Assessment of Antimicrobial Activity from Saxifraga ligulata (original) (raw)

Phytomorphology: An International Journal of Plant Morphology

Benzene, petroleum-ether, chloroform and ethyl acetate extracts of Saxifraga ligulata were tested against three Gram positive bacteria viz., Bacillus subtilis, B. megaterium and Staphylococcus aureus, and five Gram negative bacteria viz., Escherichia coli, Salmonella typhimurium, Pseudomonas fluorescence, Ps. aeruginosa and Pantoea ananetis. The minimum inhibitory concentration (MIC) values of each fraction against all the bacteria were tested. Petroleum ether was found most effective, followed by ethyl acetate fraction. Both the fractions were found effective to kill all the bacteria tested, but at different doses. Benzene fraction was effective at very low concentration but was unable to produce any effect on Gram positive Staphyloccoccus aureus and Gram negative Escherichia coli. Morphology of the bacteria was severely affected when they were treated with sub lethal concentration of solvent fractions as revealed by the study of scanning electron microscopy. Also, the petroleum et...