Synthesis, characterization, and evaluation of antibacterial activity of transition metal oxyde nanoparticles (original) (raw)
Related papers
Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2011
Inorganic metal oxides may serve as effective disinfectants, due to their relatively non-toxic profile, chemical stability and efficient antibacterial activity. Among metal oxide nanoparticles, zinc oxide demonstrates significant bacterial growth inhibition on a broad spectrum of bacteria, mainly by catalysis of reactive oxygen species (ROS) formation from water and oxygen. Aqueous suspensions of ZnO nanoparticles (ZnO nanofluids) are the preferred formulation for using the antibacterial agent in liquid phases and for the incorporation of the nanoparticles in different commercial products. However, ZnO nanoparticles in aqueous media tend to aggregate into large flocculates, due to their hydrophobic nature, and thus do not interact with microorganisms effectively. In this study, zinc oxide was combined with iron oxide to produce magnetic composite nanoparticles with improved colloidal aqueous stability, together with adequate antibacterial activity. For this purpose, the Zn/Fe oxide composite nanoparticles were synthesized by basic hydrolysis of Fe 2+ and Zn 2+ ions in aqueous continuous phase containing gelatin. The obtained composite nanoparticles were composed of iron oxide, zinc oxide and zinc ferrite phases. The effect of the weight ratio [Zn]/[Fe] of the composite nanoparticles on their properties (composition, size, magnetic behavior and colloidal stability) was elucidated. The antibacterial activity of these nanoparticles was tested against Staphylococcus aureus and Escherichia coli and was found to be dependent on the weight ratio [Zn]/[Fe], i.e., the higher the ratio, the higher the antibacterial activity. In addition, the activity against Staphylococcus aureus was significantly higher than that observed against Escherichia coli.
Asian Journal of Pharmaceutical and Clinical Research, 2020
Objective: This study deals with the synthesis of iron oxide nanoparticles by sol-gel technique, their characterization and antibacterial activity of these nanoparticles against Staphylococcus epidermidis. Methods: Hematite (α-Fe 2 O 3) nanoparticles were successfully synthesized by sol-gel method using tetraethyl orthosilicate as a precursor. The structural morphology, size, and chemical state of synthesized iron oxide nanoparticles have been investigated by X-ray diffractometer (XRD), transmission electron microscopy, Fourier transform infrared spectroscopy, and ultraviolet-visible spectroscopy. The antibacterial activities of these iron oxide nanoparticles were investigated on a pathogenic bacteria S. epidermidis, by measuring the zone of inhibition and colony-forming units on solid medium and by measuring the optical density of the culture solution. Antibacterial activity of iron oxide nanoparticles was also compared with well-known standard antibiotics. Results: It was confirmed from XRD data that the synthesized iron oxide nanoparticles were hematite (α-Fe 2 O 3) nanoparticles. Average particle size of the Fe 2 O 3 nanoparticles was found to be 38.57 nm by XRD characterization. The antibacterial activity of Fe 2 O 3 nanoparticles was almost comparable to the most of the standard antibiotics (taken for comparison), but Fe 2 O 3 nanoparticles were found to be more effective than antibiotic ampicillin and sulfatriad toward S. epidermidis. Conclusion: This study shows that Fe 2 O 3 nanoparticles possess good antibacterial properties; therefore, these metal nanoparticles may be used in place of antibiotics. These inorganic metal nanoparticles can be used by pharmaceutical industries for further research regarding the toxicity study for its use in human being.
Synthesis, Characterization and Antibacterial Activity of Zinc Ferrite Nanopowder
Synthesis of nanosized particles with antibacterial properties are of immense attention in the development of novel pharmaceutical products. Zinc ferrite nanopowder (ZnFe2O4) is known to have excellent bactericidal effects. In this article we present the effectiveness of ZnFe2O4 nanopowder against few bacterial pathogens. ZnFe2O4 nanopowder is prepared by facile solution combustion method from zinc nitrate and ferric nitrate using freshly prepared oxalyl dihydrazide (ODH) as fuel. The structure and morphology of ensuing product was characterized by Powder X-ray Diffraction (PXRD), Fourier Transform Infrared (FTIR) and Scanning Electron Microscopy (SEM) analysis. The PXRD result confirms the formation of spinel cubic structure with Fd 3 m space group matches with JCPDS No. 22-1012. The average particle size calculated by Debye Scherrer formula is 12 nm and the X-ray density of the powder is 2.398 g/cc3. The FTIR spectrum confirms the purity of the sample. The SEM micrograph shows that the morphology of the sample as agglomerated and flakes type of structure. The antibacterial activity of the synthesized ZnFe2O4 nanopowder was done by Luria-Bertani broth (LB) method. ZnFe2O4 nanopowder was investigated with four different bacterial pathogens. The observed result shows antibacterial activity against E.coli, S. aureus, B. tuberculosis and P. aeruginosa bacterial pathogens. The concepts of fundamental molecular mechanism of antibacterial actions are responsible for these results. For targeted drug delivery system, the magnetic nanoparticles of around 10 nm were preferred. Since the prepared samples have these potentials, the magnetic properties of these samples are under investigation.
Synthesis, characterization and use of iron oxide nano particles for antibacterial activity
In this decade, the use of nano particles (NPs) against bacterial growth is increasing day by day due to remarkable alternative properties compared to molecular antibiotics. Thus, the use of iron oxide nanoparticles (IONPs) has proven one of the most important transition metals oxide-based remedy in nanotechnological advances and biological applications due to enriched bio-compatibility of iron. In this study synthesis of IONPs was carried out via co-precipitation method. The crystallographic morphology of the synthesized particles was studied via X-ray dif-fraction which revealed cubic structure of the particles, whereas, the spinal shaped morphology of the prepared NPs was confirmed from scanning electron microscopy. Likewise, the presence of the major elements in the sample was determined through energy dispersive X-ray analysis characterization. Bactericidal effect of the NPs was assessed at pre-defined concentrations (50 and 100 μg/ml) against Gram +ve bacteria Staphylococcus aureus, Gram −ve bacteria Shigella dysentry and Escherichia coli. Bacterial strains, which demonstrate the potential of NPs. The purpose of this study was assessing the structure of the synthesized NPs for protective effect against harmful bacterial activity. K E Y W O R D S bacteria, co-precipitation, iron oxide, nanoparticles, SEM
Antibacterial action of doped CoFe 2 O 4 nanocrystals on multidrug resistant bacterial strains
The bactericidal effect of pristine and doped cobalt ferrite nanoparticles has been evaluated against multiple drug resistant clinical strains by assessing the number of colony-forming units (CFU). Monophasic polycrystalline ferrites have been prepared by the malate-glycolate sol-gel autocombustion method as confirmed by the X-ray diffraction study. Various changes occurring during the preparative stages have been demonstrated using TG-DTA analysis which is well complemented by the FTIR spectroscopy. The antibacterial studies carried out demonstrate a bactericidal effect of the nanoparticles wherein the number of CFU has been found to decrease with doping. Cellular distortions have been revealed through SEM. Variation in the number of CFU with dopant type has also been reported herein.
The iron oxide nanoparticles have been synthesized in co-precipitation method using aqueous solution of ferric and ferrous ions with sodium salt. The synthesis of iron-oxide nanoparticles were validated by UV-Visible spectroscopy which showed higher peak at 370 nm as valid standard reference. An average size of iron oxide nanoparticle found by diffraction light scattering (DLS) particle size analyser, ranges approximately between 10 nm to 120 nm with mean particle size of 66 nm. The X-ray power diffraction (XRD) analysis revealed the crystallographic structure of magnetic particles. Characterization of the mean particle size and morphology of iron oxide nanoparticles confirmed that the iron oxide nanoparticles are nearly spherical and crystalline in shape. Further the antibacterial effect of iron oxide nanoparticles was evaluated against ten pathogenic bacteria which showed that the nanoparticles have moderate antibacterial activity against both Gram positive and Gram negative pathogenic bacterial strains and retains potential application in pharmaceutical and biomedical industries.
Antimicrobial, electrochemical and photo catalytic activities of Zn doped Fe 3 O 4 nanoparticles
In the present study, we report the synthesis of Fe 3 O 4 and Zn-doped Fe 3 O 4 (Zn/Fe 3 O 4) nanoparticles by a simple co-precipitation method. The morphology, structure and optical properties of the samples are characterized by transmission electron microscopy, X-ray diffraction, UV-visible spectroscopy, Fourier transform infrared spectroscopy, energy dispersive spectroscopy and UV-visible spectroscopy. The antibacterial, electrochemical energy storage and photocatalytic properties of the nanoparticles are studied in detail, and the results are discussed. Antibacterial activity of Fe 3 O 4 and Zn/Fe 3 O 4 nano-particles are analyzed by disc diffusion method on Gram-negative pathogen Salmonella typhi and Gram-positive pathogen Staphylococcus aureus. Zn/Fe 3 O 4 nanoparticles show a higher zone of inhibition because of having a larger specific surface area than the pure Fe 3 O 4 nanoparticles. The electrochemical energy storage performances of the nanoparticles are tested in a symmetric two-electrode configuration, and the measurement demonstrated that Zn doping nearly doubles the energy storage properties of the Fe 3 O 4 nanoparticles. The study of the photocatalytic degradation of methyl blue (MB) dye under UV irradiation in the presence of pure and doped Fe 3 O 4 nanoparticles reveal that both nanoparticles act as ideal catalysts for degradation of MB dye.
Materials Today: Proceedings, 2017
In this study, MnFe 2 O 4 NPs were synthesized through Solution combustion method at a temperature of about 450˚C using Oxalyl Dihydrazide (ODH) as a chemical fuel. The synthesized MnFe 2 O 4 nanomaterial was calcined at a temperature of 700˚C. The structural properties and surface morphology of the synthesized NPs were characterized by Powder X-ray Diffraction technique (PXRD) and Scanning Electron Microscopy (SEM). Further these NPs were subjected to antibacterial studies with different strains of Gram-positive and Gram-negative bacteria by Agar well diffusion method and Microbroth dilution technique. The comparison between the bacterial inhibition potential of the synthesized NPs were investigated by the zone of inhibition obtained around the agar wells and the colour change obtained in the titre plate wells containing the bacterial suspension with respect to different concentrations of the as-formed and calcined MnFe 2 O 4 NPs.