Towards Coleoptera-specific high-throughput screening systems for compounds with ecdysone activity: development of EcR reporter assays using weevil (Anthonomus grandis)-derived cell lines and in silico analysis of ligand binding to A. grandis EcR ligand-binding pocket (original) (raw)

A cell-based reporter assay for screening for EcR agonist/antagonist activity of natural ecdysteroids in Lepidoptera (Bm5) and Diptera (S2) cell cultures, followed by modeling of ecdysteroid-EcR interactions and normal mode analysis

Moisés Zotti

Pesticide Biochemistry and Physiology, 2013

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Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system - a basis for screening novel insecticidal candidates

Guy Smagghe

FEBS Journal, 2009

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Characterization and partial cloning of ecdysteroid receptor from a cotton boll weevil embryonic cell line

Tarlochan Dhadialla

Archives of Insect Biochemistry and Physiology, 1997

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High-throughput screening of ecdysone agonists using a reporter gene assay followed by 3-D QSAR analysis of the molting hormonal activity

Guy Smagghe

Bioorganic & Medicinal Chemistry, 2006

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Requirement of co-factors for the ligand-mediated activity of the insect ecdysteroid receptor in yeast

Salam Shaaban

Journal of Molecular Endocrinology, 2001

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Cloning, ligand-binding, and temporal expression of ecdysteroid receptors in the diamondback moth, Plutella xylostella

Xiwu Gao

BMC Molecular Biology, 2012

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Unprecedented conformational flexibility revealed in the ligand-binding domains of the Bovicola ovis ecdysone receptor (EcR) and ultraspiracle (USP) subunits

Tram Phan, George Lovrecz

Acta Crystallographica Section D-Biological Crystallography, 2014

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Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata

Yoshiaki Nakagawa

Febs Journal, 2005

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An Immunophilin is a Component of the Insect Ecdysone Receptor (EcR) Complex

qisheng song

Insect Biochemistry and Molecular Biology, 1997

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Ecdysone Receptor Present in Insects is a Novel Target for Insecticide

Neeru Redhu

International Journal of Current Microbiology and Applied Sciences, 2018

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Forest insect cell lines responsive to 20-hydroxyecdysone and two nonsteroidal ecdysone agonists, RH-5849 and RH-5992

Arthur Retnakaran

Journal of Insect Physiology, 1995

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Highly Flexible Ligand Binding Pocket of Ecdysone Receptor: A SINGLE AMINO ACID CHANGE LEADS TO DISCRIMINATION BETWEEN TWO GROUPS OF NONSTEROIDAL ECDYSONE AGONISTS

Mitch Polley

Journal of Biological Chemistry, 2004

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Complex steroid-peptide-receptor cascade controls insect ecdysis

Ingrid Zitnanová

General and comparative …, 2007

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Ecdysteroid ligand-receptor selectivity - exploring trends to design orthogonal gene switches

Anna Barlow

FEBS Journal, 2008

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The X-ray Structure of a Hemipteran Ecdysone Receptor Ligand-binding Domain

Dave Winkler

Journal of Biological Chemistry, 2005

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A cell-based high-throughput screening system for detecting ecdysteroid agonists and antagonists in plant extracts and libraries of synthetic compounds

Silvia Ciolfi

Faseb Journal, 2003

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Comparative effects of a non-steroidal ecdysone agonist RH-5992 and 20-hydroxyecdysone in a lepidopteran cell line (IAL-PID2)

Stéphane Debernard

Insect Biochemistry and Molecular Biology, 2005

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Functions of the ecdysone receptor isoform-A in the hemimetabolous insect Blattella germanica revealed by systemic RNAi in vivo

Xavier Belles

Developmental Biology, 2006

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Cloning and Characterization of Ecdysone Receptor and Ultraspiracle cDNAs from Spodoptera litura

Manolis Mavroidis

Annals of the New York Academy of Sciences, 2005

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Structural changes under low evolutionary constraint may decrease the affinity of dibenzoylhydrazine insecticides for the ecdysone receptor in non-lepidopteran insects

Pierre Rouge

Insect Molecular Biology, 2012

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Ecdysteroid signaling in ecdysteroid-resistant cell lines from the polyphagous noctuid pest Spodoptera exigua

Hadi Mosallanejad

Insect Biochemistry and Molecular Biology, 2008

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Comparative toxicity and ecdysone receptor affinity of non-steroidal ecdysone agonists and 20-hydroxyecdysone in Chironomus tentans

Guy Smagghe

Insect Biochemistry and Molecular Biology, 2002

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