Multiple Deletions in Chromosome 3p are Associated with the Development of Head and Neck Squamous Cell Carcinoma (original) (raw)

Detailed deletion mapping was done in the chromosomal 3p21.2-22 and 3p12-13 regions, showing high deletions in our previous study, using 13 highly polymorphic microsatellite markers in 25 primary head and neck squamous cell carcinoma (HNSCC) to narrow down the candidate tumor suppressor genes’ (TSGs) loci. Deletions in the different regions of chr.3p were seen to increase significantly with the progression of the clinical stages as detected by the fractional regional loss (FRL) index analysis. Five discrete areas with the following order of deletion frequency i.e. D3: 3p21.31 > D2: 3p21.32 > D1: 3p21.33 > D4: 3p21.2-21.1 > D5: 3p12.1, had been identified. Among these regions, the onset of deletions during progression of the tumor i.e. from stage I to stage IV, was suggested to occur in the following order i.e. D3 → D1& D2 → D4 & D5. The deletion in the D5 region was significantly associated with the progression of the clinical stages. Microsatellite size alterations (MAs...