Bioactive Compounds and Antioxidant Capacity of Small Berries (original) (raw)
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Comparative study of the antioxidants content in some berries fruits
Scientific Bulletin Series F Biotechnologies, 2012
Berries fruits contain many different chemical compounds with different biological and pharmacological capacities and properties. So, these fruits are a source of bioactive molecules with antioxidant activity such as phenolic compounds, flavonoids, anthocyanins, ascorbic acid, that are important to human nutrition. Antioxidants neutralize free radicals, which cause oxidative damage to lipids, proteins, and nucleic acids and thus protect the organism. Research studies carried out on this topic indicated that natural antioxidants are involved in protection against many deseases: cancer, cardiovascular deseases, osteoporosis. The objective of this research was to determine and compare the antioxidants content offered by six berries fruits species: raspberry (Rubus idaeus), blackberry (Rubus fructicosus), strawberry (Fragaria ananassa), chokeberry (Aronia melanocarpa), blackcurrant (Ribes nigrum), sea-buckthorn (Hippophaea rhamnoides). The evaluation involved determination of total phenols, anthocyanins and flavonoids, using spectrophotometrical methods. Higher total phenol content levels were obtained in chokeberries and in blackcurrants.
Polyphenol content and antioxidant capacity of berries: A review, 2020
Nowadays in the world (especially in developed countries), increasing very important for human health, nutrition and protection from diseases has attracted consumer demand to foods and raw materials of high nutritional value. Berries, especially members of several families belonging to the best dietary sources of biochemical compounds. They have delicious taste and flavor, economic importance, and because of the antioxidant properties of some useful compounds, therefore, they are of great interest to researchers, nutritionists, and food technology experts. These compounds are responsible individually or in combination. For the various health benefits of berries in order to prevent the onset of chronic diseases and maintain health. In recent years, people's demand for healthy products has increased, in this way, many wild fruit species began to be cultivated in large areas. In particular, studies have focused on strawberries, currants, raspberries, blackberries, and blueberry due to their delicious taste and wide usage areas. These fruits contain high levels of phenolic compounds, anthocyanins, carotenoids, alkaloids, vitamins and have protective effects against health problems including degenerative diseases, cardiovascular diseases, diabetes, obesity and cancer and are recommended for a healthy diet. Such studies need to be developed extensively to detect and preserve important mulberry germplasm and to better understand the importance of such fruit species in the World. Berries, especially members of many families such as Rosaceae and Ericaceae are great dietary sources of biochemical compounds. these compounds (phenolic acids, flavonoids-flavonols, anthocyanins, tannins, and phenolic compounds such as ascorbic acid) are found in large amounts in berries and can function as potent antioxidants and therefore can help in the prevention and reduce of against many diseases including inflammation disorders, cardiovascular diseases, various cancers species. In summary, berries contain large amounts of phenolics and have also a high antioxidant activity and people use intensively in the last time for both healthy and their tastes.
Journal of Agricultural and Food Chemistry, 2005
Berries are known to contain phenolic substances (i.e., flavonoids and phenolic acids), which comprise two large and heterogeneous groups of biologically active nonnutrients. This investigation evaluated the content and profile of the phenolic compounds present in six different berries found in Northern Europe. The latter included bilberry (Vaccinium myrtillus), cowberry (Vaccinium vitis-idaea), cranberry (Vaccinium oxycoccus), strawberry (Fragaria ananassa), black currant (Ribes nigrum), and red currant (Ribes rubrum). The study was focused on two areas. The first involved the extraction and analysis of berries for total phenolic content and determination of their antioxidant activity. The total phenolic level of berries was correlated with their antioxidant activity. Second, the berry extracts were analyzed by capillary electrophoresis to determine the content and profile of selected bioactive compounds. The analytes of interest included trans-resveratrol, cinnamic acid, ferulic acid, p-coumaric acid, quercetin, and morin.
COMPARATIVE STUDY OF SOME BIOACTIVE COMPOUNDS AND THEIR ANTIOXIDANT ACTIVITY OF SOME BERRY TYPES
Bioactive compounds of berries, 2019
Berries are wealthy in bioactive compounds like phenolic compounds and flavonoids that are deemed antioxidants and are great important to health. This research was performed to examine, recognize and compare bioactive compounds in certain types of berries and their antioxidant activity. The data show that blue berry, black berry and Egyptian black mulberry contain the highest content of most bioactive compounds such as phenolic compounds, flavonoids and tannins, while long mulberry and red currant berry have the lowest content for most of these compounds. They therefore, contain the highest value of antioxidant activity. The chemical composition of the berries varies depending on cultivar, variety, location of growth, environmental conditions and harvest time, as well as post-harvest treatments therefore the composition differed from berry fruit to another. Thus, berry fruits are very useful in nutrition to protect the body from many diseases because of its containment of these compounds, which act as free radicals scavenger that harm the body and thus rid the body of many harmful toxins.
International Journal of Food Science & Technology, 2014
Two extractions with methanol and water were used to determine the antioxidant and binding properties of some berries as a supplement to food. Fluorometry, FTIR spectra and radical scavenging assays were used for characterisation of bioactive compounds (polyphenols, flavonoids, flavanols and tannins) and the levels of their antioxidant activities (AAs). The contents of bioactive compounds and AAs in water and methanol polyphenol extracts in gooseberries, blueberries and cranberries differed, but not always significantly. Water extracts of gooseberries showed the lowest amounts of polyphenols (mg GAE g À1 ), 6.24 AE 0.6, and flavonoids (mg CE g À1 ), 0.29 AE 0.01, and AAs (lMTE g À1 ) determined by DPPH, FRAP, ABTS and CUPRAC assays such as 6.05 AE 0.6, 8.07 AE 0.9, 18.70 AE 1.8 and 13.44 AE 1.2, respectively, in comparison with blueberries and cranberries. Polyphenol content highly correlated with antioxidant activity (R 2 from 0.94 to 0.81). The quenching properties of berries were studied by the interaction of water and methanol polyphenol extracts with HSA by 3D fluorescence. In conclusion, the bioactivity of gooseberries was lower than in blueberries and cranberries. Gooseberries can be used as a new source for food consumption and supplementation based on their antioxidant and binding properties. 3D fluorescence spectroscopy and FTIR spectroscopy can be applied as additional analytical tools for rapid estimation of the quality of different food products.
Phenolic Content and Their Antioxidant Activity in Various Berries Cultivated in Romania
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Food Science and Technology, 2015
Berry fruits are a rich source of phenolic compounds with health benefits. Phenolic compounds occur in berries mainly as a variety of conjugated forms, mostly with sugars. The aim of this work was to evaluate and compare the phenolic content and antioxidant potential in the most common fruits consumed in Romania: blueberry, blackberries, raspberry and cranberries. Folin-Ciocalteu method has been used in order to evaluate total phenolic content of analyzed berries. Antioxidant activity was determinate using ORAC assay which measures the decrease of AAPH-radical level by the scavenging action of the antioxidant substance. In addition, the vitamin C content and total tannins of the berries extracts were determined using spetophomotmetric methods. The phenolic contents and antioxidant potential of analyzed berries did not varied considerably. The highest amounts of TPC and the strongest antioxidant activities were found in blueberry and blackberries (678 GAE mg/100 g FW, 442 mg/100g FW respectively). Vitamin C content was found in higher concentration in raspberries 21.7 mg/100 g FW while the lower concentration was found in blackberry. All berries contain higher levels of bioactive compounds such as polyphenols or tannins which are responsible for their antioxidant potential and bring their nutritional value, being highly recommended for daily consumption.
Scientific Reports
The aim of this study was to determine the antioxidant activity, total phenolics, total flavonoid, proanthocyanidins, and anthocyanins content of eight berry species, namely serviceberry, gooseberry, blackberry, black chokeberry, bilberry, red currant, black currant, and cornelian cherry harvested in the regions of Sarajevo and Tuzla, Bosnia and Herzegovina. The antioxidant activity was determined by a battery of in vitro tests including DPPH radical assay, FRAP assay, ABTS assay, and phosphomolybdate test for total antioxidant capacity. Total phenolics, total flavonoids, and proanthocyanidins ranged from 0.834 to 6.921 mg TAE (tannic acid equivalents), 0.081–0.673 mg of quercetin, and 0.162–3.247 mg of catechin per gram of fresh fruit, respectively. The water extract of fruits had considerable levels of tested constituents and antioxidant activity, with the highest results obtained for black chokeberry. The multivariate clustering analysis showed that water extracts of analyzed spe...
IJRASET, 2021
There has been an increasing demand for the consumption of fruits, especially berries because of their nutraceutical value. Berries contain important bioactive compounds (BAC), which provide significant health benefits due to their antioxidant and antimicrobial effects. In this study, six different fresh and dried forms of berries were used to determine their phenolic, flavonoid content along with their antioxidant and antimicrobial potential. At first, the methanolic extraction of phenolic compounds was carried out and the extracts were further analysed. The total phenolic content was determined using Folin-Ciocalteu method and flavonoid content using aluminium chloride colorimetric method. Fresh blueberries extracts showed excellent results with 670.76µg/ml phenolic content and 27.27µg/ml flavonoid content. Antioxidant potential with value of 50.63µg/ml was observed highest in gooseberries extract, which was determined using FRAP assay. Antimicrobial activity of extracts showed maximum 37mm (fresh) and 32mm (dried) of zone of inhibition with gooseberries extract against Staphylococcus aureus. A comparative data of fresh and dried berries extracts was made, which showed fresh berries have higher activities in all parameters than dried berries extracts and thus, consumption of fresh berries are more fruitful. The use of berries which are an important source of BAC provides protection against harmful diseases. The phenolic compounds of berries are of great interest to nutritionist and food technologist due to the opportunity to use BACs as functional food ingredients.
Application of Analytical Methods for the Determination of Bioactive Compounds in Some Berries
Food Analytical Methods, 2013
ABSTRACT Fluorometry, ESI-MS, FTIR, and radical scavenging assays were used for characterization of bioactive compounds and the levels of their antioxidant activities. Polyphenols, flavonoids, anthocyanins, and ascorbic acid and the level of antioxidant activity of water extracts of "Murtilla-like" [Myrteola nummularia (Poiret) Berg.], and other widely consumed berries were determined and compared. The contents of bioactive compounds and the levels of antioxidant activities in water extracts differed significantly in the investigated samples (P < 0.05). "Murtilla-like" extracts contained polyphenols (mg GAE/g)-19.13 +/- 0.9, flavonoids, (mg CE/g)-3.12 +/- 0.1, anthocyanins (mg CGE/g)-120.23 +/- 5.4, and ascorbic acid (mg/g)-2.20 +/- 0.1; and antioxidant activities (mu molTE/g) by ABTS and CUPRAC assays were 200.55 +/- 8.7 and CUPRAC 116.76 +/- 5.7, respectively. Chemometrical processing was done on the basis of kinetic data of two variables (concentration and reaction time) by DPPH scavenging reaction. Polyphenol content highly correlated with antioxidant capacity (R-2 from 0.96 to 0.83). The quenching properties of berries were studied by the interaction of water polyphenol extracts with a small protein such as BSA by 3-D fluorescence and FTIR spectroscopy. These methods were used as additional tools for the characterization of polyphenols. Wild-grown non-investigated berries were compared with widely consumed ones, using their bioactive composition, antioxidant activities, and antiproliferative and fluorescence properties. In conclusion, the antioxidant properties of "Murtilla-like" can be used as a new source for consumption. The bioactivity of "Murtilla-like" is comparable with blueberries and raspberries. 3-D fluorescence and FTIR spectroscopy can be applied as additional analytical tools for rapid estimation of the quality of food products.