The interaction of luciferase, flavin mono-nucleotide and long-chain aldehydes in the light reaction catalyzed by preparations of luminous bacteria (original) (raw)

This study investigates the light-producing reaction in luminous bacteria, specifically focusing on the roles of luciferase, flavin mononucleotide (FMN), and long-chain aldehydes. Key experiments were conducted with dark and luminescent strains of Photobacterium to examine the oxidation of FMNH* catalyzed by luciferase in the presence of aldehydes. The methods employed included culturing bacterial strains, enzyme activity measurement, and various assays to delineate the mechanisms involved in the light reaction. Despite understanding the basic components, the exact mechanism and interaction details remain unclear, presenting avenues for future research.