Total capillary bed in striated muscle of guinea pigs native to the Peruvian mountains (original) (raw)
Related papers
Microvascular …, 1984
The stereomorphological arrangement of skeletal muscle capillaries depends on many factors. Genetic biochemical composition of muscle fibers, age, and training can interfere with the growth of the capillary network. This study is focused on the age effect of chronic reduction of blood flow, as in arterial stenosis, in skeletal muscle network of young rats when compared with adult rats. It has been observed that there is a statistically significant decrease of the length of short capillaries which cross the muscle fibers. In adult rats the opposite occurs.
Capillary Network Morphometry of Pig Soleus Muscle Significantly Changes in 24 Hours After Death
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2017
Capillary network characteristics are invaluable for diagnostics of muscle diseases. Biopsy material is limited in size and mostly not accessible for intensive research. Therefore, especially in human tissue, studies are performed on autopsy material. To approach the problem whether it is reliable to deduce hypotheses from autopsy material to explain physiological and pathological processes, we studied capillarity in pig soleus muscle 1 and 24 hr after death. Capillaries and muscle fibers were immunofluorescently marked, and images were acquired with a confocal microscope. Characteristics of the capillary network were estimated by image analysis methods using several plugins of the Ellipse program. Twenty-four hours after death, the measured characteristics of the capillary network differ by up to 50% when compared with samples excised 1 hr after death. Muscle fiber diameter, the measured capillary length, and tortuosity were reduced, and capillary network became more anisotropic. T...
Perfused capillary surface area in postural and locomotor skeletal muscle
Microvascular Research, 1982
A measure was made of the perfused capillary surface area per gram of tissue (S,) in postural, anterior latissimus dorsi (ALD), and locomotor, posterior latissimus dorsi (PLD), skeletal muscles in chickens. The animals were anesthetized with L.A. Thesia and the ALD and PLD muscles were prepared for observation using a modification of the method developed by R. E. Klabunde and P. C. Johnson (1977, Amer. J. Physiol. 232, H41 I-417). S, was determined from the relationship S r = TdLO,, where d = capillary diameter, L = capillary length, and Or = number of perfused capillaries per gram. Capillary lengths and diameters were measured directly through the microscope. Estimates of Or were measured in two ways: by a flow method (OF), and by an histochemical method (OF). It was observed that the average capillary diameter in PLD was the same as in ALD muscles, but the average capillary length in PLD was two times longer than in ALD muscles. Both the flow and histochemical methods yielded values of Or that gave similar values of S, for PLD muscles (SF = 24.7 + 20.0 cm'ig, S," = 13.7 * 14.4 cm'ig), but different values for ALD muscles (Sp = 9.8 f 6.8 cm'lg, S p = 42 8 ? 19.1 cm'lg). The difference between SF and S," for ALD muscles could be explained by the more irregular and longer perfusion path observed in postural versus locomotor muscle. The flow method appears to underestimate S, in capillary beds with irregular perfusion paths. The results indicate that only a small fraction of the total capillary bed was perfused in resting skeletal muscle and that S," for ALD was approximately three times larger than SF for PLD.
Microvascular Research, 1992
Except in thin muscles, the analysis of muscle capillarity depends on direct ultrastructural visualization or histochemical identification of the capillaries using stains for basement membrane or reactions for endothelial cell enzymes. The accuracy of existing histochemical methods for detecting capillaries has not been systematically tested, however. The purpose of the present study was to compare muscle capillarity determined by two methods currently in use, Griffonia simplicifolia I lectin (GSI) and ATPase, with two alternative capillary markers, Lycopersicon esculentum lectin (LEA) and MRC OX.43 antigen, Capillarity, expressed as capillaries around fibers (CAF), was determined in the soleus, extensor digitorum longus, and sternomastoid muscles from 4-month-old female rats. The GSI, LEA, and MRC OX.43 capillary markers gave identical results for each muscle, confirming their validity as cytochemical probes. In contrast, the capillary ATPase method gave significantly lower CAF determinations, with a differential effect of preincubations at pH 4.4 vs 4.0. These data suggest that capillary ATPase acid stability varies within the capillary bed. The use of one or more of the alternative capillary markers tested is suggested for studies of muscle capillarity to confirm that binding sites or enzyme activity used for a given probe is expressed under both control and experimental conditions. o tsar Academic RUSS, IK 112 002&2S62/92 55.00
Morphometrical and Histochemical Study of the Cross Striated Skeletal Muscles of Pigs
In this study morphological and histochemical parameters of the striated skeletal muscle of pigs of the Slovak large White breed was analyzed. New-born, one-day-old (1-day), three-day-old (3-day), eighteen-day-old (18-day), forty-eight-day-old (48-day), eighty-four-day-old (84-day), one-hundred-twenty-day-old (120-day), one-hundred-ninety-two-day-old (192-day) and finally one-thousand-fifty-five-day-old pigs (1055-day) were used for this purpose. Samples from the three muscles m. triceps brachii (MTB), m. longissimus dorsi et lumborum (MLD) and m. rectus femoris (MRF) were. The samples were collected by necropsy, fixed in liquid nitrogen and sliced on a freezing microtome. All the samples were stained with hematoxylin-eosin, toluidine blue, oil red “0”, and succinate dehydrogenase (SDH) was used for differentiation of individual types of muscle fibres. Myogenesis is completed in the third day of the post-natal life of pigs. The creation of new muscle fibres is limited by the number ...
A histochemical ATPase method for the demonstration of the muscle capillary network
Journal of Histochemistry & Cytochemistry, 1993
A histochemical method for demonstration of the cap== in skeletal muscle of birds is proposed. The present method, which is a modification of a previously reported myosin ATPase technique used for simultaneous staining of capillaries and fiber types, provides an accurate count of capillaries associated with different fiber types in avian skeletal musdes. We have applied the original and the modified method to serial adjacent sections of certain skeletal musdes and our results show that after the application of the original technique: (a) in muscles having dark Type Ii fibers, these fibers produce a masking effect on their adjacent capillaries: (b) a consistent and significant undetcounting in cap-' Supported in part by the ComisiBn Asesora Interministerial en Ciencia Tecnologia, Grant PB90-0017.
Capillary Network in Slow and Fast Muscles and in Oxidative and Glycolytic Muscle Fibres
Image Analysis & Stereology, 2011
The aim of this study was to compare capillary network in slow and fast muscles and also in oxidative and glycolytic muscle fibres. Soleus (SOL) and extensor digitorum longus (EDL) muscles were excised from five female rats. Capillaries and muscle fibres were demonstrated on thick tissue sections by a triple immunofluorescent method. Stacks of perfectly registered optical images were captured by a confocal microscope and further analysed. Applying stereological methods (POINTGRID, FAKIR and SLICER plugin- modules of the Ellipse programme), we estimated the mean length of capillaries, adjacent to individual muscle fibre, per unit fibre length (Lcap/Lfib), per unit surface area of the fibre (Lcap/Sfib) and per unit fibre volume (Lcap/Vfib) in the slow SOL and in predominantly fast EDL muscle, and separately in oxidative and glycolytic fibres of EDL muscle. The length of capillaries per unit fibre length was larger in SOL than in EDL muscle, however, capillary length per unit fibre vol...
Sequential perfusion of skeletal muscle capillaries
Microvascular Research, 1985
Rats were injected intraarterially with a fluorescent dye that binds to capillary endothelium, thereby labeling any capillary through which it has passed. After 10, 15, or 30 set of circulation of the dye blood flow was interrupted, the gastrocnemius was frozen, and the density and distribution of labeled capillaries were measured in transverse sections of the central portion of the medial head. These tissue sections were then counterstained by the myosin ATPase method for capillaries to mark all capillaries. After 10 set, 45% of all capillaries were labeled and after 15 set, 59% of all capillaries were labeled. Thirty seconds after injection, all capillaries were labeled with the fluorescent dye. In all three time intervals, the distributions of labeled capillaries were ordered, suggesting that there is a tissue-level control mechanism for regulating capillary perfusion to maintain relatively short maximal oxygen diffusion distances. 0 1985 Academic PWSS. hc.