Large-scale Top-down Proteomics of the Human Proteome: Membrane Proteins, Mitochondria, and Senescence (original) (raw)
AI-generated Abstract
Large-scale top-down proteomics is now a prominent technique for identifying proteins, evidenced by the identification of 1,220 proteins from the human cell line H1299 at a 1% false discovery rate. This study emphasizes the efficacy of using multiple separation strategies, particularly the focused isolation of mitochondria, leading to enhanced proteome coverage. The findings reveal significant insights into mitochondrial and membrane proteins, highlighting the ability of top-down proteomics to present intact proteoforms and their post-translational modifications, thereby advancing our understanding of cellular senescence and protein function.
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