Well Knowledge of the Physiology of Actinobacillus succinogenes to Improve Succinic Acid Production (original) (raw)
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Succinic Acid Production from Hexoses and Pentoses by Fermentation of Actinobacillus Succinogenes
Chemical Engineering Transactions, 2016
Succinic acid is a precursor for many chemicals in the food, pharmaceutical, cosmetic, and biodegradable plastics industries. Nowadays, the biotechnological route to produce succinic acid is focused on processes based on the fermentation of natural materials characterized by high fraction of carbohydrates. Actinobacillus succinogenes is proposed in the literature as a promising strain for the production of succinic acid. Indeed, A. succinogenes may utilize a wide spectrum of carbohydrates as carbon sources. Moreover, the CO2 required for the fermentation allows to include the biotechnological route among the carbon capture and conversion processes. A key issue of fermentation processes aimed at producing succinic acid is the feedstock cost. Pre-requisites of the feedstock are: availability at high mass flow rate over all the year, and non-competitive with (human and animal) food. Lignocellulosic biomass is a potential feedstock because it fulfils the listed pre-requisites. This cont...
Kinetic study of succinic acid production by Actinobacillus succinogenes ZT-130
Process Biochemistry, 2008
2.1. Culture strain A. succinogenes ZT-130 ATCC 55617 was used in this study. This microorganism was cultivated weekly in vials using trypticasein soy broth (Difco). 2.2. Cultivation The inoculum (80 ml, trypticasein soy broth sterile medium) was bubbled with CO 2 , incubated at 37 8C for 12 h and then transferred to a bioreactor (inoculum 10%, v/v). The formulation of the growth medium was 10 g/l yeast extract, 10 g/l of NaHCO 3 , 1 g/l of NaCl, 0.05 g/l of MgSO 4 , 6.8 g/l of K 2 HPO 4 , and 15.5 g/l of NaH 2 PO 4. All experiments were performed under the same conditions of pH, temperature, etc. The initial concentration of glucose in the growth medium was varied between 10 and 100 g/l. Fermentation was carried out in a glass bioreactor with a total volume of 1.2 l and initially containing 800 ml of growth medium. During the whole fermentation process, pH was controlled to 6.7 adding a solution of 2.5 M Na 2 CO 3. Temperature was maintained at 37 8C. The fermentation setup was agitated at 300 rpm and CO 2 gas was supplied at a flow rate of 0.4 vvm, for 12 h. After this time the CO 2 needed was supplied by a solution of 2.5 M Na 2 CO 3. 2.3. Analytical methods 2.3.1. Estimation of biomass concentration Biomass was quantified in terms of dry weight; the samples were centrifuged at 10,000 rpm for 15 min, afterwhich they were washed twice with distilled water and dried to a constant weight at 80 8C for 24 h.
Succinic Acid Production with Actinobacillus succinogenes ZT-130 in the Presence of Succinic Acid
Current Microbiology, 2010
Glucose fermentation with Actinobacillus succinogenes was carried out at different initial concentrations of succinic acid (SA 0 ) to determine its effect on growth and on the production of succinic acid itself. The specific rates of biomass production, succinic, formic and acetic acids decreased with SA 0 (0-40 g/l). The partially dissociated form of succinic acid had a higher effect on cell growth and production of succinic acid as compared to the non-dissociated forms of the acids, a fact that has not been reported until now. Cell growth fitted the Jerusalimski model, and the Aiba-Shoda model was suitable for quantification of the inhibition for the production of succinic acid. The growth inhibition constants K is and K ip and their summatory were consistent with the experimental values obtained, i.e., 22 g/l for the produced acids and 38 g/l for total acids that were the limits at which the biomass formation ceased.
Chiang Mai University Journal of Natural Sciences, 2016
This study used Actinobacillus succinogenes DSMZ 22257 to produce succinic acid using sorghum straw hydrolysate (SSH) as a low-cost carbon source. In anaerobic fermentation, the maximum succinic acid concentration of 52.180 g/l, corresponding to a yield of 0.870 g/g glucose was obtained from 60 g/l of glucose and faster cells growth was also observed. When using 40 g/l of SSH as a carbon source, succinic acid of 16.671 g/l, corresponding to yield of 0.777 g/g substrate was achieved after 24 h of cultivation. Statistical method: Plackett-Burman Design (PBD) was applied for a preliminary optimization of succinic acid fermentation medium by A. succinogenes DSMZ 22257. The highest succinic acid of 15.746 g/l was obtained with fermentation medium contained 50.0 g/l of yeast extract, 5.0 g/l of urea, 5.0 g/l of CaCl 2 , 0.25 g/l of MnCl 2 , 2.50 g/l of Na 2 CO 3 and 50 g/l of MgCO 3. The results from PBD, yeast extract and MgCO 3 were identified as the key medium components. The present study suggested that the renewable sorghum straw could be utilized as an alternative carbon source for succinic acid production. Further studies of the key medium components will be optimized using Response Surface Methodology to obtain optimum succinic acid production.
Continuous succinic acid fermentation by Actinobacillus succinogenes in a packed-bed biofilm reactor
Biotechnology for Biofuels, 2018
Background: Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by Actinobacillus succinogenes fermentation in a packedbed biofilm reactor (PBBR) was investigated. The effects of the operating conditions tested, dilution rate (D), and medium composition (mixture of glucose, xylose, and arabinose-that simulate the composition of a lignocellulosic hydrolysate)-on the PBBR performances were investigated. The maximum succinic acid productivity of 35.0 g L -1 h -1 and the maximum SA concentration were achieved at a D = 1.9 h -1 . The effect of HMF and furfural on succinic acid production was also investigated. HMF resulted to reduce succinic acid production by 22.6%, while furfural caused a reduction of 16% in SA production at the same dilution rate. Conclusion: Succinic acid production by A. succinogenes fermentation in a packed-bed reactor (PBBR) was successfully carried out for more than 5 months. The optimal results were obtained at the dilution rate 0.5 h -1 : 43.0 g L -1 of succinic acid were produced, glucose conversion was 88%; and the volumetric productivity was 22 g L -1 h -1 .
2012
i Synopsis Succinic Acid (SA) was continuously produced using glucose and a Mg2CO3(OH)2 slurry as feed. Glucose feed concentrations of 20 and 40 g ℓ -1 were employed with corresponding Mg2CO3(OH)2 slurry concentrations of 60 and 120 g ℓ -1 . The reactor pH was passively maintained between 6,4 and 6,8 by the buffer properties of the slurry in conjunction with the pH adjusted glucose feed. The suspended cell (SC) reactor was operated at 37°C with dilution rates varying between 0,04 h -1 and 0,6 h -1 . Groperl ® particles were used as inert supports in the biofilm reactor; dilution rates of 0,11 h -1 to 1 h -1 were investigated.
Biotechnology Reports, 2020
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BioResources
Soluble products from oil palm frond hydrolysate (OPFH) were incorporated as the fermentation substrate for succinic acid (SA) production. To construct a reliable kinetic model for the production of SA from OPFH, the inhibitory effects during fermentation involving glucose were evaluated based on the modified Gompertz model. The application of the modified Gompertz model was found to fit well with the experimental data for characterizing the SA fermentation profile and could predict the maximum potential of the metabolite formation in the form of concentrations, production rates, and initial lag times. The maximum potential of the SA production was determined to be 38.0 g/L, which was yielded from 61.3 g/L glucose in anaerobic batch cultivation after 30 h. The inhibitory effects on the cell growth and SA production became more apparent at higher substrate concentrations, which coincided with the substrate inhibition constant of 78.7 g/L. The model also helped in estimating the OPFH fermentation baseline, which consequently led to a SA concentration of 36.5 g/L and productivity of 1.95 g/Lh. The results suggested that OPFH as an inexpensive and renewable source of lignocellulosic syrup is advantageous for the economic production of SA.
Production of succinic acid by bacterial fermentation
Enzyme and Microbial Technology, 2006
Succinic acid produced by various microorganisms can be used as a precursor of many industrially important chemicals in food, chemical and pharmaceutical industries. The assessment of raw material cost and the estimation of the potential market size clearly indicate that the current petroleum-based succinic acid process will be replaced by the fermentative succinic acid production system in the foreseeable future. This paper reviews processes for fermentative succinic acid production, especially focusing on the use of several promising succinic acid producers including Actinobacillus succinogenes, Anaerobiospirillum succiniciproducens, Mannheimia succiniciproducens and recombinant Escherichia coli. Processes for the recovery of succinic acid from fermentation broth are also reviewed briefly. Finally, we suggest further works required to improve the strain performance suitable for successful commercialization of fermentative succinic acid production.