Isolation and Screening of Poly β-Hydroxybutyrate Producing Microbes (original) (raw)

Poly-3-hydroxybutyrate (PHB) is a natural biopolymer produce by wide range of microbes which is a storage granule used by microbes as an energy as well as carbon source. PHB exhibit stiffness, brittle to rubber like, biodegradable, environment friendly properties. In present work we were isolated 34 bacteria and 10 fungi at 37°C, 30°C for 3-5 and 5-7 days incubation respectively by using nutrient agar medium and potato dextrose agar medium from cardboard industry waste water and sugarcane farm soil. Among which 25 bacteria and 4 fungi shown PHB accumulation which was confirmed by Sudan Black B staining.

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