Rapid expression of functional genomic libraries (original) (raw)

PCR-linked in vitro expression: a novel system for high-throughput construction and screening of protein libraries

Suang Rungpragayphan

FEBS Letters, 2003

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Rapid in vitro protein synthesis pipeline: a promising tool for cost-effective protein array design

Satheesh Natarajan

Molecular BioSystems, 2014

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An Alternative Platform for Protein Expression Using an Innate Whole Expression Module from Metagenomic DNA

Ho-Dong Lim

Microorganisms, 2019

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CCD 2: design constructs for protein expression, the easy way

George Damaskos

Acta Crystallographica Section D Structural Biology

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Protein expression from synthetic genes: Selection of clones using GFP

Pascal Demange

Journal of Biotechnology, 2007

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Gene design, cloning and protein-expression methods for high-value targets at the Seattle Structural Genomics Center for Infectious Disease

Don Lorimer

Acta Crystallographica Section F Structural Biology and Crystallization Communications, 2011

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Faculty of 1000 evaluation for A cell-free protein synthesis system for high-throughput proteomics

Sheila McCormick

F1000 - Post-publication peer review of the biomedical literature

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A universal approach to gene expression engineering

Jenny Nesje

Synthetic Biology

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A universal method for gene expression engineering

Joern Kalinowski

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High-throughput protein expression of cDNA products as a tool in functional genomics

Susanne Gräslund

Journal of Biotechnology, 2000

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The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning

Nick S Berrow

Methods in molecular biology (Clifton, N.J.), 2009

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TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression

Θανάσης Νιάρχος

PLOS ONE, 2017

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Multi-Host Expression System for Recombinant Production of Challenging Proteins

Joop van den Heuvel

PLoS ONE, 2013

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A software tool to accelerate design of protein constructs for recombinant expression

Per Kraulis

Protein Expression and Purification, 2010

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A quick in vitro pathway from prokaryotic genomic libraries to enzyme discovery

Gilles Ravot, Cécile Persillon

BioTechniques, 2008

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A versatile ligation-independent cloning method suitable for high-throughput expression screening applications

Nick S Berrow

Nucleic Acids Research, 2007

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Chromosome-based Strategies for the Generation of Novel and Efficient Mammalian Expression Systems

Juergen Bode

2005

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A complete approach for recombinant protein expression training: From gene cloning to assessment of protein functionality

Ana Carolina Migliorini Figueira

Biochemistry and Molecular Biology Education, 2005

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Protein microarray generation by in situ protein expression from template DNA

Juergen Burger

Engineering in Life Sciences, 2014

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Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression

Erqing JIN

Synthetic and systems biotechnology, 2017

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Episomal Vectors for Rapid Expression and Purification of Proteins in Mammalian Cells

Giovanni Magistrelli

Protein Purification, 2012

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A streamlined implementation of the glutamine synthetase-based protein expression system

veronica chang

BMC Biotechnology, 2013

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An automated small-scale protein expression and purification screening provides beneficial information for protein production

Bruno Martinez

Journal of Structural and Functional Genomics, 2004

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Recombinant protein expression for therapeutic applications

Dana Andersen

Current Opinion in Biotechnology, 2002

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A high-throughput expression screening platform to optimize the production of antimicrobial peptides

Denise Salzig

Microbial Cell Factories

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Do-it-yourself: construction of a custom cDNA macroarray platform with high sensitivity and linear range

Rafael Van Den Bergh

BMC Biotechnology, 2011

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HuCAL PLATINUM, a Synthetic Fab Library Optimized for Sequence Diversity and Superior Performance in Mammalian Expression Systems

stefanie thiel

Journal of Molecular Biology, 2011

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Quikgene: A gene synthesis method integrated with ligation-free cloning

Kun Mei

Analytical Biochemistry, 2011

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How to approach heterogeneous protein expression for biotechnological use: An overview

Daniela Jamrichova

Nova Biotechnologica et Chimica, 2017

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Integrating Gene Synthesis and Microfluidic Protein Analysis for Rapid Protein Engineering

Ekaterina Petrova

2015

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Available methods for assembling expression cassettes for synthetic biology

Xingyuan Ma

Applied Microbiology and Biotechnology, 2012

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Predictive mutagenesis of ligation-independent cloning (LIC) vectors for protein expression and site-specific chemical conjugation

Knud j. Jensen

Analytical Biochemistry, 2011

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Construction of a new family of high efficiency expression vectors: Identification of cDNA clones for human Liver proteins

Keith Stanley

The EMBO Journal

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A novel system for stable, high-level expression from the T7 promoter

Diana Mikiewicz

Microbial Cell Factories, 2012

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