits of alpha-lathyrin subunits and the region molecular weight around 70 kDa. The results of cluster analysis based of SDS/PAGE under reduction conditions indicated that genetic diversity between Turkish, Syrian, and Iranian and Australian accessions is pronounced, and Turkish accessions are closer to both Syrian and Iranian accessions than the relation between Syrian and Iranian. This suggested that crosses between the Iranian and Syrian accessions could create more genetic variability than crosses with Turkish accessions. The distribution of Turkish and Syrian accessions between more than one clusters revealed that genetic diversity and geographic distribution were independent of each other. PCA showed that all accessions were separated on the first principal component, indicating that the accessions showed a good association, due, probably, to parallel evolution.
Genetic Diversity Study in the Genus Lathyrus Using RAPD Analysis
Molecular markers based on PCR analysis using arbitrary 10-mer primers (RAPD) was used to identify and assess the genetic diversity of accessions of seven Lathyrus species: L. aphaca, L. clymenum, L. ochrus, L. annuus, L. cicera and L. sativa tingitanus. A total of 253 amplified products ranging in size from 300 bp to 3100 bp were generated by PCR amplification using 18 primers. Genetic similarities were estimated on the basis of pair-wise comparisons of RAPD marker analysis and dendrogram of genetic relationship between species were constructed. The RAPD data obtained were sufficient to distinguish between accessions and to separate these accessions by clustering them according to species. A high degree of genetic variability was recorded between studied species.
Genetic Variability in Lathyrus spp. as Revealed by Karyotype Analysis
CYTOLOGIA, 2009
The genetic variability based on karyotype formula was studied for 40 accessions of genus Lathyrus. All accessions have 2nϭ14 chromosomes. Karyotype formula has a great uniformity among accessions of each species, suggesting interaspecific stability in Lathyrus species. Our data allowed the differentiation of several accessions among sections Lathyrus, Linearicarpus and Clymenum in number of m chromosome. The variation among section Lathyrus supports that section Lathyrus is not fully constant as has been postulated. Satellites were detected in a pair of chromosome in L. sativus (TUN and AFG). On the otherhand, B-chromosome was detected in L. sativus (USSR, BAN, CAN, and PAK) L. gorgoni (JOR) and L. annus (SYR). This finding may be due to the variation in DNA amount by increase in the non-repetitive sequence. The variation in size of chromosomes of the members of Lathyrus is associated with the evolution in the genus.
2020
Grass pea belongs to the legume family and it is traditionally used as a grain for human consumption while its foliage is used for fodder. In this study morpho-agronomic evaluation and characterisation of different grass pea accessions (Lathyrus sativus L.) from Slovenia and Bosnia and Herzegovina (B&H)/Republic of Srpska was performed. A collection of 8 grass pea accessions was cultivated in Slovenia (Jablje) and Bosnia and Herzegovina/Republic of Srpska (Banja Luka) in the open field trials during the growing season of 2019. A number of quantitative and qualitative IPGRI descriptors for Lathyrus spp. concerning the vegetative growth, stem, branch, leaf, inflorescences, pods, and seeds were measured and/or visually estimated. The results of the present study will add value through the enrichment of the Lathyrus spp. collection in both countries, improving the existing data and documentation, as well as creating a certain basis for further studies of the morpho-agronomic traits of t...
The study of genetic variation for Lolium perenne using ISSR molecular markers
International Journal of Biosciences (IJB), 2014
Genetic variation for 12 accessions of Lolium perenne were surveyed using the number of 12 ISSR primers, that the number of 10 primers can be scored. The ISSR primers can be produced the number of 62 bands, which the polymorphism was showed for the number of 47 bands. The average of bands was 6.2 for each primer. The primer of IS9 showed the highest number of band (11 bands) and IS15 showed the lowest number of band (3 bands). A desirable polymorphism between genotype was observed based on ISSR markers, which the primers of IS9, IS10, IS13 and IS16 were determined for genetic variation study in Lolium perenne as desirable primers. Average PIC in the used primers was 0.33 that the highest amount of PIC related to primer IS12 that amount of PIC was 0.45 in this primers and primers IS3 and IS14 with the lowest value of PIC don't have ability in the separation of accessions. Yatsyn (G11) had the most genetic distance with Grenisle (G7). Cluster analysis and Scatter plot based on first and second axis from principal coordinate analysis for genotypes, showed that genetic variation did not agreement with the geographical distribution. Therefore the genotypes which had the most genetic distance based on used ISSR marker can be considered as suitable material in breeding programs for using hetrosis.
Genetic variation among diff erent accessions of Lathyrus sativus (Fabaceae) revealed by RAPDs
2012
Th e relationship between genetic similarity and larger geographical distance among populations of plant species has been attributed to a number of diff erent factors mostly breeding systems. We assessed this relationship among fi ve accessions of selfi ng legume Lathyrus sativus L. (grass peas, Fabaceae) using RAPDs by including 10 random ly selected individuals from each accession. Five primers produced 73 clear, reproducible and scorable polymorphic bands. Th e percentage polymorphic bands ranged from 20.6% in German to 60.3% in Polish accessions. Th e range of Nei's within-accession genetic diversity was wide, ranging from 0.075 in German to 0.25 in Polish accessions. Partitioning of total genetic diversity by AMOVA indicated 76.44% genetic diversity among accessions and 23.56% within accessions, indicating that L. sativus is a selfi ng species. Th e shortest genetic distance was detected between German and Iranian accessions (0.202), while the greatest genetic distance was ...
Karyotype study in some Lathyrus L. accessions of Iran
Twenty accessions of cultivated grass pea (Lathyrus sativus L.) and wild species of Lathyrus L. collected from western and southern regions of Iran were evaluated for their mitotic metaphase chromosomal characteristics. All populations comprising 16 populations of cultivated L. sativus and 4 wild populations of Lathyrus L. were diploid, 2n=2x=14 chromosomes. There were significant variations among populations in the number of metacentric, submetacentric and subtelocentric chromosomes, the longest and shortest chromosome lengths, total haploid complement, arm ratios and centromeric index. The karyotypic formula of wild Lathyrus spp. populations were quite varied from those in native grass pea cultivars in having either two or three pairs of subtelocentric chromosomes and accordingly containing one or two metacentric chromosomes. In addition, total chromosome length (TCL) in wild Lathyrus L. accessions was less than those for grass pea populations. The mean length of the largest chromosome differed significantly among populations, with populations 5 having the largest chromosome (7.2µm). The mean length of the shortest chromosome did not differ among populations, with wild populations from Ilam (populations 17, 18 and 19) having the lowest value. The arm ratios and centromeric index (CI) data revealed adverse trends, whilst wild populations of Lathyrus L have the highest arm ratios and the lowest CI values. There were only slight differences in the mean of arm ratios among the native grass pea populations considering a range of 1.3 to 1.9 for this morphological trait.
Genetic variations in accessions of Lathyrus sativus
2007
Eighteen grass pea (Lathyrus sativus L.) accessions (donated from USDA germplasm) collected from different geographical regions were evaluated for variations of seed weight, and seed protein content. Environmental factors may not be most appropriate for explaining variations in seed weight. The presence of small-seeded accessions in Eastern Africa possibly indicate this sub-region as a new center of origin of L. sativus. There were no correlations between protein content and seed weight indicating genetic independence. Multivariate analysis (cluster and factor analysis) based on protein analysis data showed a high genetic variability among the accessions of different geographical regions and a low variability among the accessions of the same region.