Establishment of a dose-response curve for X-ray-induced micronuclei in human lymphocytes (original) (raw)

the recent past, additional assays have been worked out and validated including the now well-established cytokinesis-block micronucleus (CBMN) assay. Even though micronuclei (MN) can arise from exposure to a variety of clastogenic agents and are not necessarily radiation specific, ionizing radiation is considered as a clastogen and it is an efficient inducer of MN. The CBMN assay is established as a very reliable, thoroughly validated and is a standardized technique in the field of radiation biology to assess in vivo radiation exposure of occupational, medical, and accidentally exposed individuals and to determine individual in vitro radiosensitivity or cancer susceptibility. [2-4] To improve our cytogenetic laboratory capability as the National Cytogenetic Biodosimetry Laboratory in Indonesia, we have established our own dose-response standard curves of unstable chromosome aberrations for gamma radiation [5] and X-rays. [6] In vitro MN test with CBMN of human peripheral blood lymphocytes has been used extensively to study chromosomal damage induced by ionizing radiation or chemicals. [7] Because radiation-induced MN showed a radiation dose and quality dependence, MN can be used as a biological dosimeter for radiation protection purposes [8] and MN assay has been recommended by