Effect of propolis on healing in experimental colon anastomosis in rats (original) (raw)
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Ultrastructural view of colon anastomosis under propolis effect by transmission electron microscopy
World Journal of Gastroenterology, 2008
To evaluate the effect of propolis administration on the healing of colon anastomosis with light and transmission electron microscopes. METHODS: Forty-eight Wistar-Albino female rats were divided into two groups and had colon resection and anastomosis. In group Ⅰ, rats were fed with standard rat chow pre-and postoperatively. The rats in group Ⅱ were fed with standard rat chow and began receiving oral supplementation of propolis 100 mg/kg per day beginning 7 d before the operation and continued until they were sacrificed. Rats were sacrificed 1, 3, 7 and 14 d after operation, and anastomotic bursting pressures measured. After the resection of anastomotic segments, histopathological examination was performed with light and transmission electron microscopes by two blinded histologists and photographed.
Evaluation of the Effects of Anzer Propolis on Intestinal Incision Wound Healing in Rats
Ankara Üniversitesi Veteriner Fakültesi Dergisi, 2021
This study was investigated the wound healing effect of Anzer origin propolis specimens on rats' experimental intestinal incisions. Thirty-four male Wistar albino rats have been divided into five groups, weighing 250-300 gr. Water solution of Anzer propolis (PW3 [n=6], PW7 [n=6]) or ethanolic solution of Anzer propolis (PE3 [n=6], PE7 [n=6]) were administered. Control groups (K3 [n=5], K7 [n=5]) received no treatment. The surgical procedure (day 0) consisted of a laparotomy under general anesthesia, during which the colon was incised and repaired with sutures. The Anzer propolis specimen diluted with physiologic saline was administered by gavage (100 mg/kg/day) for 3 and 7 days before and after surgery to PW3 and PW7 groups. Ethanol dissolved Anzer propolis was administered by gavage (100 mg/kg/day) for 3 and 7 days before and after surgery to PE3 and PE7. Experimental animals have been humanly euthanized on the 3rd and 7th days for biochemical and histopathological evaluations. Serum total oxidant status, total antioxidant status, interleukin-1, interleukin-6, tumor necrosis factor-alpha, myeloperoxidase, hydroxyproline and nitric oxide, levels were measured by ELISA. The incision line sampled to evaluate inflammatory cells, fibroblastic activity, neovascularization, and collagen level. The study indicates that the ethanolic Anzer propolis solution's oral administration has a beneficial effect on intestinal wound healing for seven days pre-and postoperatively (PE7).
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2018
Peritoneal adhesion is a major complication of surgery that can lead to serious problems such as bowel obstruction, pain, infertility and even mortality. Propolis is a honey bee product with anti-inflammatory and anti-oxidant activities that could potentially protect against adhesive surgical complications. Forty 8-weeks-old rats (275 ± 25 g) were divided into five groups: normal group without any surgical procedure, and experimental groups treated with normal saline, 50 mg/kg, 100 mg/kg and 200 mg/kg of propolis. Peritoneal adhesions were examined macroscopically and also, the levels of inflammatory factors (IL-6, IL-1β and TNF-α), growth factors (TGF-β1 and VEGF) were evaluated in the study groups using ELISA. Biochemical indices of oxidative status including Nitric Oxide (NO), Malondialdehyde (MDA) and Glutathione (GSH) were also measured. Peritoneal adhesion scores, IL-1β, IL-6, TNF-α, TGF-β1, VEGF, NO, GSH and MDA levels were significantly different between the study groups (p ...
Propolis and Its Potential to Treat Gastrointestinal Disorders
Evidence-based complementary and alternative medicine : eCAM, 2018
There are a number of disorders that affect the gastrointestinal tract. Such disorders have become a global emerging disease with a high incidence and prevalence rates worldwide. Inflammatory and ulcerative processes of the stomach or intestines, such as gastritis, ulcers, colitis, and mucositis, afflict a significant proportion of people throughout the world. The role of herbal-derived medicines has been extensively explored in order to develop new effective and safe strategies to improve the available gastrointestinal therapies that are currently used in the clinical practice. Studies on the efficacy of propolis (a unique resinous aromatic substance produced by honeybees from different types of species of plants) are promising and propolis has been effective in the treatment of several pathological conditions. This review, therefore, summarizes and critiques the contents of some relevant published scientific papers (including those related to clinical trials) in order to demonstra...
Preliminary Study on Wound Healing Activity of Propolis in Albino Rats
International Journal of Innovative Research in Medical Science, 2016
Background: Infection can lead to delayed wound healing. Recently it has been shown that propolis which is used in complementary medicine has an antibacterial and anti-inflammatory effect. The aim of this study is to determine whether propolis may contribute to wound healing. Material and Methods: Twenty-one male Wistar albino rats were randomly divided into three groups. Group1 and Group 2 were topically treated with propolis ointment and Thiocillin® oinment, respectively while Group 3 was the control group. On incision wound model, Thiocillin® and propolis ointments were applied on wound sites once daily for 30 days and the mean epidermal thickness (MET) at the 30th day was compared while antimicrobial activity of propolis was studied against different pathogens as well. Results: Propolis exhibited in vitro antibacterial activity against Staphylococcus aureus, Escherichia coli, Streptococcus sp. and Pseudomonas sp. It is observed that the MET in the groups of Propolis ointment and...
The Relationship between Gastroprotective Effect of Propolis and Its Antioxidant activity
This study was aimed to investigate the antiulcer effects of propolis and to determine its relationship with antioxidant activity. The antiulcer activities of 70 and 210 mg/kg propolis have been investigated on stress and indomethacin-induced ulcer models in rats. A dose dependant gastroprotective effect of the extract against stress and indomethacin-induced lesions was observed. They reduced incidence of ulceration, significantly reduced mean ulcer score, reduced ulcer index and greatly improved preventive index in both ulcer models but the best result was obtained when propolis used in a dose of 210 mg/kg b.wt .They reversed the decrease in the catalase (CAT) and superoxide dismutase (SOD) level in the stomach tissue of control rats. Additionally, both doses of propolis decreased malondialdehyde (MDA) levels significantly compared to the control. In conclusion, the antioxidant activity of propolis play an important role in its gastroprotective effect mechanism.
Gastroprotective Effect of Propolis Against Male White Mice Gastric Ulcers Induced by Aspirin
Advances in Health Sciences Research
A peptic ulcer is an inflammation of the stomach wall that can cause wounds in the gastric mucosa. Consumption of aspirin over therapeutic doses can cause injury to the stomach. Propolis is known to have an effect to protect stomach damage. This study aims to find out the effect of propolis on male white mice-induced peptic ulcers. A total of 20 mice were divided into 4 experimental groups. The first group was negative control, the second group was positive control (aspirin 350 mg/kg bodyweight for 5 days), the third group was given propolis 500 mg/kg bodyweight for 7 days, and the last group was given propolis then induced with aspirin. The preparations were administered orally. After the treatment, mice were sacrificed to obtain gastric tissue. The tissue was observed macroscopically and microscopically. The data were analyzed descriptively using the average of gastric ulcer index and histopathological analysis. Based on the results of the study, the administration of propolis followed by aspirin has a gastroprotective effect with an average ulcer index of 1, compared to the aspirin group with an ulcer index of 2.5. It can be concluded that the administration of propolis at a dose of 500 mg/kg body weight has a protective effect against male white mice gastric ulcers induced by aspirin.
Effect of Propolis on Experimental Cutaneous Wound Healing in Dogs
This study evaluates clinically the effect of propolis paste on healing of cutaneous wound in dogs. Under general anesthesia and complete aseptic conditions, two full thickness skin wounds (3 cm diameter) were created in each side of the chest in five dogs, one dorsal and one ventral, with 10 cm between them.These wounds were randomly allocated into two groups, control group (10 wounds) and propolis group (10 wounds). Both groups were represented in each dog.The wounds were cleaned with normal saline solution and dressed withmacrogol ointment in control group and propolis paste in propolis group, twice daily till complete wound healing. Measurement of the wound area (cm2) was monitored planimetrically at 0, 7, 14, 21, 28, and 35 days after injury. The data were analyzed statistically. The results revealed a significant reduction in the wound surface area in the propolis group after 14 and 21 days compared to control group.The wound reepithelization, contraction, and total wound healing were faster in propolis group than in control group during five weeks of study. In conclusion, propolis paste has a positive impact on cutaneous wound healing and it may be suggested for treating various types of wounds in animals.
The effectiveness of ethanolic extract of propolis on wound healing in albino rats
Annals of Tropical Medicine and Public Health, 2020
Background: Wound healing is a complex process. So, the use of natural products such as propolis, which has anti-inflammatory and antioxidant properties to accelerate wound healing, was an important target. Aim of work: The aim of this study was to assess the possible wound healing effect of ethanolic extract of propolis (EEP) on full-thickness wound model in albino rats. Materials and Methods: Healthy adult 72 albino rats were used in this study. Excision wound with 1.5 cm length was done on the posterior of the neck. Rats were randomly divided into equal four groups (negative control, positive control, ethanolic extract of propolis (EEP) ointment and tetracycline ointment 1%). Each group was divided into three subgroups that received treatment for 4, 7 and 14 days. Wound area in the experimental group was covered twice daily with a fixed amount of EEP ointment and tetracycline ointment (1%), the negative control group did not receive any treatment, while positive control group treated with physiological saline solution (0.9 % Na Cl). Histological analysis was performed and counting fibroblast, neutrophils, macrophages and new blood vessels in the wound bed was done followed by statistical analysis. Results: The findings show that healing process of excision wound in albino rats treated with the EEP ointment started earlier and had a faster course than the standard tetracycline therapy. Conclusion: The powerful effect of EEP ointment on accelerating wound healing was revealed. This could be an effective strategy for managing delayed wound healing.
Evidence-Based Complementary and Alternative Medicine, 2013
This study focused on the therapeutic effect of a propolis SLNC 106 PI extract on experimental colitis. Wistar adult rats received 0.8 mL rectal dose of one of the following solutions: saline (group S), 20 mg TNBS in 50% ethanol (group TNBS), 20 mg TNBS in 50% ethanol and propolis extract in saline (group TNBS-P), propolis extract in saline (group SP), and 20 mg TNBS in 50% ethanol and 50 mg/kg mesalazine (group TNBS-M). The animals were euthanized 7 or 14 days after the colitis induction. Samples of the distal colon were harvested for the analysis of myeloperoxidase (MPO) enzyme activity and for morphometric analysis in paraffinembedded histological sections with hematoxylin-eosin or histochemical staining. The animals treated with TNBS exhibited the typical clinical signs of colitis. Increased MPO activity confirmed the presence of inflammation. TNBS induced the development of megacolon, ulceration, transmural inflammatory infiltrate, and thickened bowel walls. Treatment with propolis moderately reduced the inflammatory response, decreased the number of cysts and abscesses, inhibited epithelial proliferation, and increased the number of goblet cells. The anti-inflammatory activity of the propolis SLNC 106 extract was confirmed by the reductions in both the inflammatory infiltrate and the number of cysts and abscesses in the colon mucosa.