Comparing proliferative potential of rat mesenchymal stem cells derived from bone marrow and adipose tissue (original) (raw)

2015

Abstract

Background Mesenchymal stem cells (MSCs) received widespread attention because of their potential use in tissue engineering applications. Bone marrow (BM) had been extensively investigated as a source of adult stem cells. Adipose tissue (AT) is emerging as a source of stem cells that can be obtained by a less invasive method. Aim of the work The aim of the present study was to compare the MSCs derived from BM and AT in terms of their characterization, osteogenic differentiation, and proliferative capacity. Materials and methods In this study, 20 young albino rats were used. Their limb bones (femora and tibiae) and AT (from the inguinal groove) were extracted. MSCs were isolated from BM and AT, then cultured and subcultured to third passage. Characterization and osteogenic potentiality were determined for the cultured cells from both sources. Moreover, population doubling time was calculated, and then statistically compared to assess their proliferative potential. Results MSCs derive...

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