Amycolatopsis methanolica sp. nov., a facultatively methylotrophic actinomycete (original) (raw)
Related papers
Nocardia heshunensis sp. nov., an actinomycete isolated from soil
International Journal of Systematic and Evolutionary Microbiology
A novel Gram-stain-positive, aerobic, non-motile and acid-fast actinomycete strain, designated CFH S0067 T , was isolated from a soil sample collected from Heshun old town in Tengchong, Yunnan province, in southwest PR China. The taxonomic position of strain CFH S0067 T was studied in detail using a polyphasic approach. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain CFH S0067 T belongs to the genus Nocardia and is closely related to Nocardia concava JCM 12351 T (99.3 % similarity), forming a separated branch with this type strain. However, the strain shared 96.0 % gyrB gene sequence similarity with N. concava JCM 12351 T. Furthermore, DNA-DNA hybridization showed 56.5±0.6 % DNA relatedness between the novel strain and N. concava JCM 12351 T. The whole-cell hydrolysates contained mesodiaminopimelic acid (type IV) and arabinose, galactose, fructose and mannose. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid. Strain CFH S0067 T contained MK-8 (H 4!-cycl) as the predominant menaquinone. C 16 : 0 , summed feature 3 (C 16 : 1 !7c and/ or C 16 : 1 !6c), C 18 : 1 !9c and C 18 : 0 10-methyl (TBSA) were the major cellular fatty acids. Mycolic acids were also detected. The G+C content of the genomic DNA was determined to be 66.9 mol%. A combination of the low DNA-DNA hybridization values and phenotypic properties demonstrated that strain CFH S0067 T is clearly distinguishable from its most closely related strain, N. concava JCM 12351 T. On the basis of this polyphasic study, it is concluded that strain CFH S0067 T should be considered to represent a novel species of the genus Nocardia, for which the name Nocardia heshunensis sp. nov. is proposed. The type strain is CFH S0067 T (=DSM 46764 T =JCM 30085 T).
Amycolatopsis marina sp. nov., an actinomycete isolated from an ocean sediment
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2009
A Gram-positive, aerobic, non-motile actinobacterium, designated strain Ms392A T , was isolated from an ocean-sediment sample collected from the South China Sea. The isolate contained chemical markers that supported chemotaxonomic assignment to the genus Amycolatopsis. On the basis of an analysis of 16S rRNA gene sequence similarities, strain Ms392A T represents a novel subclade within the genus Amycolatopsis, with Amycolatopsis palatopharyngis 1BDZ T as its closest phylogenetic neighbour (99.4 % similarity). However, DNA-DNA hybridization demonstrated that strain Ms392A T was distinct from A. palatopharyngis AS 4.1729 T (48.6 % relatedness). The polyphasic analysis demonstrated that the ocean isolate can be clearly distinguished from recognized species of the genus Amycolatopsis. Therefore, strain Ms392A T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis marina sp. nov. is proposed. The type strain is Ms392A T (5CGMCC 4.3568 T 5NBRC 104263 T ).
A New Actinomycete Species, Nocardiopsis lucentensis sp. nov
International Journal of Systematic Bacteriology, 1993
A new species of the genus Nocardiopsis, for which we propose the name Nocardiopsis lucentensis sp. nov. (type strain, strain DSM 44048), was isolated from a salt marsh soil sample near Alicante, Spain. Whole-cell hydrolysates contain the meso isomer of diaminopimelic acid and no characteristic sugar; thus, the cell wall composition is type 111. Menaquinone MK-lO(H,) is the major menaquinone, and the phospholipid type is type PI11 (phosphatidylcholine present). Spore chains are rectiflexibilis, and in the early stages of sporulation zig-zag-shaped aerial hyphae are observed. This microorganism produces compatible solutes of the ectoine type and is characterized by a yellowish to yellowish brown substrate mycelium and a white aerial mycelium. This organism exhibits only 40 to 50% DNA relatedness to other Nocardiopsis spp.
Amycolatopsis rhabdoformis sp. nov., an actinomycete isolated from a tropical forest soil in Brazil
International journal of systematic and evolutionary microbiology, 2015
Strain SB026 T was isolated from Brazilian rainforest soil and its taxonomic position established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological features consistent with its classification in the genus Amycolatopsis and formed a branch in the Amycolatopsis 16S rRNA gene tree together with Amycolatopsis bullii NRRL B-24847 T , Amycolatopsis plumensis NRRL B-24324 T , Amycolatopsis tolypomycina DSM 44544 T and Amycolatopsis vancoresmycina NRRL B-24208 T . It was related most closely to A. bullii NRRL B-24847 T (99.0 % 16S rRNA gene sequence similarity), but was distinguished from this strain by a low level of DNA-DNA relatedness (~46 %) and discriminatory phenotypic properties.
2001
The taxonomic position of a group of moderately thermophilic actinomycetes isolated from vegetable matter was determined using a suite of genotypic and phenotypic properties. The organisms were found to share a range of chemical and morphological markers typical of members of the genus Amycolatopsis. A representative of the group, strain K24 T , formed a distinct phyletic line within the range of variation occupied by the genus Amycolatopsis in the 16S rDNA tree. The strains have many phenotypic properties in common and some of these distinguish the group from representatives of the validly described species of Amycolatopsis. It is clear from the combined datasets that the strains merit recognition as a new species of Amycolatopsis. The name proposed for the new species is Amycolatopsis sacchari ; the type strain is K24 T ( l DSM 44468 T l KCTC 9863 T ).
Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from Saharan soil
Antonie van Leeuwenhoek, 2014
An alkalitolerant actinomycete strain, designated B32 T , was isolated from a Saharan soil sample collected from Adrar province (South of Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce short chains of spores on the dichotomous branched aerial mycelium and formed a fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 0-5 % (w/v) and the optimum growth temperature and pH were found to be 25-35°C and 7.0-10.0°C, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain B32 T were identified as MK-10 (H 4 ) and MK-11 (H 4 ). The major fatty acids were found to be iso-C 16:0 and anteiso-C 15:0 . The diagnostic phospholipids detected were phosphatidylcholine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The chemotaxonomic properties of strain B32 T are consistent with those shared by members of the genus Nocardiopsis. 16S rRNA gene sequence analysis indicated that strain B32 T is most closely related to Nocardiopsis alba DSM 43377 T (98.7 %), Nocardiopsis lucentensis DSM 44048 T (98.6 %), Nocardiopsis aegyptia DSM 44442 T (98.6 %), Nocardiopsis sinuspersici HM6 T (98.6 %) and Nocardiopsis arvandica HM7 T (98.5 %). However, the DNA-DNA relatedness values between strain B32 T and the closely related type strains were 17.9, 14.6, 31.1, 27.1
Systematic and Applied Microbiology, 2000
The cephamycin producer strain 'Nocardia lactamdurans' contains four ribosomal RNA (rrn) operons. One of them (rrnD) was cloned from a DNA library in the bifunctional cosmid pJAR4. A 2229 bp region of rrnD has been sequenced. The 'N. lactamdurans' rrnD operon maintains the canonical order 5'-165-235-55-3'. Four of the consensus Giirtler-5tanisch sequences were found in the 165 rRNA gene and a fifth one in the sequenced 5' region of the 235 rRNA gene. The anti 5hine-Dalgarno sequence of 'N. lactamdurans' (located in the 3'-end of the 165 rRNA gene) was found to be 5'-CCUCCUUUCU-3' and is identical to that of Corynebacterium lactofermentum and Mycobacterium tuberculosis. A phylogenetic analysis of 'N. lactamdurans' by the neighbor-joining method using the entire 165 rRNA nucleotide sequence revealed that this actinomycete is closely related to Amycolatopsis orientalis subsp orientalis, Amycolatopsis coloradensis, Amycolatopsis alba, Amycolatopsis sulphurea and other Amycolatopsis sp. but only distantly related to species of the genus Nocardia. The cephamycin producer 'N. lactamdurans' NRRL 3802 should be, therefore, classified as Amycolatopsis lactamdurans. The deduced secondary structure of the 165 rRNA is very similar to that of A. colorandensis and A. alba but different from those of species of the Nocardia genus supporting the incorporation of 'N. lactamdurans' into the genus Amycolatopsis.