Biogenic Synthesis and Characterization of Antioxidant and Antimicrobial Silver Nanoparticles Using Flower Extract of Couroupita guianensis Aubl (original) (raw)
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The present investigation demonstrates Cour-oupita guianensis flower buds extract mediated synthesis of stable silver nanoparticles (AgNPs). Instant formation of AgNPs was primarily confirmed by the appearance of yellowish brown colour and characteristic silver SPR band in the UV–visible spectrum. Elemental and crystalline natures of the AgNPs were identified from EDX and XRD pattern, respectively. Spherical morphology and the mono-disparity were revealed from TEM and AFM images. The particle size ranged from 5 to 30 nm and average size of 17 nm was consistent in XRD, TEM and AFM measurements. Possible reduction and stabilizing agents, viz., phenolics, flavonoids and proteins were identified from the characteristic FTIR peaks representing their functional groups. The strong antibacterial activity of synthesized AgNPs against Gram-positive and Gram-negative bacteria exhibited the potential for the formulation of synergistic bactericides by combining antibacterial properties of Couroupita flower buds extract and silver salts for biomedical applications.
Asian Journal of Pharmaceutical and Clinical Research, 2020
Objective: In this study, a rapid and simple approach was applied for the synthesis of silver nanoparticle (AgNP) from aqueous leaf extract of Couroupita guianensis. The plant extract acts as an antimicrobial agent and is also used to synthesis AgNP. Methods: Ultraviolet (UV)-visible spectrophotometer was used to characterize synthesized AgNP and to identify the compounds responsible for the reduction of silver ions, the functional groups present in plant extract were investigated by Fourier transform infrared. Agar well diffusion method was used for the antibacterial activity of synthesized AgNPs. Results: UV-visible spectrophotometer showed an absorbance peak in the range of 405 nm. The AgNPs showed antibacterial activities against Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, Bacillus sp, Klebsiella sp, and Pseudomonas sp. Conclusion: Nowadays, silver-based topical dressing has been widely used as a treatment for infections such as wounds, burns, and chronic ulcer.
1. ABSTRACT Green synthesis of nanoparticles in pharmaceuticals has gained much interest due cost-effective, environmental friendly and non-hazardous approach in the procedures. In this study, a method for green synthesis of silver noanoparticles from Couroupita Guianensis was established and evaluated for its antibacterial activity. The silver nanoparticles were prepared from different concentrations of silver nitrate to obtain maximum yield. The formation of the silver nanoparticles was confirmed by UV-Visible range spectroscopic analysis. The synthesized silver nanoparticles were characterized for its size and morphology by SEM and TEM analysis. The SEM and TEM revealed green synthesized nanoparticles were monodispersed, polycrystalline and polyhedral in the nature. The FTIR spectrum confirmed the interaction of the plant phytoconstituents with the silver nanoparticles which act as capping agent for the silver nanoparticle and enhance its shelf-life. The green synthesized silver nanoparticles possessed efficient antibacterial activity against Gram negative bacteria, E. coli and Pseudomonas aeruginosa. 2.
Industrial Crops and Products, 2014
A rapid method for synthesis of silver nanoparticles using the leaf extracts of Caesalpinia coriaria that has high level of tannin contents is reported. For leaf extracts preparation, two methods were adopted and compared with their extracellular synthesis of metallic silver nanoparticles. Stable silver nanoparticles produced by treating an aqueous solution of AgNO 3 (5 mM) with the leaf extracts as reducing agent of Ag + to Ag 0. The formation of the silver nanoparticles (AgNPs) in the reaction mixture was determined using the surface plasmon resonance band shown in UV-vis spectroscopy. To identify the active biomolecules responsible for the reduction of Ag + ion and the stabilization of AgNPs synthesized the functional group available in C. coriaria leaf extracts (CCLE) was studied by Fourier transmission infrared (FT-IR) spectroscopy. The X-ray diffraction (XRD) analysis showed the crystalline structure of metallic silver ions. The energy dispersive X-ray spectroscopy (EDX) analysis of AgNPs was confirmed the presence of pure elemental silver ion with no other impurity peaks. The morphology and topography of the AgNPs were determined using field emission scanning electron microscopy (FESEM). The size of the silver nanoparticles was differed by the kind of leaf extracts used for synthesis. The morphology of the AgNPs synthesized using boiled leaf extracts was found to be triangle shape with diameter range from 40 nm to 52 nm whereas in the case centrifuged leaf extracts, three shapes viz., triangle, hexagonal and spherical were noticed with an average size between 78 nm and 98 nm. Furthermore the synthesized AgNPs were found to show potential antimicrobial activity against multidrug resistant Gram-positive (Escherichia coli and Pseudomonas aeruginosa) and Gram-negative (Klebsiella pneumoniae, and Staphylococcus aureus) clinically isolated human pathogens. The present results clearly indicated that the CCLE is a good nontoxic source for extracellular synthesis of AgNPs in eco-friendly way as compared to other methods. Thus C. coriaria leaf extracts provide a potential source for development of commercially important industrial products including cosmetics and nanomedicines by tagging nanoparticels with tannins in the future.
Synthesis of Biogenic Silver Nanoparticles From Medicinal Plant And It’s Antibacterial Activity
IOSR Journal of Applied Chemistry, 2016
Biological synthesis of nanoparticles from medicinal plant is gaining importance due to its ecofriendliness. The synthesis of metal nanoparticles is a broad research area due to the potential applications for the development of different novel technologies. In our research work, we have described a cost effective and eco-friendly technique for the green synthesis of silver nanoparticles and evaluated their Antibacterial activity. Synthesis and characterization of silver nanoparticles were carried out by using Crateva religiosa plant bark extract as reducing agent as well as capping agent. The Synthesized nanoparticles were characterized with UV-Visible spectrometry(UV-Vis), Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and X-ray Diffraction Spectroscopy(XRD). The antibacterial activity of silver nanoparticles has been Observed.
Zenodo (CERN European Organization for Nuclear Research), 2020
Silver nanoparticles (AgNPs) are gaining attention because of its wide application in medical science as antimicrobial and antibacterial properties. A conventional method for synthesis of AgNPs consumes hazardous chemicals and during production of NPs harmful by-products may also form. Proposed review suggests "greener technology" for synthesis of silver nanoparticles with the help of easily available plants. Aqueous extract of leaf or roots of Impatiens balsamina, Lantana camara, Eriobotrya japonica, Berberis vulgaris, Azadirachta indica, Psidium guajava, Moringa oleifera and Catharanthus roseus and other plant extracts are used as reducing agents to convert Ag + into Ag 0. Light or faint color of plant extract after addition of AgNO 3 turns to brown or deep brown in color, indicates the formation of AgNPs. Fourier Transform Infra Red Spectroscopy (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Tunneling Electron Microscopy (TEM) and Dynamic Light Scattering Spectroscopy (DLSS) are techniques employed for characterization and morphological analysis of synthesized silver nanoparticles. Antibacterial, antifungal and antiparacitic applications of synthesized AgNPs have been also discussed. It is concluded that AgNPs synthesized by green way has great potency towards both Gram-negative and Gram-positive bacteria. Moreover, green synthesis of silver nanoparticles is a nonhazardous, eco-friendly, economic and safe technique and will envisaged better option for synthesis of AgNPs.
Extracellular in situ synthesis of metal nanoparticles using extracts of plants like Azadirachta indica (Neem), and Zingiber officinale (Ginger) has been successfully carried out earlier. In this study we have developed a novel method to synthesize silver nanoparticles by the process of bio-chemical reaction between silver salt solution with seed extract of Coriandrum sativum without using any surfactant or external energy. By this method physiologically stable, bio-compatible Ag nanoparticles (NPs) were formed. These functionalized AgNPs could be used for targeted drug delivery with enhanced therapeutic efficacy and minimal side effects. It is well known that on treating the metallic salt solution with some plant extracts, a rapid reduction occur leading to the formation of highly stable metal nanoparticles. This method resulted in rapid synthesis of nanoparticles, i.e. reaction time was 1-2 h as compared to 2-4 days required by microorganisms. These nanoparticles were analysed by various characterization techniques to reveal their morphology and chemical composition. TEM image of these NPs indicated the formation of spherical, non-uniform, poly dispersed nanoparticles of mean size of 13.09 nm. A detailed study of anti-microbial activity of nanoparticles and its synergetic effectiveness with common narrow spectrum antibiotic has been successfully carried out.
Journal of Chemistry , 2020
Developments in nanotechnology and natural product research toward the search for novel antibacterial agents have drawn the interest of many scientists to the synthesis of silver nanoparticles (AgNPs) from natural product (especially plants) due to its numerous benefits over other methods of synthesis such as been easy, economical, convenient, and environmental friendly. Aside from the aforementioned advantages, the synthesis of AgNPs from medicinal plant has been reported as the best approach of synthesizing AgNPs with great biological activities due to the numerous biomolecules found in plants. Recently, the number of researches toward the improvement of the yield, morphological properties, analytical techniques, and the development of optimal conditions and exact mechanism for synthesizing AgNPs from plants have been increasing tremendously. In this review, we present a comprehensive report on the recent development in the synthesis, optimization conditions, mechanism, and characterization techniques of AgNPs synthesized from plant extracts. Furthermore, a thorough discussion on the recent advances in the application of AgNPs synthesized from plant as therapeutic agent against bacterial infections was made.
Interest in the biosynthesis of nanoparticles has increased in the last era by researchers. Nanoparticles have several applications in different fields like optoelectronics, magnetic devices, drug delivery, and sensors. Nanoparticle synthesis by green methods is safe for the environment and should be explored and encouraged popularly since various plants' have the high extent to form these nanoparticles. Worldwide, UV spectroscopy, Xray diffraction, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) besides Fourier Transform Infrared Spectroscopy (FTIR) are used in many ways for characterize nanoparticles. The most advantageous use of AgNPs is their great attribution to be used as antimicrobial agents. Finally, concept of AgNPs synthesis is deserved to be the modern technical and medical concern. The current review shows a complete comprehensive and analytical survey of the biosynthesis of AgNPs with a particular focus on their activities as antimicrobials and the possible theories of their effect on the microbial cell and all influenced secondary metabolites.
Biosynthesis and Characterization of Silver Nanoparticles
Research Square (Research Square), 2022
In this study, a quick, simple, cost-e cient, and green procedure for silver nanoparticles (AgNPs) biosynthesis was executed at 25°C using 5 easily accessible plants from Cameroon including Carica papaya, Achillea millefolium, Perilla frutescens, Ocimum gratissimum, and Garcinia kola. These plants served as capping and reducing agents, while the AgNO 3 salt was the precursor. Initially, bioreduction of metallic Ag + to Ag 0 nanoparticles was established via a reduction in pH. Biosynthesis of AgNPs was primarily a rmed visually via a color change of the reaction mixtures with the ultraviolet-visible (UV-Vis) spectroscopy absorption peaks demonstrating that the synthesized particles were indeed AgNPs. X-ray diffractometry (XRD) showed the nanoparticles were crystalline in nature and had negative zeta potential (ζ-potential) values, which indicate they could be naturally stable. The phytochemical and Fourier transform infrared (FTIR) spectroscopic analyses revealed the possible phytochemicals in each aqueous plant extract responsible for reducing the Ag + metallic ions to nanoparticles followed by capping and stabilization of the nanoparticles. The high-resolution transmission electrons microscopy (HRTEM) micrographs revealed nanoparticles of varying shapes and sizes. Also, micrographs from the scanning electron microscopy (SEM) showed clouds of polydispersed nanoparticles, which were con rmed by energy dispersive X-ray (EDX) spectroscopy to be highly composed of Ag, with strong optical peaks around 3 kV. The results thus validate that these AgNPs can be e ciently formulated using easily available tropical plants for safe applications in various sectors such as medicine and agriculture.