Protein and phospholipid methylation during chemotaxis in Dictyostelium discoideum and its relationship to calcium movements (original) (raw)

Abstract

Suspensions of cyclic AMP sensitive cells of Dictyostelium discoideum responded to a cyclic AMP pulse with increased methylation of a protein of molecular weight about 120,000 and increased phospholipid demethylation. Protein methylation reached its peak 15-30 sec after cyclic AMP addition. Phospholipid demethylation reached its maximum within 2 min and basal levels were recovered in 3 min. S-Adenosyl-L-methionine is probably the methyl donor. In vitro addition of 0.25 mM and 25MuM S-adenosyl-L-methionine to sonicated D. discoideum cells inhibited ATP-dependent 45Ca2+ uptake by 70% and 25%, respectively. Based on these lines of evidence we propose that protein and phospholipid methylation are involved in D. discoideum chemotaxis probably by regulation of intracellular Ca2+ movements.

Loading...

Loading Preview

Sorry, preview is currently unavailable. You can download the paper by clicking the button above.

References (25)

  1. Konijn, T. M., Barkley, D. S., Chang, Y. Y. & Bonner, J. T. (1968) Am. Nat. 102,225-234.
  2. Malchow, D. & Gerisch, G. (1974) Proc. Nat!. Acad. Sci. USA 71, 2423-2427.
  3. Mato, J. M. & Konijn, T. M. (1975) Biochim. Biophys. Acta 385, 173-179.
  4. Henderson, E. J. (1975) J. Biol. Chem. 250,4730-4736.
  5. Green, A. A. & Newell, P. C. (1975) Cell 6, 129-136.
  6. Woollen, D. E. (1972) Alrch. Blochem. Biophys. 150,519-530.
  7. Clarke, M. & Spudich, J. A. (1974) J. Mol. Biol. 86,209-222.
  8. Mockrin, S. C. & Spudich, J. A. (1976) Proc. Natl. Acad. Sci. USA 73,2321-2325.
  9. Mato, J. M., Krens, F. A., van Haastert, P. J. M. & Konijn, T. M. (1977) Proc. Natl. Acad. Sci. USA 74,2348-2351.
  10. Konijn, T. M. & Raper, K. B. (1971) Dev. Biol. 3,725-756.
  11. Gerisch, G. (1962) Wilhelm Roux Arch. Entwicklungsmech. Org. 153,603-620.
  12. Sussman, M. (1966) Proc. Natl. Acad. Sci. USA 55,813-818.
  13. Laemmli, U. K. (1970) Nature (London) 227,680-685.
  14. Hirata, F., Strittmatter, W. J. & Axelrod, J. (1979) Proc. Natl. Acad. Sci. USA 76,368-372.
  15. Lowry, 0. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) J. Biol. Chem. 193,265-275.
  16. Konijn, T. M. (1970) Experientia 26,367-369.
  17. Wurster, B., Schubiger, K., Wick, U. & Gerisch, G. (1977) FEBS Lett. 76, 141-144.
  18. Gerisch, G., Hulser, D., Malchow, D. & Wick, U. (1975) Phil. Trans. R. Soc. London. Ser. B 272, 187-192.
  19. Gerisch, G., Maeda, Y., Malchow, D., Roos, W., Wick, U. & Wurster, B. (1977) in Development and Differentiation in the Cellular Slime Moulds, eds. Cappuccinelli, P. & Ashworth, J. M. (Elsevier, Amsterdam), pp. 105-124.
  20. Roos, W. & Gerisch, G. (1976) FEBS Lett. 68, 170-172.
  21. Mato, J. M. & Malchow, D. (1978) FEBS Lett. 90, 119-122.
  22. Hazelbauer, G. L. (1979) Nature (London) 279, 18-19.
  23. Mato, J. M. (1979) FEBS Lett. 102,241-243.
  24. Strittmatter, W. J., Hirata, F. & Axelrod, J. (1979) Biochem. Biophys. Res. Commun. 88,147-153.
  25. Pike, M. C., Kredich, N. M. & Snyderman, R. (1978) Proc. Natl. Acad. Scd. USA 75,3928-3932.