Pseudomonas baetica sp. nov., a fish pathogen isolated from wedge sole, Dicologlossa cuneata (Moreau) (original) (raw)
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Phenotypic and Genotypic Comparison of Pseudomonas stutzeri in Freshwater Fish in Indonesia
Journal of Agricultural Science and Technology B, 2015
Pseudomonas stutzeri caused an outbreak of freshwater fish in Luwuk Banggai (tilapia and catfish), Bali (tilapia), Jambi (tilapia and catfish) and Tanjung Pinang (catfish). The study was purposed to comprehensively identify special phenotypic and genotypic characteristics of P. stutzeri isolated from several areas in Indonesia, including its morphometric and biochemical characteristics and molecular variation. Bacteria were isolated from internal organs (kidney, ulcer and eye) of fish. They were then identified using morphology and biochemical test. DNA isolates were entirely extracted, amplified and reversed on 16S rRNA region, and further then were sequenced. Phylogenetic trees of bacteria were constructed using neighbor-joining and maximum-parsimony methods. The colony were similar, such as rod shape (Jambi, Tanjung Pinang, Bali), bacil shape (Luwuk Banggai), transparant in tryptic soy agar (TSA) (Luwuk Banggai), creamy beige in glutamate starch phenol red (GSP) (Bali), gram negative, motile, no reaction in the oxidative-fermentative test, positive result in catalase and oxidase test, negative in lysine decarboxylase and ornithine decarboxylase test and positive result in indole test; gelatin was degraded (only Bali), urea was not degraded, no color change in Methyl-red and Voges-proskaeur (MR-VP) test; acid not produce from glucose, inositol or sucrose. Citrate was utilized by some isolates: positive (Jambi, Tanjung Pinang) and negative (Bali, Luwuk Banggai). Results showed us that isolates of Jambi, Bali and Tanjung Pinang were monophyletic species with P. stutzeri S8 and ZH-1 comparing to gen bank. However, merely phenotypic analysis among Pseudomonas sp. was confused compared to each other.
Journal of Fish Diseases, 2007
The biochemical, serological and genetic characteristics of six strains of Pseudomonas anguilliseptica isolated from cod, Gadus morhua, in Scotland were compared to well characterized isolates of this same bacterial species but of different origin. Biochemical and physiological analyses showed that this group of isolates was highly homogeneous, their characteristics matching previous descriptions of the pathogen. Similar results were obtained for the six cod isolates in the serological assays, all of them belonging to the serotype O1. Marked homogeneity was observed also in the genetic study, analysed by means of RAPD, ERIC-PCR and REP-PCR procedures, showing that they were similar to isolates from gilthead seabream, Sparus aurata, black spot seabream, Pagellus bogaraveo, and turbot, Psetta maxima. Virulence assays demonstrated that the cod isolates were highly pathogenic for turbot and sole, Solea senegalensis, with LD50 between 7.6 × 104 and 5 × 107 bacterial cells per fish.
Detection of Virulence factors of Pseudomonas species isolated from fresh water fish by PCR
Benha Veterinary Medical Journal, 2016
This study was conducted on 100 diseased Nile tilapia (O. niloticus) fish of various sizes collected from different fish markets in Kaliobia Governorate to estimate the prevalence of Pseudomonas infection and detection of some virulence genes in the isolated P. aeruginosa strains. The results of bacteriological examination revealed that the prevalence of Pseudomonas septicemia with Pseudomonas species isolation was 17.0% (17 \ 100 examined fish). These cases were attributed to P. anguilliseptica; P. aeruginosa and P. fluorescens (14/43.7%; 12/37.5% and 6/18.8%), respectively. In addition, 32 Pseudomonas species were isolated, 11from liver samples (34.4%); 10 from kidney samples (31.2%); 6 from gill samples (18.8%) and 5 from skin samples (15.6%). Moreover, 14 P. anguilliseptica were isolated with an incidence of 35.7%, 28.6%, 21.4% and 14.3% followed by 12 P. aeruginosa 33.3%, 25%,16.7% and 25% respectively; 6 P. fluorescens 33.3%; 50.0%,16.7% and 0.0% from the liver, kidney, gill and skin samples respectively. The in-vitro antimicrobial sensitivity test showed that the isolated Pseudomonas strains were sensitive to gentamycin; enrofloxacin; norfloxacin; ciprofloxacin and florphenicol. Meanwhile; they were intermediate sensitive for doxycycline; sulfa-trimethoprim; oxytetracycline; nalidixic acid and streptomycin. In contrast, they were resistant for cefotaxime; erythromycin; amoxicillin; methicillin; oxacillin and ampicillin. Moreover, the PCR results revealed that, opr L and exo S virulence genes were detected in all six studied strains (100.0%). Meanwhile, phz M virulence gene was detected in 5 out of 6 studied strains (83.3%) and tox A virulence gene was detected in 4 out of 6 studied strains (66.7%) i.e., all studied strains were Ps. aeruginosa and all of them were virulent strains.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2009
Nine Gram-negative, rod-shaped, non-spore-forming isolates with identical or very similar repetitive-sequence-based PCR profiles were recovered from an evaporative lagoon in Mexico. Two strains, designated 1N T and 3N, had virtually identical 16S rRNA gene sequences and, on the basis of these sequences, were identified as members of the genus Pseudomonas, with Pseudomonas peli R-20805 T as the closest relative. All nine isolates had practically identical whole-cell protein profiles. The major fatty acids [C 16 : 0, C 18 : 1 v7c and summed feature a (C 16 : 1 v7 and/or C 16 : 1 v6c)] of strains 1N T and 3N supported their affiliation with the genus Pseudomonas. The DNA-DNA reassociation values with respect to P. peli LMG 23201 T and other closely related Pseudomonas species were ,15 %. Physiological and biochemical tests allowed phenotypic differentiation of the strains analysed, including strain 1N T , from the five phylogenetically closest Pseudomonas species. On the basis of the data obtained by using this polyphasic taxonomic approach, the nine strains represent a novel species, for which the name Pseudomonas cuatrocienegasensis sp. nov. is proposed. The type strain is 1N T (5LMG 24676 T 5CIP 109853 T ).
International Journal of Systematic Bacteriology, 1989
The deoxyribonucleic acid (DNA):ribosomal ribonucleic acid (rRNA) hybridization technique was used to reveal the relationships and taxonomic positions of an additional 83 strains belonging to 43 saprophytic or pathogenic Pseudomonus species and 29 named and unnamed Pseudomonus-like strains. The DNA:rRNA hybrids were characterized by the following two parameters: (i) the temperature at which one-half of the hybrid was eluted and (ii) the percentage of rRNA binding (the amount of rRNA bound per 100 pg of filter-fixed DNA). We also used, for a limited number of strains, numerical analysis of carbon assimilation tests to delineate the finer taxonomic relationships of organisms. Of the 83 strains examined, 78 could be definitely assigned either to an rRNA branch or to an rRNA superfamily within the Proteobucteriu. Only 25 of our strains belong in the genus Pseudomonus sensu strict0 (our PseudomonusJluorescens rRNA branch). In general, about two-thirds of the named Pseudomonas species have been misclassified and are distributed over at least seven genera all through the Proteobacteriu. These organisms need to be reclassified and generically renamed according to their phylogenetic rglationships. However, more detailed phenotypic and genotypic studies are necessary before definite nomenclatural proposals can be made. A comprehensive list of the phylogenetic affiliations of the Pseudomonus species is included.
PseudoMLSA: a database for multigenic sequence analysis of Pseudomonas species
BMC Microbiology, 2010
Background: The genus Pseudomonas comprises more than 100 species of environmental, clinical, agricultural, and biotechnological interest. Although, the recommended method for discriminating bacterial species is DNA-DNA hybridisation, alternative techniques based on multigenic sequence analysis are becoming a common practice in bacterial species discrimination studies. Since there is not a general criterion for determining which genes are more useful for species resolution; the number of strains and genes analysed is increasing continuously. As a result, sequences of different genes are dispersed throughout several databases. This sequence information needs to be collected in a common database, in order to be useful for future identification-based projects.
International journal of systematic bacteriology, 1996
We compared the 16S rRNA gene sequences of 14 strains of Pseudomonas stutzeri, including type strain CCUG 11256 and strain ZoBell (= ATCC 14405), which represented the seven P. stutzeri genomovars (DNA-DNA similarity groups) that have been described. Our sequence analysis revealed clusters which were highly correlated with genomovar clusters derived from DNA-DNA hybridization data. In addition, we identified signature nucleotide positions for each genomovar. We found that the 16S rRNA gene sequences of genomovar 6 strains SP1402T (T = type strain) and LS401 were different enough from the sequence of the type strain of P. stutzeri that these organisms should be placed in a new species, Pseudomonas balearica. The type strain of P. balearica is strain SP1402 (= DSM 6083).
Journal of Applied Microbiology, 2005
Aims: Three broadly used typing methods were employed in order to assess and compare the identification and classification of environmental Pseudomonas strains. The reproducibility, typeability and discriminatory power of the methods were also compared to evaluate their application. Finally, the potential impact on public health of the isolates is to be discussed. Methods and Results: Pseudomonas strains (160) isolated from the aquatic environment in Greece and identified by a rapid identification commercially available system (API20NE), were subjected to whole-cell protein electrophoresis (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and Randomly Amplified Polymorphic DNAs (RAPD) using two 10-mer primers. In general, the obtained results were in agreement. Twenty isolates that could not be identified by the API20NE system were classified by the other methods. Conclusions: Rapid identification systems may serve only for a first rough identification of environmental Pseudomonads. In order to acquire further information, so that conclusions about their role in the ecosystem and human health could be drawn, other phenotypic or genotypic methods have to be applied. Significance and Impact of Study: It is important, from a public health point of view, to monitor the identities of environmental Pseudomonas isolates using specific methods due to their ubiquity, heterogeneity and their pathogenicity, either established or potential.
Acta Veterinaria, 2016
For the purpose of precise antibiotic susceptibility testing it is necessary to clearly distinguishThe results of the standard PCR test, 16S rRNA gene sequencing and MALDI-TOF analysis confirmed 35 strains to belong to theThree strains were positive in both standard PCR tests forThree strains were negative in both standard PCR tests forAlthough modern test methods that have very high specificity (PCR, 16S rRNA gene sequencing, MALDI TOF) were used in this study, precise differentiation between
Systematic and Applied Microbiology, 1996
As a consolidated effort on the part of several laboratories, partial and nearly complete sequence determinations of 16S rRNA genes have been applied as one of several analytical methods in a polyphasic study of the pseudomonads. Nearly-complete sequences have been determined of the PCR-amplified 16S rRNA genes of 21 species of the genus Pseudomonas (sensu stricto), including multiple strains of most species. Phylogenetic branching orders and the natural intrageneric relationships among the species have been inferred through sequence comparisons and cluster analysis and have not shown any obvious recognizable correlation with results derived through standard phenotypic criteria commonly used to group the species. This paper also focuses on the ability of 16S rRNA gene sequences, particularly the hypervariable sequence regions, to be used as nested identification markers and as target sites for the development of 16S rRNA sequence-based strategies for the identification of species of the genus Pseudomonas.