Colonic mucosa adjacent to adenomas and hyperplastic polyps?a morphological and histochemical study (original) (raw)
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Mucinous adenocarcinoma of the colon - a histochemical study
PubMed, 2011
Colorectal carcinoma is a major cause of cancer associated with a high rate of morbidity and mortality in the western world. One of the pathologic features considered to be important for prognostic is mucin production. Many authors confirmed that colon carcinomas with high mucin content tend to re-occur locally and carry a poor prognosis. For histochemical evaluation of mucin content, we investigated 149 patients who underwent surgical resection of sporadic colon adenocarcinomas, all over a 5-year period. For histological classification we used the WHO recommendation (2000) and to be more accurate we sub-classified mucinous adenocarcinomas by morphometrical analysis in three categories: pure mucinous, with extracellular mucin more than 80% of the tumoral volume; mixed type, with 50-80% extracellular mucin; and mixed type with less than 50% extracellular mucin. For histochemical investigation, we used stains such as: mucicarmine, PAS ÷ Alcian Blue and High Iron Diamine ÷ Alcian Blue. Our study proved the predominance of mixed mucinous adenocarcinomas with less than 50% extracellular mucin, followed by the pure mucinous type. From the biochemical composition's point of view, the predominant cases were those with acidic mucins, especially in pure mucinous adenocarcinomas (>90%), while those with mixtures of acidic and neutral mucins were present in 62% of the cases. In addition, our study showed the prevalence of sialomucins over sulphomucins (68%), particularly in pure mucinous adenocarcinomas (77%). Clinical pure mucinous forms were detected mainly in advanced stages, but in terms of lymph node metastasis rate, they were secondary after mixed type with 50-80% extracellular mucin.
Mucin secretion and morphological changes of the mucosa in non-neoplastic diseases of the colon
Histopathology, 1983
Mucin secretion and morphological changes of the mucosa in non-neoplastic diseases of the colon Changes in mucin secretion and increase in height of the colonic mucosa adjacent to colorectal carcinoma (transitional mucosa) have been considered pre-malignant. In this study similar changes (both morphological and histochemical) have been found in some cases of ulcerative colitis and ischaemic colitis, as well as in juvenile, inflammatory and hyperplastic (metaplastic) polyps. 'Transitional' patterns of mucin secretion also occur in some other cases of ulcerative colitis, colostomies and Crohn's disease of the colon in which the mucosa has a normal height, suggesting the changes in much secretion are independent of mucosal morphology. In all these pathological conditions, hyperplastic (metaplastic) mucosa also coexisted. These findings seem to suggest that : ( I ) 'transitional' changes more likely represent a secondary regenerative phenomenon rather than a premalignant one; (2) the pattern of mucin secretion is not selective enough to serve as a premalignant marker; therefore is not a valid prognostic indicator in colonic biopsies ;
Mucin profiles in the mucosa adjacent to large bowel non-adenocarcinoma neoplasias
Histopathology, 1984
Morphological features and mucin secretion patterns were investigated in the colonic mucosa adjacent to or overlying mesenchymal or primary epithelial neoplasias, other than adenomas and adenocarcinomas. The material included 15 cases of non-adenocarcinoma tumours examined during 1978-1981. Increased sialomucins and morphological features similar to those described in the so-called 'transitional' mucosa adjacent to primary colorectal adenocarcinomas were observed in only two cases. In contrast our previous studies have demonstrated 'transitional' profiles in 98 % of adenocarcinomas.
Mucin histochemical profile in a variety of human colonic non-neoplastic and neoplastic lesions
IP innovative publication pvt. ltd, 2019
Introduction: Mucin deregulation occurs in gastrointestinal lesions progressing to cancer. Evidences advocate utility of mucin profile to predict malignant transformation in preneoplastic colonic lesions, however data are controversial. Objectives: This clinicopathological descriptive study identifies the histochemical profiles of acidic and neutral mucins in human colonic tissues including inflammatory, non-neoplastic and neoplastic lesions. Mucin alteration in different histological grades of adenocarcinoma is also evaluated. Materials and Methods: After collection of relevant data, tissue samples from a cohort of 88 patients recruited from King Abdul Aziz Specialized Hospital, Taif, Saudi Arabia were examined. Dual Alcian Blue (AB; pH 2.5)/ Diastase (D) Periodic Acid Schiff (PAS) technique was used to differentiate acidic and neutral mucins respectively. Statistical analysis including descriptive statistics, Pearson’s chi-square test and linear regression was performed using SPSS. Results: Acidic and neutral mucins were positive in 67.1 and 46.6% of lesions, respectively. All ulcerative colitis tissues were positive for acidic mucin and 75% for neutral mucin. All non-neoplastic polyps were positive for both mucins. Adenomatous polyps demonstrated reduced mucin but more neutral (59.3%) than acidic one (44.4%). About 86% of adenocarcinomas revealed acidic mucin and 22.2% showed neutral mucin with trends for acidic mucin to decrease with grade and for neutral mucin to increase with grade. Conclusion: Altered both acidic and neutral mucin profiles occur in colonic lesions of different pathologies. As colonic adenocarcinoma progresses from grade 1 to grade 3, acid mucin declines with a corresponding increase in neutral mucin. Mucin profile may be of value in evaluating the preneoplastic colorectal lesions.
Tumori Journal, 1984
We studied 393 endoscopic colonic biopsies from 72 patients, classified in different groups after assessment of their risk factors, using: histologic, histochemical and immunohistochemical techniques (CEA-PAP and PNA-Px). In high risk patients (Groups A and B) the most relevant modifications were dysplasia with hyposecretion and hyperplasia with sialomucin secretion (TR-type change); in Group A 31 adenomas and 19 hyperplastic polyps also were found. A premalignant nature of dysplasia and predysplastic significance of TR change were suggested: 1) by finding the same alterations in mucosa of Group E (colectomized patients for carcinoma at after 1 year); 2) by increasing expression of CEA and PNA-Px ligands; 3) by their absence in Group D (normal controls). Hyperplastic polyps were confirmed not to be premalignant lesions, but since they had peculiar characteristics (increased CEA and PNA-Px positivity, association with adenomas) it does not seem justified to regard them as lesions unr...
Acta Pathologica Microbiologica Scandinavica Series A :Pathology, 2009
in colorectal adenomas related to the presence of O-acylated sialic mucin and to morphometric measurements. Acta path. microbiol. immunol. xand. Sect. A, 95: 365-369, 1987. In a study of 41 surgically removed colorectal adenomas of unselected size, the dysplasia was graded as mild in 9, as moderate in 22, and severe in 10 cases. Twenty-four of the lesions were classified as tubular adenomas and 17 as tubulo-villous. The presence of 0-acylated sialic m u c h was for each of the adenomas assessed as normal (19 cases), intermediate (13 cases) or absent (9 cases). The Absence of 0-acylated mucin was in the present study found to be highly predictive for severe dysplasia (89%). The morphometric measurements of epithelial structures showed that nuclear shape factor and nuclear stratification index were most significantly related to the grades of dysplasia. In a multivariate analysis, the grading of O-cylated sialic m u c h most significantly contributed to the grade of dysplasia, and to a lesser degree, nuclear shape factor and nuclear stratification index. The presence of villous elements and the size of the adenomas had no independent influence on the grade of dysplasia in this statistical analysis.
Mucins in neoplastic spectrum of colorectal polyps: can they provide predictions
BMC Cancer, 2010
Background The significance of expression of different mucins in succession of malignant transformation of colorectal polyps is not determined yet. The aim of the present study was to determine the pattern of expression of MUC1, MUC2, MUC5AC and MUC6 in colorectal polyps and to evaluate the applicability of using mucin expression in predicting the extent of malignant transformation in colorectal polyps. Methods A total of 454 polyp specimens comprising 36 hyperplastic polyps, 15 serrated adenomas, 258 tubular adenomas, 114 tubulovillous adenomas, and 31 villous adenomas were included in this study, and were immunostained for MUC1, MUC2, MUC5AC and MUC6 by using mucin specific antibodies. Results MUC1 and MUC6 were absent in all hyperplastic polyps and their expression was higher in serrated and traditional adenomas. Only 5 cases including 2 serrated adenomas, 1 tubulovillous adenoma, and 2 villous adenomas stained negative for MUC2. The highest expression of MUC5AC was observed in serrated adenomas followed by tubular adenomas. Binary logistic regression analysis indicated that positive staining for MUC1, and MUC6, and negative staining for MUC2 would increase the risk of invasion to mucosa or the muscularis mucosae in colorectal polyps. Ordinal regression analysis demonstrated a positive association between the level of staining for MUC1 and risk of being of high configuration/grade in colorectal polyps. Conclusions MUC1, MUC2, MUC5AC, and MUC6 have the potential to be used as predictors of malignant transformation and invasion to mucosa or the muscularis mucosae in colorectal polyps. The most reliable predictions can be achieved by determining the level of expression of MUC1.
APMIS, 1991
of acid mucins in the descending colon of rats having simultaneously growing colonic tumors. APMIS 99: 993-996, 1991. The acid mucins contained in goblet cells of the descending colon of 34 male Sprague-Dawley rats were histochemically labeled by Alcian blue pH 2.5 and quantified in an image analyzer (Cortex Controller). Twenty-two of these 34 rats were treated with 1,2-dimethyIhydrazine (DMH) suspended in EDTA solution as a stabilizing agent and the remaining 12 rats with EDTA only. Of the 22 DMHtreated rats, 11 had concomitantly an adenocarcinoma elsewhere in the colon and the remaining 11 rats had no colonic tumors despite DMH treatment. The results indicated that Alcian blue-positive areas occupied 34.59'" of the mucosa of the descending colon in tumor-bearing rats, and 35.2% in non-tumor-bearing DMH-treated rats. For EDTA-treated rats the percentage of mucosa occupied by Alcian blue-positive cells was 48.1% The difference between DMH-treated rats (with or without tumors) and EDTA-treated rats was significant (p < 0.001). These results suggest that the decrease of Alcian blue areas is related to the protracted treatment with DMH. Whether the decrease in acid mucins is induced by the carcinogen per se or whether it represents a true biochemical premalignant change at the cytoplasmic level remains to be elucidated.