Hepatic Effects from Subacute Exposure to Insecticides in Adult Male Wistar Rats (original) (raw)

2012, Insecticides - Advances in Integrated Pest Management

Insecticides-Advances in Integrated Pest Management 280 2. Materials and methods 2.1 Materials Pluronic F-68 (20% purity) was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Malathion (98.5% purity), chlorpyriphos (99.6% purity), and deltamethrin (99.8% purity) were obtained from Sigma-Aldrich (SAF, Deisenhofen, Germany). CYP (91% purity; cis/trans, 49.9:50.1) was from Zeneca Co. (ICI of Mexico) and 4,4'-DDT (99% purity) was from Chem Service (West Chester, PA, USA). 2.2 Animals Seventy-six male Wistar rats were purchased from the University of Puebla farm, (Mexico), weighing 280-300 g and were acclimated for a minimum of 7 days before treatment. The animals were randomly selected, based on their body weight and housed in individual stainless-steel wire hanging cages during the 7 days of treatment. They were exposed to a 12-h light/dark cycle, at a room temperature of 19-22°C. The animals had free access to a rodent lab diet (Ralston Rations, Purina, St. Louis, MO) and water. Rats were divided into seven groups as shown in figure 1.