Carbohydrate antigen profiles on human erythroleukemia cell lines HEL and K562 (original) (raw)

The expression of major carbohydrate antigens carried by polylactosaminyl chains in human erythroleukemia cell lines, K562 and HEL, was investigated by applying monoclonal antibodies recognizing specific carbohydrate determinants. The two cell lines showed common differences in their glycolipid compositions: (1) the presence of signifucant amounts of ganglio-series glycolipids. which are absent in normal erythrocytes; and (2) a remarkable reduction in the amount of globo-series glycolipids. which are the major glycolipids in normal human erythrocytes. A variety of differences were also detected in the carbohydrate antigens carried by lacto-series glycolipids and glycopro-R EMARKABLE ALTERATION of cell surface carbohydrates associated with malignant transformation has been noted for many years."2 Recently, From Biochemical Oncology.