Hepatic Effects from Subacute Exposure to Insecticides in Adult Male Wistar Rats (original) (raw)

Abstract

Insecticides-Advances in Integrated Pest Management 280 2. Materials and methods 2.1 Materials Pluronic F-68 (20% purity) was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Malathion (98.5% purity), chlorpyriphos (99.6% purity), and deltamethrin (99.8% purity) were obtained from Sigma-Aldrich (SAF, Deisenhofen, Germany). CYP (91% purity; cis/trans, 49.9:50.1) was from Zeneca Co. (ICI of Mexico) and 4,4'-DDT (99% purity) was from Chem Service (West Chester, PA, USA). 2.2 Animals Seventy-six male Wistar rats were purchased from the University of Puebla farm, (Mexico), weighing 280-300 g and were acclimated for a minimum of 7 days before treatment. The animals were randomly selected, based on their body weight and housed in individual stainless-steel wire hanging cages during the 7 days of treatment. They were exposed to a 12-h light/dark cycle, at a room temperature of 19-22°C. The animals had free access to a rodent lab diet (Ralston Rations, Purina, St. Louis, MO) and water. Rats were divided into seven groups as shown in figure 1.

Loading...

Loading Preview

Sorry, preview is currently unavailable. You can download the paper by clicking the button above.

References (23)

  1. References
  2. Aldana, L., González de Mejía, E., Craigmill, A., Tsutsumi, V., Armendariz-Borunda, J., Panduro, A., & Rincón, AR. (1998). Cypermethrin increases apo A-1 and apo B mRNA but not hyperlipidemia in rats. Toxicol Lett, Vol.95, pp. 31-39, ISSN: 0378-4274
  3. Aldana, L., Tsutsumi, V., Craigmill, A., Silveira, MI., & González de Mejía, E. (2001). α- Tocopherol modulates liver toxicity of the pyrethroid cypermethrin. Toxicol Lett, Vol.125, pp. 107-116, ISSN: 0378-4274
  4. Aldana-Madrid, ML., Valdez-Hurtado, S., Vargas-Valdez, ND., Salazar-Lopez, NJ., Silveira- Gramont, MI., Loarca-Piña, FG., Rodriguez-Olibarria. G., Wong-Corral, FJ., Borboa- Flores, J., & Burgos-Hernández, A. (2008). Insecticide residues in stored grains in Sonora, Mexico: quantification and toxicity testing. Bull Environ Contam Toxicol, Vol.80, pp. 93-96, ISSN: 0007-4861
  5. CICOPLAFEST. Catálogo Oficial de Plaguicidas. Comisión Intersecretarial para el Control del Proceso y Uso de Plaguicidas, Fertilizantes y Sustancias Tóxicas. (2004). México, D.F. Pp. 33, 40, 46, 96.
  6. Eraslan, G., Kanbur, M., Silici, S., Altinordulu, S., & Karabacak, M. (2008). Effects of cypermethrin on some biochemical changes in rats: the protective role of propolis. Exp Anim, Vol.57, pp. 453-460, ISSN: 1341-1357
  7. Guillette, EA., Meza, MM., Aguilar, MG., Soto, AD., & García, IE. (1998). An anthropological approach to the evaluation of preschool children exposed to pesticides in Mexico. Environ Health Perspect, Vol.106, pp. 347-353, ISSN: 0091-6765
  8. Holland, PT., Hamilton, D., Ohlin, B., & Skidmore, MW. (1994). Effects of storage and processing on pesticide residue in plant products. Pure and Applied Chem, Vol.66, pp. 335-356. ISSN: 0033-4545
  9. Hussain, S., Khan, MZ., Khan, A., Javed, I., & Asi, MR. (2009). Toxico-pathological effects in rats induced by concurrent exposure to aflatoxin and cypermethrin. Toxicon, 53: 33- 41, ISSN: 0041-0101
  10. IFCC. International Foundation Chemical Clinical. (1986). BioMériux. IPCS. International Program of the Chemical Safety. The WHO recommended classification of pesticides by hazard and guidelines to classification: (2009). Pp 65-78. WHO Press, World Health Organization, 20 Avenue Appia, 1211 Geneva 27, Switzerland. (NLM classification: WA 240) ISBN 978 92 4 154796 3, ISSN: 1684-1042
  11. Lasram, MM., Annabi, AB., El Elj, N., Selmi, S., Kamoun, A., El-Fazaa, S., & Gharbi, N. (2009). Metabolic disorders of acute exposure to malathion in adult Wistar rats. J Hazard Mater, Vol.163, pp. 1052-1055, ISSN: 0304-3894
  12. Manna, S., Bhattacharyya, D., Mandal, TK., & Das, S. (2004).Repeated dose toxicity of alfa- cypermethrin in rats. J Vet Sci, Vol.5, pp. 41-45, ISSN: 1229-845X
  13. Meggs, WJ. & Brewer, KL. (2007). Weight gain associated with chronic exposure to chlorpyrifos in rats. J Med Toxicol, Vol.3, pp. 89-93, ISSN: 1523-5130
  14. Naraharisetti, SB., Aggarwal, M., Sarkar, SN., & Malik JK. (2008). Concurrent subacute exposure to arsenic through drinking water and malathion via diet in male rats: effects on hepatic drug-metabolizing enzymes. Arch Toxicol, Vol.82, pp. 543-551. ISSN: 0090-4341
  15. Narayan, S., Dani, HM., & Misra, UK. (1990). Changes in lipid profiles of liver microsomes of rats following intratracheal administration of DDT or endosulfan. J Environ Sci Health B, Vol.25, pp. 243-257. ISSN: 0360-1234
  16. Norma Oficial Mexicana (NOM-062-ZOO-1999). Especificaciones técnicas para la producción, cuidado y uso de los animales de laboratorio. Modificated: november 26, 2008. Available at www.senasica.gob.mx/default.asp?doc=743
  17. Pimentel Trevizan, LR., & Casadei de Baptista, G. (2000). Deltamethrin residues in wheat grains and their processed products evaluated by gas chromatography. Scientia Agricola, Vol. 57, pp. 199-203. ISSN 0188-4611
  18. RAPAM, A.C. Boletín de la Red de Acción sobre Plaguicidas y Alternativas en México. (1997). No. 17, Enero-Abril.
  19. Rezg, R., Mornagui, B., Kamoun, A., El-Fazaa, S., & Gharbi, N. (2007).Effect of subchronic exposure to malathion on metabolic parameters in the rat. C R Biol,Vol.330, pp. 143- 147. ISSN 1768-3238
  20. Rezg, R., Mornagui, B., El-Fazaa, S., & Gharbi, N. (2008). Biochemical evaluation of hepatic damage in subchronic exposure to malathion in rats: effect on superoxide dismutase and catalase activities using native PAGE. C R Biol, Vol.331, pp. 655-662. ISSN 1768-3238
  21. Saadi, L., Lebaili, N., & Benyoussi, M. (2008). Exploration of cytotoxic effect of malathion on some rat organs structure. Commun Agric Appl Biol Sci, Vol.73, pp. 875-81. ISSN: 1379-1176
  22. Shakoori, AR., Ali, RR., & Saleem, MA. (1998). Effects of six months' feeding of cypermethrin on the blood and liver of albino rats. J Biochem Toxicol, Vol.3, pp. 59-71. ISSN: 1095-6670
  23. Videira, RA., Antunes-Madeira, MC., Lopes, VI., & Madeira, VM. (2001). Changes induced by malathion, methyl parathion and parathion on membrane lipid physicochemical properties correlate with their toxicity. Biochim Biophys Acta, Vol.1511, pp. 360-368. ISSN 0005-2736